Chla 01

The Group 4 MB cell lines CHLA-01 and CHLA-01R, which derive from the primary tumor and a metastatic site, respectively, were purchased from the American Type Culture Collection (ATCC^® CRL3034^TM). Cells were cultured in suspension in DMEM:F12 Medium with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B27 Supplement to a final concentration of 2% (v/v), in a humidified atmosphere containing 5% CO₂ at 37 °C.
Luteolin and Dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich, St. Louis, MO, USA (Cat# L9283; Cat# D8418). Vincristine was obtained from Cayman Chemical Company, Ann Harbor, MI, USA (Cat# 11764).

Human MB cell lines CHLA01 and DAOY were purchased from the American Type Culture Collection, and D425 was purchased from Sigma-Aldrich (St. Louis, MO). STR profiling and Mycoplasma testing were performed on all cell lines. DAOY cells were cultured in Dulbecco’s Modified Eagle medium (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (FBS, Gibco), 1% sodium pyruvate, non-essential amino acids, and penicillin/streptomycin. D425 cells were cultured in DMEM/F12 with 10% FBS and 1% penicillin/streptomycin. CHLA01 cells were cultured in DMEM/F12 with B-27 supplement (Invitrogen, Waltham, MA), 20 ng/ml bFGF (R&D Systems, Minneapolis, MI), and 20 ng/ml EGF (R&D Systems). Cells were grown in a humidified incubator at 37 °C in 5% CO₂ and culture medium was replaced every 3 to 4 days. The cells were gently trypsinized (0.05%, Gibco) for subculture.