Log In Sign up
The largest database of trusted experimental protocols

Atg101

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

ATG101 is a lab equipment product manufactured by Cell Signaling Technology. It is an antibody that recognizes the autophagy-related protein 101 (ATG101), which is a component of the autophagy initiation complex involved in the regulation of autophagy, a cellular process that recycles damaged or unnecessary components.

Automatically generated - may contain errors

Lab products found in correlation

Fip200, by Cell Signaling Technology (4 mentions) Beclin 1, by Cell Signaling Technology (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Antifade gold mounting medium, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Pvdf membrane, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hydrogen peroxide, by Thermo Fisher Scientific (2 mentions) Dmem without glucose, by Thermo Fisher Scientific (2 mentions) Bafilomycin, by Thermo Fisher Scientific (2 mentions) Dmso cell culturegrade, by Thermo Fisher Scientific (2 mentions) Antibodies for actin, by Cell Signaling Technology (2 mentions) Vps34, by Cell Signaling Technology (2 mentions) Ulk ser 317, by Cell Signaling Technology (2 mentions) Ulk ser 757, by Cell Signaling Technology (2 mentions) Atg13, by Cell Signaling Technology (2 mentions) Atg 13pser 318, by Cell Signaling Technology (2 mentions) Beclin ser 15, by Cell Signaling Technology (2 mentions)

4 protocols using atg101

1

Autophagy Regulatory Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hydrogen peroxide, bafilomycin, antifade gold mounting medium, FBS,
L-glutamine, penicillin /streptomycin, DMEM without glucose, DMEM without
sodium pyruvate for H2O2 treatment medium, PVDF membrane, DMSO cell culture
grade, Lipofectamine 2000, Lipofectamine 3000 were purchased from Thermo
Fisher Scientific. Polyfect was purchased from qiagen. Antibodies for actin,
LC3, ATG5, myc, VPS34, ULK ser 317, ULK ser 757, ATG 13, ATG 13pSer 318,
Beclin1, Beclin Ser 15, VPS34 Ser 249, AMPK, PAMPK Thr 172,Flag, GST,
HA,FIP200, ATG101 were purchased from Cell Signaling Technology. ULK1ser555
and ULK ser 777 were from Millipore. ULK1 for immunoprecipitation was
purchased from Sigma. ULK1 for western blots was purchased from Santacruz
Biotechnology. Anti-mouse IPMK was developed in our lab.
Guha P., Tyagi R., Chowdhury S., Reilly L., Fu C., Xu R., Resnick A.C, & Snyder S.H. (2019). IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration. Cell reports, 26(10), 2692-2703.e7.
+ Open protocol
+ Expand
2

Protein Extraction, Separation, and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins extraction from cells, separation by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE), blotting, and detection were performed as previously described.13 Primary antibodies against p70S6K (#9208), p‐p70S6K‐Thr389 (#9234), p‐S6‐Ser235/236 (#4858), S6 (#2217), LC3 (#12741), p62 (#8025), FIP200 (#12436), ULK1 (#8054), ATG7 (#8558), ATG5 (#12994), ATG12 (#4180), BECLIN (#3495), ATG3 (#3415), ATG101 (#13492), NIX (#12396), and β‐actin (#8457) were purchased from Cell Signaling Technology. Anti‐p27(F‐8) antibody was purchased from Santa Cruz Biotechnology (Dallas, Texas), E2F‐2 (ab138515) antibody was purchased from Abcam (Cambridge, UK). HRP‐linked anti‐rabbit and anti‐Mouse IgG were used at 1:5000 (#7074, #7076, Cell Signaling Technology). Band signal intensities were quantified as previously described.13
Liu Q., Luo L., Ren C., Zou M., Yang S., Cai B., Wu L., Wang Y., Fu S., Hua X., Tang N., Huang S., Huang X., Xin W., Chen F, & Zhang X. (2020). The opposing roles of the mTOR signaling pathway in different phases of human umbilical cord blood‐derived CD34 + cell erythropoiesis. Stem Cells (Dayton, Ohio), 38(11), 1492-1505.
+ Open protocol
+ Expand
3

Autophagy and Apoptosis Modulation by PVT in Pancreatic Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was performed in whole cell lysates of AsPC1, BxPC3, L3.6pl, MIAPaCa2, and PO2 cells treated with 2.5, 5, and 7.5 µM PVT for 24 h SDS-PAGE resolved proteins were immunoblotted for ULK1, pULK1(S757), Atg101, FIP200, Beclin-1, Atg-5, LC3A/B, cleaved caspase-3 and β-actin (Cell Signaling Technologies, Danvers, MA, USA). All experiments were repeated for at least 2 times.
Ramachandran S., Kaushik I.S, & Srivastava S.K. (2021). Pimavanserin: A Novel Autophagy Modulator for Pancreatic Cancer Treatment. Cancers, 13(22), 5661.
+ Open protocol
+ Expand
4

Autophagy Regulatory Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hydrogen peroxide, bafilomycin, antifade gold mounting medium, FBS,
L-glutamine, penicillin /streptomycin, DMEM without glucose, DMEM without
sodium pyruvate for H2O2 treatment medium, PVDF membrane, DMSO cell culture
grade, Lipofectamine 2000, Lipofectamine 3000 were purchased from Thermo
Fisher Scientific. Polyfect was purchased from qiagen. Antibodies for actin,
LC3, ATG5, myc, VPS34, ULK ser 317, ULK ser 757, ATG 13, ATG 13pSer 318,
Beclin1, Beclin Ser 15, VPS34 Ser 249, AMPK, PAMPK Thr 172,Flag, GST,
HA,FIP200, ATG101 were purchased from Cell Signaling Technology. ULK1ser555
and ULK ser 777 were from Millipore. ULK1 for immunoprecipitation was
purchased from Sigma. ULK1 for western blots was purchased from Santacruz
Biotechnology. Anti-mouse IPMK was developed in our lab.
Guha P., Tyagi R., Chowdhury S., Reilly L., Fu C., Xu R., Resnick A.C, & Snyder S.H. (2019). IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration. Cell reports, 26(10), 2692-2703.e7.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!