The abdomen of mature individuals of both phases was swabbed (Heinz Herenz, Hamburg, Germany), followed by inserting each locust, head first, into a sterile 50 ml centrifuge tube (Corning, NY, United States) for 3 h, to collect its fecal pellets. This setup prevented the insects from moving, and ensured the collection of uncontaminated feces from each individual. Swab samples and the fecal pellets were marked as "t=0" preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted September 19, 2020. ; https://doi.org/10.1101/2020.09.17.301010 doi: bioRxiv preprint and kept in -80 C o until DNA extraction. Re-sampling at day 7 was conducted in the same manner; samples were marked "t=7" and stored at -80 C o until further use.
The copyright holder for this this version posted September 19, 2020. ; https://doi.org/10.1101/2020.09.17.301010 doi: bioRxiv preprint and kept in -80 C o until DNA extraction. Re-sampling at day 7 was conducted in the same manner; samples were marked "t=7" and stored at -80 C o until further use.