Anti cd27 lg7f9

Manufactured by Thermo Fisher Scientific

The Anti-CD27 (LG7F9) is a lab equipment product that functions as an antibody specific to the CD27 receptor. CD27 is a member of the tumor necrosis factor receptor superfamily and is involved in the regulation of immune responses. The Anti-CD27 (LG7F9) product can be used for the detection and analysis of CD27-expressing cells in various research applications.

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2 protocols using anti cd27 lg7f9

Multiparametric Flow Cytometry Analysis

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The harvested spleens were mashed and single-cell suspensions of the spleens were stained with the following surface antibodies: anti-CD45 (30-F11, BD Biosciences), LIVE/DEAD Fixable Blue cell stain (Invitrogen), anti-CD19 (ID3, BD Biosciences), anti-CD8a (53-6.7, BioLegend), anti-CD27 (LG7F9, eBioscience), anti-CD11b (M1/70, BioLegend), anti-CD11c (HL3, BD Biosciences), anti-CD86 (GL1, BioLegend), anti-NK1.1 (PK136, BioLegend), anti-NKp46 (29A1.4, BioLegend), anti-MHC Class II (M5/114.15.2, BD Biosciences), anti-F4/80 (BM8, BioLegend), anti-CD80 (16-10A1), anti-CD3 (17A2, BioLegend), anti-CD4 (RM4-5, Invitrogen), anti-CTLA-4 (UC10-4B9, BioLegend), anti-PD-1 (29F.1A12, eBioscience), anti-CD28 (37.51, BioLegend), anti-CD44 (IM7, BD Biosciences), anti-CD43 (1B11, BioLegend), anti-CD47 (miap301, BioLegend), anti-CD62L (MEL-14, BioLegend), anti-CD25 (PC61.5, eBioscience), and anti-CD107a (1D4B, BioLegend). Intracellular staining was then performed with a FOXP3 permeabilization and fixation kit (eBioscience) according to manufacturer’s recommendations using the following antibodies: Ki-67 (B56; BD Biosciences) and GrB (QA16A02, BioLegend). Flow cytometric data were collected with a Beckman Coulter Cytoflex LX (6-L NUV) flow cytometer and analyzed using FlowJo software (Tree Star).

Rao D., O’Donnell K.L., Carmody A., Weissman I.L., Hasenkrug K.J, & Marzi A. (2021). CD47 expression attenuates Ebola virus-induced immunopathology in mice. Antiviral research, 197, 105226.

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Multicolor Flow Cytometry Panel

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The antibodies used for analysis for flow cytometry were directed against the following proteins: CD3ε (145.2C.11), CD4 (RM4-5), CD8α (53.6.7), CD11b (M1/70), CD19 (1D3), CD44 (1M7), CD49b (DX5), CD69 (H1-2F3), CD107a (1D4B), H-2 IA/E (M5/114.15.2), IFN-γ (XMG1.2), mouse IgG1 (A851), Ly6C (AL21) and Fluorochrome-conjugated Streptavidin were obtained from BD BioScience (San Diego, CA). Anti-CD27 (LG7F9) and Siglec H (440c) were obtained from eBioScience (San Diego, CA). Anti-CD11c (N418) was obtained from BioLegend (San Diego, CA). Anti-human IgG (Fc part)-Biotin by Jackson ImmunoResearch Laboratories (West Grove, PA). Anti-Ly49H Ab 3D10 producing hybridoma was kindly provided by Prof. W.M. Yokoyama (Washington University, St. Louis, MO) and anti-Ly49C Ab 4LO3311 producing hybridoma was kindly provided by Prof. S. Lemieux (INRS, Laval, Canada). Purified anti-Ly49C and Ly49H antibodies were prepared in-house and conjugated to fluorochrome using antibody-labeling kit according to the manufacturer's recommendations (Molecular Probes).

Forbes C.A., Scalzo A.A., Degli-Esposti M.A, & Coudert J.D. (2014). Ly49C-Dependent Control of MCMV Infection by NK Cells Is Cis-Regulated by MHC Class I Molecules. PLoS Pathogens, 10(5), e1004161.

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