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Human il 8 elisa ready set go 2nd generation kit

Manufactured by Thermo Fisher Scientific
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The Human IL-8 ELISA Ready-SET-Go! (2nd Generation) Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of interleukin-8 (IL-8) in human biological samples. The kit includes all the necessary components to perform the assay, including pre-coated plates, detection antibodies, and reagents.

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Lab products found in correlation

Flowcytomix pro 3, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions) Imark microplate absorbance reader, by Bio-Rad (1 mentions)

2 protocols using human il 8 elisa ready set go 2nd generation kit

1

Multiplex Cytokine Profiling in Serum

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For cytokine detection in serum samples, a Th1/Th2/Th9/Th17/Th22 13plex Kit FlowCytomix (eBioscience, Frankfurt, Germany) was used. This bead-based analyte detection system measured levels of IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, IL-22 and TNF-α. Samples were measured using the FACS Canto II (BD Biosciences). Data were analyzed with FlowCytomix Pro 3.0 software (eBioscience). In addition, IL-8 levels in serum samples were determined using the Human IL-8 ELISA Ready-SET-Go! (2nd Generation) Kit from eBioscience according to the manufacturer’s instructions.
Elfaki T.E., Arndts K., Wiszniewsky A., Ritter M., Goreish I.A., Atti El Mekki M.E., Arriens S., Pfarr K., Fimmers R., Doenhoff M., Hoerauf A, & Layland L.E. (2016). Multivariable Regression Analysis in Schistosoma mansoni-Infected Individuals in the Sudan Reveals Unique Immunoepidemiological Profiles in Uninfected, egg+ and Non-egg+ Infected Individuals. PLoS Neglected Tropical Diseases, 10(5), e0004629.
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2

Measurement of IL-8 by ELISA

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IL-8 production was measured using the Human IL-8 ELISA Ready-SET-Go! (2nd Generation) kit (eBioscience, Thermo Fisher Scientific, Waltham, MA) according to manufacturer's instructions. Wavelengths were measured with an iMark microplate absorbance reader (Bio-rad Laboratories, Hercules, CA).
Treffers L.W., van Houdt M., Bruggeman C.W., Heineke M.H., Zhao X.W., van der Heijden J., Nagelkerke S.Q., Verkuijlen P.J., Geissler J., Lissenberg-Thunnissen S., Valerius T., Peipp M., Franke K., van Bruggen R., Kuijpers T.W., van Egmond M., Vidarsson G., Matlung H.L, & van den Berg T.K. (2019). FcγRIIIb Restricts Antibody-Dependent Destruction of Cancer Cells by Human Neutrophils. Frontiers in Immunology, 9, 3124.
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