1260 infinity gpc apparatus

Gel permeation chromatography (GPC) was performed to evaluated molecular weight (Mw) and molecular number (Mn) of PLA:PCL SMPNs after gamma irradiation.
Analyses were performed using Agilent Technologies 1260 Infinity GPC apparatus, a chromatographic system composed by a precolumn (Agilent GPC/SEC Guard Columns) and three Ultrastyragel columns connected in series (7.7 × 250 mm each with different pore diameters of 104 Å, 103 Å, and 500 Å), a pump (Agilent Technologies 1260 Infinity), an IR detector (Agilent Technologies 1260 Infinity), and a data handling software (OpenLab and Cirrus).
PLA:PCL pristine powder and non-irradiated and gamma-irradiated PLA:PCL electrospun nanofibrous scaffolds were solubilized in THF and the solutions obtained (1 mg/mL) were filtered with a Fluoropore 0.45-μm (Millipore) filter before being injected in GPC; THF was the mobile phase and constant flow rate was set at 1 mL/min.

PLA–PCL molecular weight determination was performed by 1260 Infinity GPC apparatus (Agilent Technologies, Santa Clara, CA, USA) equipped with three different PhenoGEL columns (5-µm particle size—500-Å, 103-Å, 104-Å pore size) and a refractive index detector. THF was used as mobile phase and flow rate was set at 1 mL/min.
Electrospun matrices were dissolved in THF to reach the final PLA–PCL concentration of 3 mg/mL, GNPs was removed by centrifugation and the polymer solutions were filtered throughout 0.45 μm nylon filters and analyzed. PLA–PCL molecular number, molecular weight and polydispersity index were determined against polystyrene standards whose molecular weight ranged from 1480 and 361,500 Da. Neat PLA–PCL mats were used as control.