Gw3965 hydrochloride

Manufactured by Merck Group

Sourced in United States, Spain

GW3965 hydrochloride is a small molecule compound that functions as a selective agonist for the liver X receptor (LXR). It is commonly used in laboratory research settings to investigate the role of LXR in various biological processes.

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Lab products found in correlation

Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions) Phrodo green e coli bioparticles conjugate, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) Plasmid transfection medium, by Santa Cruz Biotechnology (1 mentions) Control crispr activation plasmid, by Santa Cruz Biotechnology (1 mentions) Live cell image solution, by Thermo Fisher Scientific (1 mentions) 7 ketocholesterol 5 cholesten 3β ol 7 one, by Merck Group (1 mentions) Ultracruz transfection reagent, by Santa Cruz Biotechnology (1 mentions) Dmem f 12 glutamax, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Ici 182 780, by Bio-Techne (1 mentions) Gw4064, by Merck Group (1 mentions) Gw0742, by Bio-Techne (1 mentions) 3 3 5 triiodo l thyronine sodium salt t3, by Merck Group (1 mentions) Wy14643, by Merck Group (1 mentions) Retinoic acid, by Merck Group (1 mentions)

3 protocols using gw3965 hydrochloride

Inducing Foam Cell Formation in Mouse Macrophages

Cited 1 time

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The mouse macrophage cell line was purchased from American Type Culture Collection. Cells were maintained as described previously. Mouse primary peritoneal macrophage was obtained as described[20 (link)]. Briefly, 4% thioglycollate was IP injected into mice. After 4 days, peritoneal macrophages were collected by peritoneal lavage. Cells were incubated with either 50ng/ml oxLDL or AcLDL (Intra Cel Resource, MD, USA) to induce foam cell formation. GW3965 hydrochloride (G6295, Sigma-Aldrich, MO, USA) at 5 μM was used for LXR agonist treatment [21 (link)].

Lin Y.W., Liu P.S., Adhikari N., Hall J.L, & Wei L.N. (2014). RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages. Journal of molecular and cellular cardiology, 79, 287-294.

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Adenoviral Mediated Knockdown of CPT1a in Cell Culture

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Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 with GlutaMAX (DMEM/F12 + GlutaMAX), Heat Inactivated Fetal Bovine Serum (FBS) and Penicillin/Streptomycin were obtained from Gibco (Madrid, Spain). Adenovirus vector with a short hairpin RNA target to CPT1a and the control non-targeted were generated by Galapagos NV (Mechelen, Belgium). Plasmid Transfection Medium, UltraCruz Transfection Reagent, Control CRISPR Activation Plasmid and CPT1 CRISPR Activation Plasmid were obtained from Santa Cruz Biotechnology, Inc. (Heidelberg, Germany). Live cell image solution and pHrodo Green E. coli BioParticles conjugate were purchased from Molecular Probes (Madrid, Spain). 7-Ketocholesterol (5-Cholesten-3β-ol-7-one) and GW3965 hydrochloride were obtained from Sigma-Aldrich (Madrid, Spain). C75 (4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid) was purchased from Santa Cruz Biotechnology, Inc. (Heidelberg, Germany). 7-ketocholesterol stock solution was prepared by dissolving the chemical compound in 100% ethanol at concentration of 5 mg/ml. C75 and GW3965 stock solutions were prepared in dimethyl sulfoxide (DMSO) at a concentration of 10 mg/ml and 32,34 mM respectively. Designed primers were bought from Life Technologies (Madrid, Spain).

Calle P., Muñoz A., Sola A, & Hotter G. (2019). CPT1a gene expression reverses the inflammatory and anti-phagocytic effect of 7-ketocholesterol in RAW264.7 macrophages. Lipids in Health and Disease, 18, 215.

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Receptor Activation Pathway Modulation

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17β-estradiol (E2, #E8875), LG100268 (#SML0279), HX531 (#SML2170), SR12813 (#S4194), 1α,25-Dihydroxyvitamin D3 (Vitamin D3, #D1530), Citco (#C6240), 3,3’,5-Triiodo-L-thyronine sodium salt (T3, #T6397), GW4064 (#G5172), GW3965 hydrochloride (#G6295), retinoic acid (#R2625), WY14643 (#C7081) were obtained from Sigma-Aldrich, and ICI 182,780 (Fulvestrant, #1047), GW0742 (#2229) was purchased from Tocris. For knockdown experiments, SMARTpool siRNAs against FOXA1 (#M-010319), RXRA (#L-003443) and RXRB (#L-003444) were obtained from Horizon Discovery. All siRNA sequences are indicated below:
FOXA1 siRNAs 1#: CCUCGGAGCAGCAGCAUAA; 2#: GCGCUGAGCCCGAGCGGCA; 3#: CGGGAAGACCGGCCAGCUA; 4#: GUGUAGACAUCCUCCGUAU.
RXRA siRNAs 1#: GCGCCAUCGUCCUCUUUAA; 2#: GCAAGGACCGGAACGAGAA; 3#: AGACCUACGUGGAGGCAAA; 4#: UCAAAUGCCUGGAACAUCU.
RXRB siRNAs 1#: GGGCAAUCAUUCUGUUUAA; 2#: GCAAACGGCUAUGUGCAAU; 3#: CGAAGAGGAUCCCACACUU; 4#: CCGCAAAGACCUUACAUAC.

Arcese P., Smith J.N, & Hatch M.I. (1996). Nest predation by cowbirds and its consequences for passerine demography.. Proceedings of the National Academy of Sciences of the United States of America, 93(10), 4608-4611.

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