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Anti entactin nidogen

Manufactured by Abcam
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Anti-entactin/nidogen is a lab equipment product used in research applications. It functions as an antibody that specifically binds to the entactin/nidogen protein, which is a component of the extracellular matrix.

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Lab products found in correlation

Anti ln 332, by Abcam (2 mentions) Anti lnγ2l4m, by Abcam (2 mentions) Alexa fluor 594 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Anti cytokeratin 14, by Abcam (2 mentions)

2 protocols using anti entactin nidogen

1

Immunofluorescence Staining of Basement Membrane Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen sections (10μM) were rehydrated in PBS with 0.2% Tween-20 for 10 minutes, blocked in 10% heat-inactivated goat serum and 5% milk in PBS for 1 hour, then stained with the following rabbit polyclonal antisera: anti-α3 integrin or corresponding pre-immune serum (1:100)(DiPersio et al., 1995 (link)); anti-LN-332 (1:200; Abcam, Cambridge, MA); anti-entactin/nidogen (1:1000; Abcam); anti-LNγ2L4m (1:1000)(Sasaki et al., 2001 (link)); anti-fibulin-2 (1:2000)(Pan et al., 1993 (link)). Sections were co-immunostained in some cases with mouse monoclonal anti-cytokeratin 14 (1:500; Abcam). Secondary antibodies were fluorescein-conjugated goat anti-mouse IgG (1:250; Pierce, Rockford, IL) or Alexa Fluor 594 goat anti-rabbit IgG (1:250; Molecular Probes, Eugene, OR), as appropriate. Images were collected on a Nikon Eclipse 80i using a Spot camera (Diagnostic Instruments, Sterling Heights, MI).
Longmate W.M., Monichan R., Chu M.L., Tsuda T., Mahoney M.G, & DiPersio C.M. (2014). Reduced fibulin-2 contributes to loss of basement membrane integrity and skin blistering in mice lacking integrin α3β1 in the epidermis. The Journal of investigative dermatology, 134(6), 1609-1617.
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2

Immunofluorescence Staining of Basement Membrane Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen sections (10μM) were rehydrated in PBS with 0.2% Tween-20 for 10 minutes, blocked in 10% heat-inactivated goat serum and 5% milk in PBS for 1 hour, then stained with the following rabbit polyclonal antisera: anti-α3 integrin or corresponding pre-immune serum (1:100)(DiPersio et al., 1995 (link)); anti-LN-332 (1:200; Abcam, Cambridge, MA); anti-entactin/nidogen (1:1000; Abcam); anti-LNγ2L4m (1:1000)(Sasaki et al., 2001 (link)); anti-fibulin-2 (1:2000)(Pan et al., 1993 (link)). Sections were co-immunostained in some cases with mouse monoclonal anti-cytokeratin 14 (1:500; Abcam). Secondary antibodies were fluorescein-conjugated goat anti-mouse IgG (1:250; Pierce, Rockford, IL) or Alexa Fluor 594 goat anti-rabbit IgG (1:250; Molecular Probes, Eugene, OR), as appropriate. Images were collected on a Nikon Eclipse 80i using a Spot camera (Diagnostic Instruments, Sterling Heights, MI).
Longmate W.M., Monichan R., Chu M.L., Tsuda T., Mahoney M.G, & DiPersio C.M. (2014). Reduced fibulin-2 contributes to loss of basement membrane integrity and skin blistering in mice lacking integrin α3β1 in the epidermis. The Journal of investigative dermatology, 134(6), 1609-1617.
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