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89 protocols using benzoyl peroxide

1

Fibrinogen-Conjugated Nanoparticle Synthesis

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Alexa Fluor 488 – conjugated fibrinogen was purchased from Invitrogen. Human fibrinogen, thrombin and gold nanorods (GNRs) (axial diameter: 9.0–11.0 nm; longitudinal diameter: 36.9–45.1 nm), methyl methacrylate (MMA), butylmethacrylate (BMA), methylbenzoate, polyethylene glycol 400, benzoyl peroxide and N,N-dimethyl-p-toluidine were procured from Sigma, while methylene blue, benzoyl peroxide and Drabkin's solution were products of Merck, Thomas baker and Linear Chemicals S.L.U., respectively. The rest of the chemicals were either from Sigma or Merck. All reagents were of analytical grade. Type I deionized water (18.2 MΩ cm, Millipore) has been used throughout the experiment.
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2

Kaempferia galanga Rhizome Extract Characterization

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Kaempferia galanga L. rhizome extract was provided by Herbal Study Center, Faculty of Pharmacy (Universitas Padjadjaran). Ethyl p-methoxycinnamate (EPMC), cinnamaldehyde (CD), and kaempferol (KF) were purchased from MarkHerb (Bandung, Indonesia). Benzoyl peroxide (BPO), 2-hydroxyethyl methacrylate (HEMA), methacrylic acid (MAA), Benzoyl peroxide (BPO), ethyl cinnamate (EC), methyl methacrylate (MMA), and trifluoroAcetic acid (TFA) were purchased from Sigma-Aldrich (Missouri, USA). 2-(Trifluoromethyl)acrylic acid (TFMAA), ethylene glycol dimethacrylate (EGDMA), and trimethylol propane trimethacrylate (TRIM) were purchased from Tokyo Chemical Industry (Tokyo, Japan). Acetic acid, ethanol, ethyl acetate, HPLC-grade acetonitrile, isopropanol, and n-hexane were purchased from Merck (Darmstadt, Germany). All materials other than those described were of pro analysis grades.
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Comprehensive Bone Histomorphometry Analysis

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Static and dynamic histomorphometry was performed on decalcified and undecalcified femoral sections of mice.
For static analysis, femurs or tibias were fixed in 4% paraformaldehyde (Merck) for 3 days, washed for 2 hours with water, and decalcified in the presence of 15% EDTA for 10 days with mild shaking at 37°C. The bones were embedded in paraffin. Osteoclast number/bone perimeter and osteoclast surface/bone surface were quantified on decalcified sections of either tibia or femur stained for tartrate‐resistant acid phosphatase (TRAP).
Undecalcified femurs were first dehydrated and infiltrated with destabilized methylmetacrylate (Merck), benzoylperoxide (Merck), and nonylphenyl‐polyethyleneglycol acetate (Sigma‐Aldrich, St. Louis, MO, USA) for 14 days. Embedding in methylmetracrylate was done overnight at 4°C, and the femurs were used for dynamic analysis. Osteoblast number/bone perimeter, osteoblast surface/bone surface, and osteocyte number were quantified on toluidine blue‐stained undecalcified femoral sections. Two sections were analyzed for each mouse. Bone formation rate (BFR)/bone surface was measured on undecalcified bone sections by means of fluorochrome labeling. Osteomeasure software was used for the analysis (Osteometrics, Decatur, GA, USA)
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4

Wood Modification with Chemicals

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Sindora glabra wood was obtained from Forest Farm, Sarawak, Malaysia. All the wood samples were cut to dimensions of 30 cm x 2 cm x 1 cm. To ensure the wood was well modified, the chemicals namely FA, EHMA, benzoyl peroxide and halloysite nanoclay (HNC) used. HNC was supplied by Sigma Aldrich (USA) with a diameter of 30 to 70 nm and length was in between 1 and 3 μm. The chemicals FA, EHMA and benzoyl peroxide, all these chemicals were supplied by Merck Millipore (USA).
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5

Dimerization of Hex-1-ene using Zirconium Complex

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TIBA (1 M solution in hexane, Merck, NJ, USA), MMAO-12 (1.52 M solution in Toluene, Merck, NJ, USA), azobisisobutyronitrile (AIBN, Merck, NJ, USA), benzoyl peroxide (Merck, NJ, USA), mesitylene (Merck, NJ, USA) and CDCl3 (99.8% 2H, Cambridge Isotope Laboratories, Inc., MS, USA) were used as purchased. Toluene (Merck, NJ, USA) was refluxed over sodium and stored under argon atmosphere. Maleic anhydride (MA, Merck, NJ, USA) was recrystallized from Toluene before use. Hex-1-ene (Merck, NJ, USA) was stored over Na wire and distilled under argon. The 1H NMR spectra were recorded on a Bruker AVANCE 400 spectrometer (400 MHz, Bruker, MS, USA) at 20 °C. The chemical shifts are reported in ppm relative to the solvent residual peaks.
Zirconium complex 1 was prepared in accordance with published procedure [81 (link)]. Dimerization of hex-1-ene was conducted in liquid olefin media in the presence of 1, activated by TIBA and MMAO-12 using the protocol described previously [47 (link),49 (link)], 5-methyleneundecane was separated by the vacuum distillation, b. p. 80 °C at 7 Torr.
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6

Synthesis of D-mannose Oligomers

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D-mannose based oligomer (M) was isolated in our laboratory previously [17 ]. 2-hydroxypropyl acrylate (HPA) and benzoyl peroxide (BOP) were purchased from Merck (Merck KGaA, Darmstadt, Germany) and used without purification. All other reagents were used as purchased.
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7

Synthesis and Functionalization of PS/DVB Particles

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The PS/DVB particles used as supporting material for all functionalizations were prepared according to the procedure described in Refs. [47 (link),48 ] for materials with 55% nominal crosslinkage. The used polymer batches had average diameters between 4.0 and 4.5 μm and were highly monodisperse with specific surface areas AS of 1000–1200 m2 g−1 and mean pore sizes Φ50 of 40–45 nm.
Ultrapure water was used as received from a MilliQ-system (Merck-Millipore Billerica, MA, USA). The monomer was synthesized using 4-vinylbenzyl chloride (90%, Sigma-Aldrich, Steinheim, Germany) and N-methyl diethanolamine (≥99% Sigma-Aldrich, Steinheim, Germany) without further treatment. The detailed procedure is given in Ref. [46 (link)]. DMSO (99.5%, Grüssing GmbH, Grüssing, Germany) and benzoyl peroxide (for synthesis, 25% water, Merck, Darmstadt, Germany) as well as the organic solvents for the synthesis were used as received.
For preparation of the eluents sodium carbonate (p.a., VWR chemicals, Leuven, Belgium) and sodium hydroxide solution (50%, p.a., Merck, Darmstadt, Germany) were used. The IC standards were prepared from the sodium salts (p.a.) of the anions.
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Synthesis of Nanocomposite Dental Materials

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Nano-titanium oxide, ammonium chloride (NH₄Cl, 99%), resorcinol (C₆H₆O₂, 99%), sodium hydroxide (NaOH, 98%), urea (CH₄N₂O, 99%), poly(ethylene-alt-maleic anhydride) (EMA), N,N-dihydroxyethyl p-toluidine (DEPT, 90%)), and benzoyl peroxide (BPO, 98%) were obtained from Merck. Formaldehyde solution (37%), bisphenol A-glycidyl methacrylate (Bis-GMA, 97%), triethylene glycol dimethacrylate (TEGDMA, 95%), camphorquinone (C10H14O2, 97%), urethane dimethacrylate (UDMA, 97%), nanoclay, and nano-chitosan were purchased from Sigma-Aldrich.
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Comprehensive Chemical Reagents Procurement

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Tetracycline, erythromycin, benzoyl peroxide, crystal violet, actinomycin D, dimethyl sulfoxide (DMSO), Tris, KCl, 5,5’-dithio-bis-(2-nitrobenzoic acid) (DTNB), DMPTB, Triton X-100, ibuprofen, porcine hematin, L-epinephrine, formic acid, Na2EDTA, Griess reagent, citric acid, Na2HPO4, NaCl, BSA, NaOH, and CTAB were purchased from Merck SA (Pty) Ltd. (Sandton, Johannesburg, SA). PrestoBlue was purchased from Thermo Fisher Scientific (Randburg, South Africa). TRIzol and molecular grade water were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA). Dexamethasone was purchased from Sigma Chemicals Co. (St. Louis, Missouri, USA). Ultra pure liquid chromatography water and acetonitrile (Romil-UpS) were purchased from Microsep, South Africa. formic acid (99% purity) was purchased from Thermo Scientific, South Africa. Fluka® Analytical Ammonium hydroxide ≥ 25% in H2O, eluent additive for liquid chromatography (LC) was purchased from Sigma-Aldrich, South Africa.
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10

Synthesis of Organic Compounds: A Comprehensive Approach

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Copper
nitrate trihydrate
(Merck-AR), sodium hydroxide (Merck-LR), poly(acrylic acid) (weight
average molecular weight MW ∼240000
by GPC, 25 wt % in H2O) (Sigma-Aldrich-AR), l-ascorbic
acid (S.D. Fine Chemicals-LR), sodium ascorbate (Merck-LR), propargyl
bromide (Merck-LR), 2-aminophenol (Merck-LR), 2-aminothiophenol (Merck-LR),
4-methylcinnamic acid (Merck-LR), 4-methyl benzaldehyde (Merck-LR),
phosphorus oxychloride (Merck-LR), pyrocatechol (Merck-LR), acetylene
(National Oxygen Limited), N-bromosuccinimide (NBS)
(S.D. Fine Chemicals-LR), benzoyl peroxide (Merck-LR), potassium carbonate
(Merck-LR), sodium bicarbonate (Merck-LR), sodium sulfate (Merck-LR),
dimethyl sulfoxide (DMSO( (Merck-AR), dimethylformamide (DMF) (Merck-AR),
tetrahydrofuran (THF) (Merck-LR), hexane (Merck-LR), acetone (S.D.
Fine Chemicals-AR), chloroform (CHCl3) (S.D. Fine Chemicals-AR),
tetrachloromethane (S.D. Fine Chemicals-AR), methanol (S.D. Fine Chemicals-AR),
ethanol (S.D. Fine Chemicals-AR), and double distilled water were
used.
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