Glc4 (d -glucopyranosyl maltotriose, Glcα1-6Glcα1-4Glcα1-4Glc) was from TRC, Toronto, Canada). 13C6 hexose tetrasaccharide internal standard (6C13-labeled mixture of Glc4 and isomaltose-isomaltose, Glcα1-6Glcα1-4Glcα1-6Glc, IS) was a generous gift of Dr. S. Young (Duke Biochemical Genetics Laboratory, Durham, NC, USA). Sodium cyanoborohydride (NaBH3CN) and butyl-4-aminobenzoate (BAB) were from Sigma-Aldrich® (Saint Quentin-Fallavier, France). Acetonitrile (ACN) for HPLC, methanol and acetic acid were from Carlo-Erba (Val de Reuil, France). HPLC grade water was from Ecotainer Aqua B.Braun© (Melsungen, Germany). Bond Elut C18 50 mg, 1 mL solid phase extraction (SPE) columns were used with a Vac Elut 20 system, all from Varian (Agilent, Santa Clara, CA). Reverse phase LC was performed on a Uptisphere 3BioP2 C18 column (100 × 2.1 mm, 3 μm, ref. UP3BP2#10QS, Interchrom©, Interchim, Montluçon, France).
Vac elut 20 system
Manufactured by Agilent Technologies
The Vac Elut 20 system is a vacuum manifold for solid-phase extraction (SPE) and filtration. It provides a controlled vacuum environment for processing up to 20 samples simultaneously. The system is designed to facilitate sample preparation and extraction processes in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium cyanoborohydride nabh3cn, by Merck Group (1 mentions)
Acetic acid, by Carlo Erba (1 mentions)
3 hydroxy butanoic acid, by Merck Group (1 mentions)
Lichrolut en resin, by Merck Group (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
Triethylamine tea, by Merck Group (1 mentions)
Silica gel 60, by Merck Group (1 mentions)
2 protocols using vac elut 20 system
1
Analytical Glycan Quantification Protocol
2
Quantification of Hydroxy Acids via PFBBr Derivatization
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The standards of 2hydroxy2methylbutanoic acid (2OH2MB), 2hydroxy3 methylbutanoic acid (2OH3MB), 2hydroxy4methylpentanoic acid (2OH4MP), 3hydroxybutanoic acid (3OHMB), 2hydroxy2 methylpropanoic acid (2OH2MPr) and 2hydroxy3,3dimethylbutanoic acid (2OH33diMB) were purchased from SigmaAldrich and 3hydroxy3 methylbutanoic acid (3OH3MB) from Alfa Aesar, with purity higher than 95 % in all cases. 2,3,4,5,6Pentafluorobenzyl bromide (PFBBr) and tetrabutylamonium chloride (NBu Cl) (>97 %) were also purchased from Aldrich.
The solvents used were: Unisolv quality hexane (Hx), Lichrosolv quality ethanol, Suprasolv quality methanol (MeOH) and dichloromethane (DCM) and diethylic ether, all from Merck. Triethylamine (TEA) 95 % was from Aldrich. A milliQ purification system (Millipore, Bedford, MA, USA) provided pure water.
Sorbents used were: LiChrolut EN resins prepacked (200 mg, 3 mL reservoirs) and packed in house (200 mg in 1 mL reservoir) from Merck. SPE was performed with the help of a Vac Elut 20 system from Varian. Silicagel 60 from Merck was used for the purification process.
Standard solutions of the hydroxy acids were prepared directly in ethanol because of their insolubility in apolar solvents.
The solvents used were: Unisolv quality hexane (Hx), Lichrosolv quality ethanol, Suprasolv quality methanol (MeOH) and dichloromethane (DCM) and diethylic ether, all from Merck. Triethylamine (TEA) 95 % was from Aldrich. A milliQ purification system (Millipore, Bedford, MA, USA) provided pure water.
Sorbents used were: LiChrolut EN resins prepacked (200 mg, 3 mL reservoirs) and packed in house (200 mg in 1 mL reservoir) from Merck. SPE was performed with the help of a Vac Elut 20 system from Varian. Silicagel 60 from Merck was used for the purification process.
Standard solutions of the hydroxy acids were prepared directly in ethanol because of their insolubility in apolar solvents.
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