Niko digital dx1200me camera

Manufactured by Universal Imaging

The Niko Digital Dx1200me is a 12-megapixel digital camera designed for laboratory and scientific applications. It features a high-resolution sensor, advanced image processing capabilities, and connectivity options for integration with various imaging systems.

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Lab products found in correlation

Nis element, by Nikon (2 mentions) Ab133357, by Abcam (1 mentions) Tyr705, by Cell Signaling Technology (1 mentions) Ab15580, by Abcam (1 mentions) Nbp2 12506, by Novus Biologicals (1 mentions) Af1830, by R&D Systems (1 mentions) Ab3849, by Merck Group (1 mentions) Nis elements ar 2, by Nikon (1 mentions) Coolsnap hq camera, by Nikon (1 mentions) Endomucin, by Santa Cruz Biotechnology (1 mentions) Phospho histone h3, by Merck Group (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions)

2 protocols using niko digital dx1200me camera

Comprehensive Immunohistochemistry Protocol

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Formalin‐fixed, paraffin‐embedded tissues were cut in 5‐μm sections. Sections were evaluated by immunohistochemical analysis following our previously reported protocol (Sorrelle et al, 2019) using antibodies specific for anti‐P‐STAT3 (Tyr705, #9145, Cell Signaling, dilution 1:500), anti‐ARG1 (#93668, Cell Signaling, dilution 1:500), anti‐CD8α (#98941, Cell Signaling, dilution 1:1,000), anti‐ZAP70 (#2705, Cell Signaling, dilution 1:500), anti‐P‐SMAD2 (Ser465/467, AB3849, Millipore, dilution 1:500), anti‐IL‐6Rα (AF1830, R&D Systems, dilution 1:250), anti‐iNOS (PA1‐21054, Thermo Fisher Scientific, dilution 1:200), anti‐CD3 (PA1‐29547, Thermo Fisher Scientific, dilution 1:1,000), anti‐F4/80 (NBP2‐12506, Novus Biologicals, 1:200), anti‐FOXP3 (MAB8214, Novus Biologicals, dilution 1:500), anti‐CD11b (ab133357, Abcam, dilution 1:1,000), and anti‐Ki67 (ab15580, Abcam, dilution 1:1,000). Images were obtained with a Nikon Eclipse E600 microscope using a Niko Digital Dx1200me camera and ACT1 software (Universal Imaging Corporation). Pictures were analyzed using NIS Elements (Nikon).

Huang H., Zhang Y., Gallegos V., Sorrelle N., Zaid M.M., Toombs J., Du W., Wright S., Hagopian M., Wang Z., Hosein A.N., Sathe A.A., Xing C., Koay E.J., Driscoll K.E, & Brekken R.A. (2019). Targeting TGFβR2‐mutant tumors exposes vulnerabilities to stromal TGFβ blockade in pancreatic cancer. EMBO Molecular Medicine, 11(11), e10515.

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Immunohistochemical Analysis of Formalin-Fixed Tissues

Cited 1 time

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Formalin-fixed tissues were embedded in paraffin and cut in 5 μm sections. Sections were evaluated by H&E and immunohistochemical analysis using antibodies specific for vimentin (Cell Signaling, 5741), endomucin (Santa Cruz, 65495), E-cadherin (Cell Signaling, 3195), phospho-histone H3 (Millipore, 06-570), cleaved caspase-3 (Cell Signaling, 9664), equilibrative nucleoside transporter 1 (ENT1, Abcam, AB135756), F4/80 (Novus, NBP2-12506), Arginase 1 (Arg1, Santa Cruz, sc-18351). Negative controls included omission of primary antibody. Immunofluorescence evaluation was conducted as described (19 (link)). Fluorescent images were captured with a Photometrics CoolSNAP HQ camera using NIS Elements AR 2.3 Software (Nikon). Color images were obtained with a Nikon Eclipse E600 microscope using a Niko Digital Dx1200me camera and ACT1 software (Universal Imaging Corporation). Pictures were analyzed using NIS Elements (Nikon).

Ludwig K.F., Du W., Sorrelle N.B., Wnuk-Lipinska K., Topalovski M., Toombs J.E., Cruz V.H., Yabuuchi S., Rajeshkumar N.V., Maitra A., Lorens J.B, & Brekken R.A. (2017). Small molecule inhibition of Axl targets tumor immune suppression and enhances chemotherapy in pancreatic cancer. Cancer research, 78(1), 246-255.

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