B subtilis pgn

Manufactured by InvivoGen

Sourced in United States

B. subtilis PGN is a bacterial peptidoglycan preparation derived from Bacillus subtilis. It is a structural component of the Gram-positive bacterial cell wall.

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Lab products found in correlation

Mutanolysin, by Merck Group (1 mentions)

Spelling variants of this product

PGN-BS (4 citations) B. subtilis (2 citations)

2 protocols using b subtilis pgn

Purification and Preparation of PGN and LPS

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Flg22 peptide has been described previously (Felix et al., 1999 (link)). The purification of P. syringae pv. tomato PGN was performed as described previously (Willmann et al., 2011 (link)). M. luteus cell wall preparations and B. subtilis PGN were purchased from Invivogen (San Diego, California, United States), Cecolabs (Tübingen, Germany), and Sigma-Aldrich (Hamburg, Germany). PGNs and LPS (from P. aeruginosa, Sigma-Aldrich) were dissolved in water at a concentration of 10 mg/ml and stored at −20°C. Mutanolysin was purchased from Sigma-Aldrich.

Liu X., Grabherr H.M., Willmann R., Kolb D., Brunner F., Bertsche U., Kühner D., Franz-Wachtel M., Amin B., Felix G., Ongena M., Nürnberger T, & Gust A.A. (2014). Host-induced bacterial cell wall decomposition mediates pattern-triggered immunity in Arabidopsis. eLife, 3, e01990.

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PGN Treatment Modulates Murine Immunity

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Female C57BL/6 and MRL/lpr mice (Jackson Laboratories, Bar Harbor, ME, USA) were housed at the Medical University of South Carolina vivarium (MUSC). Mice were i.p. injected with PBS, S. aureus PGN, or B. subtilis PGN (100 μg/time) (InvivoGen, San Diego, CA). C57BL/6 mice (6-week-old) were treated with PGNs and PBS twice per week for 8 weeks; then the treatment was stopped for another 8 weeks. MRL/lpr lupus-prone mice (6-week-old) were treated with PBS and PGNs twice per week for 12 weeks.

Ning W., Cheng D., Howe P.H., Bian C., Kamen D.L., Luo Z., Fu X., Ogunrinde E., Yang L., Wang X., Li Q.Z., Oates J., Zhang W., White D., Wan Z., Gilkeson G.S, & Jiang W. (2022). Staphylococcus aureus peptidoglycan (PGN) induces pathogenic autoantibody production via autoreactive B cell receptor clonal selection, implications in systemic lupus erythematosus. Journal of autoimmunity, 131, 102860.

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