Acetone (HPLC grade), acetic acid, chlorhydric acid (37%), 1-butanol, diethyl ether, ethanol (HPLC grade), petroleum ether, and 2,4-D herbicide standard were purchased from Sigma Aldrich (St Louis, MO, EE.UU.); acetonitrile, methanol (LC-MS grade) and ammonium hydroxide from Panreac AppliChem (Barcelona, Spain). Deionised water (18 MΩ·cm) was obtained with a purification system Millipore Milli-Q (Millipore, Bedford, MA, EE.UU.). Commercial 2,4-D herbicide was Esteron 60 (60% w/v, Dow AgroSciences).
Milli q millipore
Manufactured by Merck Group
Sourced in United States, Germany, Hungary
The Milli-Q Millipore is a water purification system that produces high-quality ultrapure water. It uses a multi-stage filtration process to remove impurities, ions, and organic matter from the input water, resulting in water that meets the highest purity standards.
Automatically generated - may contain errors
Lab products found in correlation
Sca20, by Dataphysics (4 mentions)
Contact angle system oca15 plus, by Dataphysics (4 mentions)
Formic acid, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Diethyl ether, by Merck Group (1 mentions)
Ammonium hydroxide, by ITW Reagents (1 mentions)
Acetonitrile, by ITW Reagents (1 mentions)
Methanol lc ms grade, by ITW Reagents (1 mentions)
Esteron 60, by Dow (1 mentions)
6 dodecanoyl 2 dimethylaminonaphthalene laurdan, by Thermo Fisher Scientific (1 mentions)
Capsaicin, by Merck Group (1 mentions)
Chloroform, by Kanto Chemical (1 mentions)
Methanol, by Kanto Chemical (1 mentions)
Zn no3 2 6h2o, by Tianjin Damao (1 mentions)
Thioacetamide, by Tianjin Kemiou Chemical Reagent (1 mentions)
Caffeic acid, by Extrasynthese (1 mentions)
Caftaric acid, by Extrasynthese (1 mentions)
Tyrosol, by Extrasynthese (1 mentions)
Ferulic acid, by Extrasynthese (1 mentions)
Gentisic acid, by Extrasynthese (1 mentions)
28 protocols using milli q millipore
1
HPLC-based Herbicide Extraction Protocol
2
Characterization of Lipid Membrane Probes
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DOPC, DPPC and Chol were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Rhodamine B 1,2-dihexadeconyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Rho-DPHE) (λex = 560 nm, λem = 580 nm) and 6-dodecanoyl-2-dimethylamino naphthalene (laurdan, λex = 430–455 nm, λem = 490–540 nm) were from Invitrogen (Carlsbad, CA, USA) and Funakoshi Co., Ltd. (Tokyo, Japan), respectively. Capsaicin was obtained from Sigma Aldrich Corporation (Tokyo, Japan). Chloroform and methanol were purchased from Kanto-Chemical Co., Inc. (Tokyo, Japan) and Nacalai Tesque, Inc. (Kyoto, Japan), respectively. All chemicals were of analytical grade and used as received. Deionized water was obtained using a Millipore Milli-Q (Millipore S.A.S., Molsheim, France), purification system, conductivity 18.2 MΩ cm−1.
3
Synthesis of S-Co-N/C ORR Catalyst
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The novel ORR catalyst of S-Co-N/C was prepared with the regents of Zn(NO3)2·6H2O (≥99 wt.%, Damao Chemical Reagent Factory), Co(NO3)2·6H2O (≥99 wt.%, Guangdong Guanghua Chemical Factory Co. Ltd.), 2-methylimidazole (Aladdin Industrial Corporation), methanol (≥99.5 wt.%, Tianjin Fuyu Fine Chemical Co. Ltd.), and thioacetamide (≥99 wt.%, Tianjin Kemiou Chemical Reagent Co. Ltd.). Potassium hydroxide (≥85 wt.%, Sichuan Xilong Chemical Co. Ltd.) was used as electrolyte during the ORR activity measurement. The reagents mentioned above were all analytical grade without any further purification. Ultrapure water (18.25 MΩ·cm−1 Millipore-Milli-Q) was used to prepare all the aqueous solutions in this work.
4
Quantitative Analysis of Phenolic Compounds
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The water used was purified in ultrapure Millipore Milli-Q (18.2 MΩ cm, Millipore, Merck KGaA, Darmstadt, Germany), methanol, ethanol, acetonitrile, formic acid, tartaric acid and trifluoroacetic acid were purchased from Interchim (Montluçon, France) at the highest grade of purity. A caustic soda solution at 50% (w/w) was supplied by Sigma-Aldrich (St. Louis, MO). Pure standards of gallic acid, protocatechuic acid, hydroxybenzoic acid, hydroxytyrosol, tyrosol, catechin, (2)-epicatechin, dimer B1 and B2, caftaric acid, gentisic acid, caffeic acid, coumaric acid, chlorogenic acid and ferulic acid were obtained from Extrasynthèse (Genay, France).
5
Peptide Deposition on Titanium Disks
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The peptides were dissolved in ultrapure water (Millipore Milli-Q, Merck Millipore Corporation, USA) at 100 μM and 100 μL of the solution were deposited on the Ti disks and incubated overnight at room temperature. Afterwards, all the samples were carefully cleaned with ultrapure water and dried with nitrogen.
6
Formulation and Characterization of MEL Delivery
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MEL (4-hydroxy-2-methyl-n-(5-methyl-2-thiazolyl)-2H-benzothiazine-3-carboxamide-1,1-dioxide) as a model drug was obtained from EGIS Ltd. (Budapest, Hungary). HSA (lyophilized powder, purity >97%), Tween 80 (Tween), phosphate buffered saline (PBS) powder, disodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (Na2HPO4), l -α-phosphatidylcholine (PC), and cholesterol (CHO) were purchased from Sigma Aldrich Co. Ltd. (Budapest, Hungary). The analytical grade solvents acetonitrile (MeCN), dimethyl sulfoxide (DMSO), dodecane, formic acid, and trifluoroacetic acid (TFA) were purchased from Merck KGaA (Darmstadt, Germany). Piroxicam, the internal standard for the HPLC method, was purchased from the Alfa Aeasar Co. (Alfa Aeasar GmbH & Co. KG, Karlsruhe, Germany). In all experiments, distilled water was purified using the Millipore Milli-Q® (Merck Ltd., Budapest, Hungary) 140 Gradient Water Purification System.
7
Monodisperse Polystyrene Microspheres Synthesis
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Styrene was purchased from Lingfeng Chemical Reagent Co., Ltd. (Shanghai, China). Sodium dodecyl sulfate (SDS) was supplied by Amresco (Shanghai, China). K2S2O8 (KPS, 99%), hexadecyltrimethylammonium bromide (99%), divinylbenzene (m- and p-mixture, 55% DVB in ethylene ethylbenzene (EVB) and diethyl benzene (DEB), stabilized with tert-butylcatechol (TBC)) were purchased from J & K Chemical (Shanghai, China). Sodium p-styrenesulfonate (95%) was purchased from Jiuding Chemical Reagent Co., Ltd. (Shanghai, China). The n-hexadecane (98%) was purchased from Shanghai Aladdin Industrial Co., Ltd. (Shanghai, China). Ethanol (99.7%), acetone (99.5%) and sodium chloride (99.5%) were purchased from Shanghai Titan Scientific Co., Ltd. (Shanghai, China). The n-decane (95%) was purchased from Shanghai Chaocong Chemical Co., Ltd. (Shanghai, China).
Styrene was stored in a refrigerator at 4 °C after removing the polymerization inhibitor via reduced pressure distillation. The homemade and high-efficiency photo initiator 2-[p-(2-hydroxy-2-methylpropiophenone)]-ethyleneglycolmethacrylate (HMEM) was synthesized as described in our previous work [30 (link)]. The water used for all experiments was purified by reverse osmosis (Millipore Milli-RO, Merck KGaA, Darmstadt, Germany) and subsequent ion exchange (Millipore Milli-Q, Merck KGaA, Darmstadt, Germany). All others were used as received.
Styrene was stored in a refrigerator at 4 °C after removing the polymerization inhibitor via reduced pressure distillation. The homemade and high-efficiency photo initiator 2-[p-(2-hydroxy-2-methylpropiophenone)]-ethyleneglycolmethacrylate (HMEM) was synthesized as described in our previous work [30 (link)]. The water used for all experiments was purified by reverse osmosis (Millipore Milli-RO, Merck KGaA, Darmstadt, Germany) and subsequent ion exchange (Millipore Milli-Q, Merck KGaA, Darmstadt, Germany). All others were used as received.
8
Bioactive Silane Surface Modification
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Sodium hydroxide (NaOH), triethoxysilylpropyl succinic anhydride silane (TESPSA), toluene, N,N-diisopropylethylamine (DIEA), isopropanol, ethanol, acetone and paraformaldehyde were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), phosphate buffered saline (PBS), fetal bovine serum (FBS), trypsin, L-glutamine, penicillin/streptomycin, palloidin-rhodamin, 4′,6-diamidino-2-phenylindole (DAPI) and LIVE/DEAD BacLight bacterial viability kit were purchased from Invitrogen (Carlsbad, CA, USA).
Cytotoxicity Detection Kit LDH was purchased from Roche Applied Science (Penzberg, Germany). Mammalian extraction reagent (M-PER®) was purchased from Pierce (Waltham, MA, USA). Streptococcus sanguinis was purchased from Colección Española de Cultivos Tipo (CECT, CECT 480, Valencia, Spain). Lactobacillus salivarius was purchased from the University of Göteborg Culture Collection (CCUG, CCUG 17826, Göteborg, Sweden). Todd-Hewitt (TH) broth and Man-Rogosa-Sharpe (MRS) broth were purchased from Scharlau (Scharlab SL, Sentmenat, Spain). Brain Heart Infusion Broth (BHI) was purchased from Difco (Detroit, MI, USA). The solutions were made with ultrapure water (Millipore Milli-Q, Merck Corporation, Kenilworth, NJ, USA).
Cytotoxicity Detection Kit LDH was purchased from Roche Applied Science (Penzberg, Germany). Mammalian extraction reagent (M-PER®) was purchased from Pierce (Waltham, MA, USA). Streptococcus sanguinis was purchased from Colección Española de Cultivos Tipo (CECT, CECT 480, Valencia, Spain). Lactobacillus salivarius was purchased from the University of Göteborg Culture Collection (CCUG, CCUG 17826, Göteborg, Sweden). Todd-Hewitt (TH) broth and Man-Rogosa-Sharpe (MRS) broth were purchased from Scharlau (Scharlab SL, Sentmenat, Spain). Brain Heart Infusion Broth (BHI) was purchased from Difco (Detroit, MI, USA). The solutions were made with ultrapure water (Millipore Milli-Q, Merck Corporation, Kenilworth, NJ, USA).
9
Solvent-based Carrier Systems for Megestrol
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The carrier forming materials D-Xylitol (XYL), β-D-mannitol (MAN), and polyethylene glycol 6000 (PEG 6000) were purchased from Sigma-Aldrich (Budapest, Hungary). The solubilizing agents Cremophor® RH 40 (CR 40) and Soluplus® (SP) were obtained from BASF GmbH (Ludwigshafen, Germany). Megestrol-acetate (Farmabios S. p. A., Pavia, Italy) was chosen as the model material for this experimental work. Analytical grade solvent methanol was also purchased from Sigma-Aldrich. Powders for the biorelevant gastric fluids were purchased from Biorelevant.com Ltd. (London, UK). Distilled water was purified for the experiments using the Millipore Milli-Q® (Merck Ltd., Budapest, Hungary) 140 Gradient Water Purification System.
10
Measuring Surface Wettability and Energy
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Static water contact angles were measured with distilled water (Millipore Milli-Q, Merck Millipore Corporation, USA) using the sessile drop method (Contact Angle System OCA15 plus; Dataphysics, Germany). Measurements were acquired in triplicate for three samples in each series at room temperature, with a volume of 3 µl and a dose rate of 1 µl/min.
Surface energy was also calculated with the Owens, Wendt, Rabel and Kaelble (OWRK) equation applied to both water and diiodomethane measurements, as previously explained. 20, 40 Data was interpreted with SCA 20 software (Dataphysics).
Surface energy was also calculated with the Owens, Wendt, Rabel and Kaelble (OWRK) equation applied to both water and diiodomethane measurements, as previously explained. 20, 40 Data was interpreted with SCA 20 software (Dataphysics).
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