Glesatinib

Manufactured by Selleck Chemicals

Glesatinib is a type of laboratory equipment used for chemical analysis and research. It is a compact and durable instrument designed to measure and monitor various parameters in a laboratory setting. The core function of Glesatinib is to provide accurate and reliable data for researchers and scientists.

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Lab products found in correlation

2 protocols using glesatinib

Immunoblotting for Phospho-MET and HSP90

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Antibodies against phospho-MET (Tyr1234; sc-101736; RRID:AB_2143892) and HSP90 (sc-7947; RRID:AB_2121235) were purchased from Santa Cruz Biotechnology; total-MET (D1C2; #8198; RRID:AB_10858224) and anti-rabbit IgG-HRP (#7074; RRID: AB_2099233) from Cell Signaling Technology; Savolitinib, crizotinib, cabozantinib, and glesatinib were purchased from Selleckchem; merestinib from MedChem Express and Selleckchem. Chemical structures of the inhibitors are provided in Supplementary Fig. S1.

Bahcall M., Paweletz C.P., Kuang Y., Taus L.J., Sim T., Kim N.D., Dholakia K.H., Lau C.J., Gokhale P.C., Chopade P.R., Hong F., Wei Z., Köhler J., Kirschmeier P.T., Guo J., Guo S., Wang S, & Jänne P.A. (2021). Combination of type I and type II MET tyrosine kinase inhibitors as therapeutic approach to prevent resistance. Molecular cancer therapeutics, 21(2), 322-335.

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Cell Culture and Compound Screening Protocol

Cited 3 times

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NCI-H2077, RT112, DMS114, NCI-H520 were cultured in RPMI media, supplemented with 10% FBS, and penicillin/streptomycin/L-glutamine (PSG). AN3 CA was cultured in Dulbecco’s Modified Eagle Medium (DMEM), supplemented with 10% FBS and PSG. All cell lines were cultured at 37 °C in a humidified chamber in the presence of 5% CO2. Cell lines were obtained from ATCC or Sigma-Aldrich, primarily in 2014 and 2016, and were not further authenticated. Cells were not passaged for more than 6 months and were routinely monitored in our laboratory for cellular morphology and microbial presence by microscopic observation. Cell lines used for ORF screen, gene expression and xenograft studies were tested and confirmed negative for Mycoplasma (MycoAlert PLUS, Lonza).
BGJ398(26 (link)), trametinib(27 (link)), LDC1267(28 (link)), LOXO-101 (ARRY-470)(29 (link)), imatinib(30 (link)), BKM120(31 (link)), AZD8931(32 (link)) and MGCD265 (glesatinib)(33 (link)) were purchased from Selleck. FIIN-3(34 (link)) and Torin2(35 (link)) were a generous gift from Dr. Nathanael Gray at Dana-Farber Cancer Institute (Boston, MA).

Bockorny B., Rusan M., Chen W., Liao R.G., Li Y., Piccioni F., Wang J., Tan L., Thorner A.R., Li T., Zhang Y., Miao C., Ovesen T., Shapiro G.I., Kwiatkowski D.J., Gray N.S., Meyerson M., Hammerman P.S, & Bass A.J. (2018). RAS-MAPK reactivation facilitates acquired resistance in FGFR1-amplified lung cancer and underlies a rationale for upfront FGFR-MEK blockade. Molecular cancer therapeutics, 17(7), 1526-1539.

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