The Y-maze apparatus consisted of 3 arms, 32 cm (long) 610 cm (wide) with 26-cm walls (San Diego Instruments, San Diego, CA). The test was performed as previously described in details in our publications39 (link),40 (link).
Y maze apparatus
Manufactured by San Diego Instruments
Sourced in United States
The Y-maze apparatus is a laboratory equipment used for behavioral analysis. It consists of a Y-shaped maze with three distinct arms. The core function of the Y-maze apparatus is to assess spatial working memory and spontaneous alternation behavior in small animals, such as rodents.
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19 protocols using y maze apparatus
1
Y-Maze Apparatus for Spatial Memory
2
Spontaneous Alternation Y-Maze Test
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To allow the mice to discriminate between the three arms of the Y-maze apparatus (San Diego Instruments), the sides of one arm of was lined with a pattern of black vertical bars on a white background with a black square at the end. The sides of another arm were lined with a pattern of solid circles with black triangles on a white background and a triangle marking the end. The remaining arm was not marked. The Y-maze test was conducted for 10 min. The first minute was not coded and treated as the habituation period. Subsequently, the entries into each arm were recoded. The spontaneous alternation index was calculated as the number of non-repeating triplets (for example, if each arm was labeled “A”, “B” or “C”, “ABCAC” = 2) divided by the total number of possible non-repeating triplets (total number of entries into each arm - 2).
3
Y-Maze Apparatus for Behavioral Analysis
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The Y‐maze apparatus consisted of 3 arms 32cm long × 10cm wide with 26cm walls (San Diego Instruments, San Diego, CA). Testing was always performed in the same room and at the same time to ensure environmental consistency, as previously described [14 (link), 15 (link)].
4
Y‐maze Spontaneous Alternation Assay
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The Y‐maze apparatus consisted of 3 arms, 32 cm (long) 610 cm (wide) with 26‐cm walls (San Diego Instruments, San Diego, CA). As previously described13 , 14 , testing was always performed in the same room and at the same time to ensure environmental consistency as previously described. After introduction to the center of the Y‐maze, the animal is allowed to freely explore the three arms for 5 min and the sequence and total number of arms entered was video‐recorded in order to calculate the percentage of alternation. Any entry into an arm was considered valid if all limbs were within the arm. An alternation was defined as three consecutive entries in three different arms (1,2,3 or 2,3,1, etc.). The percentage alternation score was calculated using the following formula: total alternation number/total number of entries−2) × 100.
5
Y-Maze Spontaneous Alternation Behavior
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The Y-maze apparatus consisted of three arms 32 cm (long) 610 cm (wide) with 26-cm walls (San Diego Instruments, San Diego, CA) and testing was performed as previously described [17 (link)–19 ]. Briefly, each mouse was placed in the center of the Y-maze and allowed to explore freely for five minutes to measure spontaneous alternating behavior. The sequence and total number of entries were video-recorded. An entry into an arm was considered valid if all four paws entered the arm. An alternation was defined as three consecutive entries into three different arms (1, 2, 3, or 2, 3, 1, etc.). Percentage of alternation was calculated using the following formula: total alternation number/total number of entries−2) × 100.
6
Spontaneous Alternation Behavior Assessment
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The Y-maze apparatus consisted of 3 arms 32 cm (long) 610 cm (wide) with 26 cm walls (San Diego Instruments, San Diego, CA, USA). Testing was always performed in the same room and at the same time of the day to ensure environmental consistency, as previously described.12 (link) Briefly, each mouse was placed in the center of the Y-maze and allowed to explore freely through the maze during a 5-min session for the assessment of spontaneous alternating behavior. The sequence and total number of arms entered were video-recorded. Any entry into an arm was considered valid if all four paws entered the arm. An alternation was defined as three consecutive entries in three different arms (1,2,3 or 2,3,1 and so on). The percentage alternation score was calculated using the following formula: total alternation number/total number of entries−2) × 100.
7
Y-Maze Behavioral Assay in Mice
8
Y-maze Spontaneous Alternation Assay
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Mice were placed at the end of one arm of the Y-maze apparatus (San Diego Instruments) and allowed to move freely for 5 min. The distance traveled and series of arm entries were recorded using video tracking software EthoVision XT 15 (Noldus). An alternation was defined as entries into all three arms on consecutive occasions. The number of maximum alternations was therefore the total number of arm entries minus two, and the percentage of alternations was calculated.
9
Y-Maze Spontaneous Alternation Assay
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Mice were placed at the end of one arm of the Y-maze apparatus (San Diego Instruments) and allowed to move freely for 5 min. The distance traveled and series of arm entries were recorded using video tracking software EthoVision XT 15 (Noldus). An alternation was defined as entries into all three arms on consecutive occasions. The number of maximum alternations was therefore the total number of arm entries minus two, and the percentage of alternations was calculated.
10
Y-Maze Apparatus for Behavioral Testing
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