Ab131390
Manufactured by Abcam
Sourced in United States
Ab131390 is an antibody that recognizes a specific target in biological samples. The product is suitable for use in various laboratory applications, but a detailed description of its function or intended use is not available.
Automatically generated - may contain errors
Lab products found in correlation
Ab8226, by Abcam (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Af788, by R&D Systems (1 mentions)
A32731, by Thermo Fisher Scientific (1 mentions)
Confocal laser scanning microscope, by Olympus (1 mentions)
Image pro plus 6, by Media Cybernetics (1 mentions)
Ab104224, by Abcam (1 mentions)
C0060, by Solarbio (1 mentions)
Ab56416, by Abcam (1 mentions)
Ab194676, by Abcam (1 mentions)
Ab48394, by Abcam (1 mentions)
5 protocols using ab131390
1
Quantifying Hippocampal Fndc5/Irisin Levels
2
Protein Expression Analysis of Muscle Markers
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RIPA lysis buffer supplemented with a complete protease inhibitor cocktail was used to lyse cells and tissues on ice. Equal amounts of protein (10 µg) were separated on a 10% SDS-PAGE gel. Then, the proteins were was transferred to PVDF membranes (0.22 µm, Millipore). Membranes were blocked in 5% skim milk and incubated with primary antibodies against MSTN (AF788; R&D Systems), FNDC5 (ab131390; Abcam), MYH4 (20140-1-AP, Proteintech) and Tubulin (11224-1-AP, Proteintech) was used as CTR.
3
Immunocytochemistry of Irisin in BMMSCs
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Following 7 days of osteogenic induction, the BMMSCs were fixed with 4% paraformaldehyde for 10 min. After blocking with 5% BSA for 30 min, the cells were incubated with primary antibody (anti-irisin antibody (1:500 dilution, ab131390; Abcam) overnight at 4°C. Then, the cells were incubated with secondary antibodies conjugated with anti-rabbit Alexa Fluor 488 secondary antibody (1:200 dilution; A32731; Invitrogen) for 1 h at 37°C. Finally, the BMMSCs were stained with DAPI for 10 min and imaged under a confocal laser scanning microscope (Olympus) (38 (link)).
4
Double Immunofluorescence Staining of Irisin and NeuN
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Double fluorescence staining was performed as previously described (Pang et al., 2018 (link)). The slices were rewarmed at room temperature for 10 min and permeabilized with 0.1% Triton X-100 (diluted in PBS) for 5 min. After being washed with PBS three times (5 min per time), the slices were blocked with 10% goat serum (diluted in PBS) for 2 h at room temperature. Then, the slices were incubated overnight (about 12 h) at 4°C with primary antibodies, including anti-irisin (ab131390, Abcam, USA, 1:100) and anti-NeuN (ab104224, Abcam, USA, 1:100).
Next, the slices were washed with PBS and incubated with the appropriate secondary antibody (1:200) at room temperature for 2 h. Finally, 4',6-diamidino-2-phenylindole (DAPI; C0060, Solarbio, China, 1:200) were used to stain the cell nucleus for 5 min at room temperature. After dropping the anti-fluorescence attenuation agents (20 μl per tissue) and covering the cover slides, the slices were observed and recorded with a fluorescence microscope. Next, three random coronal sections per brain were selected, and the defined regions around the puncture point were observed in the right hemisphere for analysis. Photographs and fluorescence intensity were analyzed with Image-Pro Plus 6.0 software (MediaCybernetics, USA).
Next, the slices were washed with PBS and incubated with the appropriate secondary antibody (1:200) at room temperature for 2 h. Finally, 4',6-diamidino-2-phenylindole (DAPI; C0060, Solarbio, China, 1:200) were used to stain the cell nucleus for 5 min at room temperature. After dropping the anti-fluorescence attenuation agents (20 μl per tissue) and covering the cover slides, the slices were observed and recorded with a fluorescence microscope. Next, three random coronal sections per brain were selected, and the defined regions around the puncture point were observed in the right hemisphere for analysis. Photographs and fluorescence intensity were analyzed with Image-Pro Plus 6.0 software (MediaCybernetics, USA).
5
Western Blot Analysis of Muscle Proteins
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Gastrocnemius muscle protein was extracted and mixed with 5× loading buffer (Applygen, Beijing, China). The protein was then denatured at 100 ° C for 5 min and then loaded on a SDS-PAGE gel prior to being transferred to NC membranes. Following the immersion of membranes with 5% BSA (AMRESCO Inc., OH, USA), membranes were incubated with primary rabbit polyclonal anti-UII antibody (ab194676, Abcam), primary rabbit polyclonal anti-LC3 antibody (ab48394, Abcam), primary mouse monoclonal anti-p62 antibody (ab56416, Abcam), and primary rabbit polyclonal anti-FNDC5 antibody (ab131390, Abcam) overnight at 4 ° C. Membranes were then incubated with fluorescence-conjugated antirabbit and antimouse antibodies (LI-COR, NE, USA). Semi-quantitative gray-scale intensity was measured by image J. The antibody of GAPDH was purchased from Byeotime (Shanghai, China).
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