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Bio plex protm mouse cytokine assay

Manufactured by Bio-Rad
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Sourced in United States

The Bio-Plex Pro™ Mouse Cytokine Assay is a multiplex immunoassay that enables the simultaneous measurement of up to 23 different cytokines, chemokines, and growth factors in mouse biological samples. The assay utilizes magnetic beads coated with specific antibodies to capture and detect the analytes of interest.

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Lab products found in correlation

Bio plex 200tm system, by Bio-Rad (1 mentions)

Close spelling variants of this product

Bio-Plex (286 citations) Bio-Plex assay (73 citations) Bio-Plex Pro Mouse Cytokine (3 citations)

2 protocols using bio plex protm mouse cytokine assay

1

Quantification of BALF Cytokines

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Broncho-alveolar lavage fluid (BALF) was analyzed for secreted cytokines/chemokines using multiplex immunoassays. In brief, BALF samples were clarified by centrifugation (800 g for 5 min at 4°C) and the levels of cytokines quantitated using the Bio-Plex ProTM Mouse Cytokine Assay (Cat #: 12009159, Bio-Rad, Hercules, CA, USA). Recombinant cytokine samples were used as standards. Readings were performed on a Bioplex® 200TM system (Bio-Rad). Data were analyzed using Bio-Plex ManagerTM Software Version 6.0 Build 617 (Bio-Rad).
Zheng X., Wang K., Pan L., Hao W., Xue Y., Bacsi A., Vlahopoulos S.A., Radak Z., Hazra T.K., Brasier A.R., Tanner L., Ba X, & Boldogh I. (2022). Innate Immune Responses to RSV Infection Facilitated by OGG1, an Enzyme Repairing Oxidatively Modified DNA Base Lesions. Journal of Innate Immunity, 14(6), 593-614.
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2

Murine Serum Cytokine Profiling

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The presence in murine serum of IL-4, IL-6, IL-10, IL-13, IL-17, TGF-β, TNF-α, Keratinocyte-derived chemokine (KC), IFN-γ, IL-5, Monocyte chemoattractant protein-1 (MCP-1), IL-12p40, and IL-12p70 were quantified by Bio-Plex ProTM Mouse cytokine Assay following the manufacturer’s instructions (Biorad, Hercules, CA, USA). Serum was diluted 1:3 prior to analysis. Bio-Plex 200 system was employed to read and analyze the concentration of the cytokines.
Rizzo G., Pineda Chavez S.E., Vandenkoornhuyse E., Cárdenas Rincón C.L., Cento V., Garlatti V., Wozny M., Sammarco G., Di Claudio A., Meanti L., Elangovan S., Romano A., Roda G., Loy L., Dal Buono A., Gabbiadini R., Lovisa S., Rusconi R., Repici A., Armuzzi A, & Vetrano S. (2023). Pomegranate Extract Affects Gut Biofilm Forming Bacteria and Promotes Intestinal Mucosal Healing Regulating the Crosstalk between Epithelial Cells and Intestinal Fibroblasts. Nutrients, 15(7), 1771.
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