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Rabbit anti htert

Manufactured by Rockland Immunochemicals
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Rabbit anti-hTERT is a primary antibody that specifically binds to the human telomerase reverse transcriptase (hTERT) protein. hTERT is the catalytic subunit of the telomerase enzyme complex, which plays a crucial role in maintaining telomere length and cellular immortality.

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Lab products found in correlation

Lsm 510 meta, by Zeiss (2 mentions) Plan neofluar, by Zeiss (1 mentions) Mouse anti annexin 5, by Santa Cruz Biotechnology (1 mentions) Mouse anti hsp70, by Enzo Life Sciences (1 mentions)

2 protocols using rabbit anti htert

1

Immunofluorescence Imaging of hTERT in AFC

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AFCs were cultured on 11 mm glass coverslips until 60e80% confluence. IF was performed following primary antibody datasheet instructions (rabbit anti-hTERT, Rockland Immunochemicals Inc.). Confocal microscopy was performed on a ZEISS LSM 510 META confocal laser scanning microscope using the 63Â or the 100Â Plan-NEOFLUAR oil immersion objective. Single confocal sections are shown for each condition. Images were processed with the use of Image J [21] .
Radeghieri A., Savio G., Zendrini A., Di Noto G., Salvi A., Bergese P, & Piovani G. (2017). Cultured human amniocytes express hTERT, which is distributed between nucleus and cytoplasm and is secreted in extracellular vesicles. Biochemical and biophysical research communications, 483(1).
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2

Characterization of Extracellular Vesicles

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SDS sample buffer was added to isolated EVs or cellular extracts and samples were boiled 5 min at 95 C. Samples were electrophoresed in SDS-PAGE 10% and analyzed by Western-blot (WB) with rabbit anti-hTERT (Rockland Immunochemicals Inc.) [23] , mouse anti-Hsp 70 (Enzo Life Science), mouse anti-CD63 (Merck), mouse anti-Annexin-V (Santa Cruz) and/or mouse anti-Lamin A/C (Thermofisher) antibodies.
Radeghieri A., Savio G., Zendrini A., Di Noto G., Salvi A., Bergese P, & Piovani G. (2017). Cultured human amniocytes express hTERT, which is distributed between nucleus and cytoplasm and is secreted in extracellular vesicles. Biochemical and biophysical research communications, 483(1).
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