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2 protocols using anti mouse cd3

1

Murine T Cell Activation Assay

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FK506 (Cat. no. F4679-5MG), Concanavalin A (ConA) (Cat. no. C0412-5MG), 5 -IRTX (Cat. no. I9281-1MG) were purchased from Sigma Aldrich (St. Louis, MO, USA); antimouse CD25 (Cat. no. 553866), OptEIA kits for IL-2 (Cat. no.555148), , TNF (Cat. no. 558534) for sandwich ELISA were from BD Biosciences, SJ, CA, USA.; antimouse CD69 (Cat. no. 35-0691-U100), anti-mouse CD90.2 (Cat. no. 20-0903-U100) from Tonbo Biosciences, San Diego, CA, USA; anti-mouse TRPV1 (Cat. no. ACC-029) from Alomone Laboratories (Jerusalem, Israel); anti-mouse CD3 (Cat. no. 40-0032-U500) and antimouse CD28 (Cat. no. 40-0281-U500) [functional assay grade] were procured from Tonbo Biosciences, San Diego, CA, USA. Secondary anti-rabbit Alexa Fluor 488 and T cell isolation kit (Dynabeads™ Untouched™ Mouse T Cells Kit, Cat. no. 11413D) were procured from Invitrogen, Carlsbad, CA USA. RPMI-1640 cell culture medium and FBS were purchased from PAN Biotech, Aiden Bach, Germany; DMEM cell culture medium, 10X RBC lysis buffer, 10X PBS, L-glutamine, penicillin, streptomycin was from Himedia Laboratories, Mumbai, Maharashtra, India.
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2

Murine T Cell Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
FK506 (Cat. no. F4679-5MG), Concanavalin A (ConA) (Cat. no. C0412-5MG), 5 -IRTX (Cat. no. I9281-1MG) were purchased from Sigma Aldrich (St. Louis, MO, USA); antimouse CD25 (Cat. no. 553866), OptEIA kits for IL-2 (Cat. no.555148), , TNF (Cat. no. 558534) for sandwich ELISA were from BD Biosciences, SJ, CA, USA.; antimouse CD69 (Cat. no. 35-0691-U100), anti-mouse CD90.2 (Cat. no. 20-0903-U100) from Tonbo Biosciences, San Diego, CA, USA; anti-mouse TRPV1 (Cat. no. ACC-029) from Alomone Laboratories (Jerusalem, Israel); anti-mouse CD3 (Cat. no. 40-0032-U500) and antimouse CD28 (Cat. no. 40-0281-U500) [functional assay grade] were procured from Tonbo Biosciences, San Diego, CA, USA. Secondary anti-rabbit Alexa Fluor 488 and T cell isolation kit (Dynabeads™ Untouched™ Mouse T Cells Kit, Cat. no. 11413D) were procured from Invitrogen, Carlsbad, CA USA. RPMI-1640 cell culture medium and FBS were purchased from PAN Biotech, Aiden Bach, Germany; DMEM cell culture medium, 10X RBC lysis buffer, 10X PBS, L-glutamine, penicillin, streptomycin was from Himedia Laboratories, Mumbai, Maharashtra, India.
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