Standard chromogenic reagents lead acetate, potassium dichromate, ferric chloride, hydrochloric acid, sulfuric acid, Mayer's reagent, Dragendorff's reagent, Wagner's reagent, Hager's reagent, Molisch reagent, Benedict's reagent, and Fehling's solutions used for preliminary phytochemical chemical group test were of reagent grade and purchased from Sigma-Aldrich Co. LLC, Missouri, United States. Vincristine sulfate, used as a standard drug in the cytotoxic assay, was collected from the Techno Drugs Limited, Bangladesh. Methanol supplied by Laboratory Patterson Scientific, UK, was used as solvent for maceration of the plant material. Dimethyl sulfoxide (DMSO, ≥99.9%, BioReagent, for molecular biology; Sigma-Aldrich, India) was used to dissolve the extracts.
Benedict s reagent
Manufactured by Merck Group
Sourced in Germany
Benedict's reagent is a laboratory chemical solution used as a qualitative test for the presence of reducing sugars. It is a clear, light blue liquid composed of copper sulfate, sodium carbonate, and sodium citrate in an aqueous solution. The core function of Benedict's reagent is to detect the presence of reducing sugars through a color change reaction.
Automatically generated - may contain errors
Lab products found in correlation
Sulfuric acid, by Merck Group (3 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Phenol solution, by Merck Group (2 mentions)
Sudan 3, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Glucose, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (2 mentions)
Lead acetate, by Merck Group (1 mentions)
Potassium dichromate, by Merck Group (1 mentions)
Dragendorff s reagent, by Merck Group (1 mentions)
Ferric chloride, by Merck Group (1 mentions)
Mayer s reagent, by Merck Group (1 mentions)
Molisch reagent, by Merck Group (1 mentions)
Bioreagent, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Ellman s reagent, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl dpph 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid, by Merck Group (1 mentions)
4 protocols using benedict s reagent
1
Phytochemical Screening and Cytotoxicity Assay
2
Flaxseed Oil Cake Characterization
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Flaxseed oil cake (FOC) was kindly donated by ACS Sp. z o.o. (Bydgoszcz, Poland) and cold-pressed flaxseed oil was obtained via a cold press technique (Olejarnia Niwki, Zwoleń, Poland). According to the manufacturer’s information, the proximate composition of FOC was as follows: 80.5% solids content, 42% protein content, 28% carbohydrates content, 6.3% fiber content, 6.1% fat content, 4.5% ash content. Sodium dodecyl sulphate (SDS), ethanol (96%), hydrochloric acid, Tween 80, Sudan III, sodium hydroxide, Benedict’s reagent, phenol solution (5%), sulfuric acid (96%), glucose, bovine serum albumin were purchased from Sigma Aldrich (Darmstadt, Germany). All reagents were of analytical grade.
3
Colorimetric Assay for Reducing Sugars
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Reaction products in film wash (20 mL) of either buffer or enzyme were concentrated to 2 mL by evaporation at 60 °C. Then, the solution was cooled down to room temperature. Then, 0.2 mL of Benedict’s reagent (Sigma Aldrich, Dorset, England) was added to the vial. The presence of the alkaline sodium carbonate in the reagent converted the reducing sugars into enediols, which in turn decrease the cupric particles (Cu2+) present in Benedict’s reagent to cuprous particles (Cu+); upon heating, these appear as red copper oxide (Cu2O), which is insoluble in water. The vial was then boiled for 3–4 min. Then, color change was qualitatively examined.
4
Characterization of Flaxseed Oil Cake
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Flaxseed oil cake (FOC) obtained via cold-press technique was kindly donated by ACS Sp. z o.o. (Bydgoszcz, Poland), and rapeseed oil (Zakłady Tłuszczowe, Kruszwica, Poland) was also used. According to the manufacturer’s information, the proximate composition of FOC was as follows: solid content 80.50%, ash content 4.50%, protein content 41.97%, fat content 6.11%, carbohydrates 27.99%, and fiber 6.29%. Sodium dodecyl sulphate (SDS), Sudan III, sodium hydroxide, Benedict’s reagent, phenol solution (5%), sulfuric acid (96%), glucose, bovine serum albumin, Ellman’s reagent (5,5′-dithiobis-2-nitrobenzoic acid), β-mercaptoethanol, trichloroacetic acid, urea, glycine, ethylenediaminetetraacetic acid (EDTA), tris(hydroxymethyl)aminomethane (Tris), ethanol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and methanol were purchased from Sigma Aldrich (Darmstadt, Germany). All reagents were of analytical grade.
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