Analytical reference standards of 29 acyl-carnitines (Sigma-Aldrich, Co., St. Louis, MO, USA) were employed for quality control of compounds in samples. We defined acyl-carnitines and major acyl-carnitine fractions as free-, short-, medium-, or long-chain acyl-carnitines according to the length of carbon chains in the molecular structure (i.e., free carnitine: C0, short-chain: C2-C5, medium-chain: C6-C12, long-chain: C14-C18)29 (link),30 (link). Deuterium-labeled carnitine and acyl-carnitines (NSK-B Set, Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) were used as internal standards, including 2H9-carnitine (free carnitine, CN), 2H3-acetylcarnitine (C2), 2H3-propionylcarnitine (C3), 2H3-butyrylcarnitine (C4), 2H9-isovalerylcarnitine (C5), 2H3-octanoylcarnitine (C8), 2H9-myristoylcarnitine (C14), and 2H3-palmitoylcarnitine (C16). HPLC-grade ammonium acetate was purchased from Sigma-Aldrich (St. Louis, MO, USA); HPLC-grade formic acid and methanol were supplied by Thermo Fisher Scientific (Waltham, MA, USA); pure water was purchased from Wahaha Co., Ltd. (Hangzhou, China).
2h9 carnitine
Manufactured by Cambridge Isotopes
Sourced in United States
2H9-carnitine is a stable isotope-labeled compound used as an analytical standard for research purposes. It contains nine deuterium atoms, which can be used to track and quantify the compound in various analytical techniques.
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Lab products found in correlation
2h3 acetylcarnitine, by Cambridge Isotopes (2 mentions)
2h3 propionylcarnitine, by Cambridge Isotopes (2 mentions)
2h3 butyrylcarnitine, by Cambridge Isotopes (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Hplc grade formic acid, by Thermo Fisher Scientific (1 mentions)
Hplc grade ammonium acetate, by Merck Group (1 mentions)
2h9 isovalerylcarnitine, by Cambridge Isotopes (1 mentions)
2h3 octanoylcarnitine, by Cambridge Isotopes (1 mentions)
2h3 palmitoylcarnitine, by Cambridge Isotopes (1 mentions)
2h9 myristoylcarnitine c14, by Cambridge Isotopes (1 mentions)
Agilent 6460 triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions)
2 protocols using 2h9 carnitine
1
Analytical Reference Standards for Acyl-Carnitines
2
Acylcarnitine and Amino Acid Analysis
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Plasma acylcarnitines and amino acids were measured and analyzed by liquid chromatography and tandem mass spectrometry (LC-MS/MS) on an Agilent 6460 Triple Quadrupole Mass Spectrometer by the Mount Sinai Biochemical Genetic Testing Lab (now Sema4), as previously described (Le et al. 2014; Leandro et al. 2019; Ranea-Robles et al. 2020) . The L-carnitine internal standard ( 2 H 9 -carnitine) and the acylcarnitine internal standard mix containing 2 H 9 -carnitine, 2 H 3 -acetylcarnitine (C2), 2 H 3 -propionylcarnitine (C3), 2 H 3 -butyrylcarnitine (C4), 2 H 9 -isovalerylcarnitine (C5), 2 H 3 -octanoylcarnitine (C8), 2 H 9myristoylcarnitine (C14), and 2 H 3 -palmitoylcarnitine (C16), were from Cambridge Isotope Laboratories (Tewksbury, MA, USA).
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