Rhodamine b isothiocyanate

Manufactured by Merck Group

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Rhodamine B isothiocyanate is a fluorescent dye commonly used as a labeling agent in various biological applications. It has an excitation maximum of approximately 543 nm and an emission maximum of around 572 nm, making it suitable for detection and visualization techniques.

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Lab products found in correlation

Fluorescein isothiocyanate (fitc), by Merck Group (11 mentions) Dimethyl sulfoxide (dmso), by Merck Group (11 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (10 mentions) Bovine serum albumin (bsa), by Merck Group (9 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (8 mentions) Tetraethyl orthosilicate, by Merck Group (6 mentions) N hydroxysuccinimide, by Merck Group (6 mentions) 3 aminopropyltriethoxysilane, by Merck Group (4 mentions) Silver nitrate, by Merck Group (4 mentions) Ethanol, by Merck Group (4 mentions) Methanol, by Merck Group (4 mentions) Sodium hydroxide (naoh), by Merck Group (4 mentions) Trypsin, by Thermo Fisher Scientific (4 mentions) N n dimethylformamide (dmf), by Merck Group (4 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (4 mentions) α pinene, by Merck Group (3 mentions) L carvone, by Merck Group (3 mentions) 2 phenylethanol, by Merck Group (3 mentions) Acrylamide, by Merck Group (3 mentions) Ammonium hydroxide, by Thermo Fisher Scientific (3 mentions)

96 protocols using rhodamine b isothiocyanate

Synthesis and Characterization of PEG-g-PVAc Copolymer

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Poly(ethylene glycol)
(PEG; molecular weight
(MW), 6 kDa), poly(vinyl acetate) (PVAc; MW, 7 kDa), and PEG-g-PVAc were products of BASF. PEG-g-PVAc
is characterized by a PEG/VAc weight ratio of 40/60; M_n = 13.1 kDa, M_w = 27.5 kDa
(polydispersity index (PDI) = 2.1), and a degree of branching of 1–2%.^{21 (link)} For confocal microscopy imaging purposes, PEG-g-PVAc was covalently labeled with rhodamine B isothiocyanate,
according to a previously described procedure.^{22 (link)}The following reagents were purchased from Sigma-Aldrich
(Milan, Italy) and used as received: L-carvone (Car, ≥97%,
(FCC, FG), log K_ow = 2.74; MW,
150.22 g mol^–1); 2-phenyl ethanol (PE, ≥99.0%
(GC), log K_ow = 1.36; MW, 122.16
g mol^–1); α-pinene (Pin, ≥99.0%, log K_ow = 4.44; MW, 136.23 g mol^–1); rhodamine B isothiocyanate (mixed isomers; MW, 536.08 g mol^–1); D₂O (deuterium content >99%). Water
used in this work was of Milli-Q grade (18.2 MΩ cm at 25 °C).

Mamusa M., Sofroniou C., Resta C., Murgia S., Fratini E., Smets J, & Baglioni P. (2020). Tuning the Encapsulation of Simple Fragrances with an Amphiphilic Graft Copolymer. ACS Applied Materials & Interfaces, 12(25), 28808-28818.

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Polymer-Based Fluorescent Nanoparticle Synthesis

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All the solvents (dimethylformamide, acetone) and P(S-co-MMA) (Aldrich 462896, average Mw 100,000–150,000, pellets, styrene 40%) were purchased from Aldrich; all the solvents were technical grade. The dialysis cellulose membrane (width 10 mm, Sigma Aldrich D9277-100FT) was from Aldrich. Rhodamine B isothiocyanate (RITC, 90% pure) and fluorescein isothiocyanate (FITC, 90% pure) were purchased from Aldrich.

Bertelà F., Battocchio C., Iucci G., Ceschin S., Di Lernia D., Mariani F., Di Giulio A., Muzzi M, & Venditti I. (2023). Dye-Doped Polymeric Microplastics: Light Tools for Bioimaging in Test Organisms. Polymers, 15(15), 3245.

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Synthesis of Magnetic RAFT Nanoparticles

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Reversible addition-fragmentation chain-transfer (RAFT) agents 2-[(butylsulfanyl)carbonothioyl]sulfanyl propanoic acid (C 4 -RAFT) and methoxy-polyethylene glycol modi ed 2-[(butylsulfanyl)carbonothioyl] sulfanyl propanoic acid were kindly provided by Dr Algi Serelis (DuluxGroup). Fe(II) chloride tetrahydrate (99%), Fe(III) chloride hexahydrate (98%), Fe(III) nitrate nonahydrate (99%), acrylamide (>98%), rhodamine B isothiocyanate (>70%), DOX (>98%) and nitric acid (HNO 3 , 65%, Suprapur®, Millipore) were purchased from Sigma-Aldrich (St. Louis, USA). Ammonium hydroxide (28% NH 3 in water) and sodium hydroxide (NaOH) (>98%) were obtained from Ajax Finechem (Australia). 1,4-Dioxane (Fluka-Sigma-Aldrich, St Louis, USA), monoacryloxyethyl phosphate (MAEP, >98%, PolySciences, Warrington, USA) and 4,4azobis(4-cyanovaleric acid) (Wako, USA) were used as received.

Raviraj V., Pham B.T.T., Kim B.J., Pham N.T., Kok L.F., Painter N., Delic N.C., Jones S.K., Hawkett B.S., & Lyons J.G. (2021). Non-Invasive Transdermal Delivery of Chemotherapeutic Molecules In Vivo Using Superparamagnetic Iron Oxide Nanoparticles.

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RAFT-Mediated Polymer Synthesis and Drug Delivery

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Reversible addition-fragmentation chain-transfer (RAFT) agents 2-[(butylsulfanyl)carbonothioyl]sulfanyl propanoic acid (C 4 -RAFT) and methoxy-polyethylene glycol modified 2-[(butylsulfanyl)carbonothioyl]sulfanyl propanoic acid were kindly provided by Dr Algi Serelis (DuluxGroup). Fe(II) chloride tetrahydrate (99%), Fe(III) chloride hexahydrate (98%), Fe(III) nitrate nonahydrate (99%), acrylamide (> 98%), rhodamine B isothiocyanate (> 70%), DOX (> 98%) and nitric acid (HNO 3 , 65%, Suprapur ® , Millipore) were purchased from Sigma-Aldrich (St. Louis, USA). Ammonium hydroxide (28% NH 3 in water) and sodium hydroxide (NaOH) (> 98%) were obtained from Ajax Finechem (Australia). 1,4-Dioxane (Fluka-Sigma-Aldrich, St Louis, USA), monoacryloxyethyl phosphate (MAEP, > 98%, PolySciences, Warrington, USA) and 4,4-azobis(4-cyanovaleric acid) (Wako, USA) were used as received.

Raviraj V., Pham B.T.T., Kim B.J., Pham N.T., Kok L.F., Painter N., Delic N.C., Jones S., Hawkett B.S., & Lyons J.G. (2021). Non-invasive transdermal delivery of chemotherapeutic molecules in vivo using superparamagnetic iron oxide nanoparticles. Cancer Nanotechnology, 12(1).

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Lysozyme Encapsulation and Characterization

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Lysozyme (chicken egg-white), Micrococcus lysodeikticus, isosorbide dimethyl ether (1,4:3,6-dianhydro-2,5-di-O-methyl-Dglucitol), Rhodamine B isothiocyanate (RITC) and Lutrol® F68 were obtained from Sigma-Aldrich (Saint Quentin Fallavier, France). Uncapped 75/25 PLGA provided by Phusis (Saint-Ismier, France) had a mean molecular weight of 21 000 Da (polydispersity index I ¼ 1.8) as determined by size-exclusion chromatography (standard: polystyrene). CO 2 (purity of 99.995%) was obtained from Solfrance (Saint Ouenl'Aumone, France). Ethanol and acetonitrile (HPLC quality) were purchased from Fischer Scientic and triuoroacetic acid (TFA, HPLC quality 37% w/w) was obtained from Carlo Erba, France. A lter of 0.2 mm for HPLC analysis was purchased from Acrodisc, PALL, USA. Phosphate buffered saline (PBS) was purchased from Lonza biowhittaker®, Belgium. Puried water was obtained from a Milli-Q® Advantage A10 system (Millipore, Paris, France). The micro-BCA protein assay reagent kit was purchased from Pierce (Bezons, France). All samples were lyophilized in a freeze-dryer (Lyovax GT, Steris®, France) for 18 hours.

Tran MK ., Swed A ., Calvignac B ., Dang KN ., Hassani LN ., Cordonnier T , & Boury F . (2015). Preparation of polymeric particles in CO₂ medium using non-toxic solvents: discussions on the mechanism of particle formation. Journal of materials chemistry. B, 3(8).

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Glioblastoma Cell Culture Protocols

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Solutions, media and reagents. 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT), Hoechst 33342, Rhodamine B-isothiocyanate, PEG-PLGA, propidium iodide (PI), acetazolamide (A6011-10G), temozolomide (T2577), and goat serum (G-9023) were purchased from Sigma-Aldrich (Oakville, ON, Canada). U251N human glioblastoma cell line was provided by Dr. Josephine Nalbantoglu (Montreal Neurological Institute, Montreal, QC, Canada). U251N cell line was originally obtained from the American Type Culture Collection. Human IgG1 anti-CA IX (1.8 mg/mL, Ab $ 150 kDa) was acquired from Dr. Nathan Yoganathan (Kalgene Pharmaceuticals, Kingston, Ontario). Mouse anti-human CA IX-MAb was purchased from Abcam Ò (Cambridge, MA, USA Cat# ab107257). Cell culture media, penicillin-streptomycin, and heatinactivated fetal bovine serum (FBS) were purchased from Invitrogen (Burlington, ON, Canada).

Amiri A., Le P.U., Moquin A., Machkalyan G., Petrecca K., Gillard J.W., Yoganathan N, & Maysinger D. (2016). Inhibition of carbonic anhydrase IX in glioblastoma multiforme. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 109.

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Fabrication of PDMS-Based Functional Surfaces

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Unless stated otherwise, all
chemicals were used as purchased. PDMS preparation is performed by
using the commercially available standard kit of SYLGARD 184 from
Dow Corning. N-[Tris(hydroxymethyl)methyl]acrylamide
(contains ≤7% KCl, 93%), 4,4′-azobis(4-cyanovaleric
acid) (>98%), (3-aminopropyl)triethoxysilane (APTES, >99.8%),
chorotrimethylsilane
(98%), ammonium hydroxide solution (28.0–30.0% w/w NH₃ basis), trimethylamine (99.5%), rhodamine B isothiocyanate (mixed
isomers), and rhodamine 6G (95%) were purchased from Sigma-Aldrich.
NaOH (>99.8%), ethanol (96%), diethyl ether (≥99.5%), and
acetic
acid (99.5%) were obtained from Chemsolute. 1,4-Dioxane (>99.5%)
and
dichloromethane (>99.8%) were purchased from Roth. N,N-Dimethylformamide (99.8%) was obtained from Merck.
Hydrogen peroxide (30%), N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimid hydrochloride (99%), and calcium
chloride (>98%) were used as it is purchased from Roth. Plasma
treatment
for the surface activation was performed using a PlasmaFlecto 10 oven.

Akarsu P., Grobe R., Nowaczyk J., Hartlieb M., Reinicke S., Böker A., Sperling M, & Reifarth M. (2021). Solid-Phase Microcontact Printing for Precise Patterning of Rough Surfaces: Using Polymer-Tethered Elastomeric Stamps for the Transfer of Reactive Silanes. ACS Applied Polymer Materials, 3(5), 2420-2431.

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Fluorescent Protein Labeling Protocol

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2 mg/ml of pentapeptide HPHGH (PPT), commercially available lysozyme (LYS-Sigma), and bovine serum albumin (BSA-New England-Biolabs) were labeled with 50 µl of Rhodamine B isothiocyanate (Sigma) (1 mg/ml in DMSO) as specified by the manufacturer. The protocol is based on the reaction between the isothiocyanate group of Rhodamine and epsilon-NH₂ of Lysine residues of the protein to be labeled in order to obtain a fluorescent complex. Final molar Rhodamine/Proteins ratio was 0.06 and the labeling reactions were performed pH 8.5. The labeling was followed by dialysis in 1×PBS to remove the unbound fluorescent excess and lyophilization.

Petrillo C., Castaldi S., Lanzilli M., Saggese A., Donadio G., Baccigalupi L., Ricca E, & Isticato R. (2020). The temperature of growth and sporulation modulates the efficiency of spore-display in Bacillus subtilis. Microbial Cell Factories, 19, 185.

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Biomaterial Synthesis and Characterization

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Cystamine dihydrochloride (CYS), 1.1 Carbonyldiimidazole (CDI), sodium alginate (Alginate, MW determined by viscosimetry was 140 kDa), dextran sulfate sodium salt (Dextran, MW 40 kDa), carboxymethyl-dextran sodium salt (CM-Dextran-MW 70 kDa), hyaluronic acid (low MW HA, 20–70 kDa), Rhodamine B isothiocyanate (RITC), DL-Dithiothreitol 99% (DTT), bovine serum albumin, 96% (BSA), dimethyl sulfoxide (DMSO), and lysozyme from chicken egg white, 90% were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Caso M.F., Carotenuto F., Di Nardo P., Migliore A., Aguilera A., Lopez C.M., Venanzi M., Cavalieri F, & Rinaldi A. (2020). Nanoporous Microsponge Particles (NMP) of Polysaccharides as Universal Carriers for Biomolecules Delivery. Nanomaterials, 10(6), 1075.

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Synthesis of Fluorescent Silica Nanoparticles

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All chemicals were used as received, unless indicated otherwise. Tetraethyl orthosilicate (TEOS), (3-aminopropyl)triethoxysilane (99%) and rhodamine B isothiocyanate (C 29 H 30 ClN 3 O 3 S, l ex 543 nm, l em 580 nm in methanol) were purchased from Sigma Aldrich. n-Hexylamine and ethanol anhydrous (99.8%) purchased from Merck-Chemicals. Toluene (AR) was purchased from Biosolve. Fluorescein 5(6)-isothiocyanate (C 21 H 11 NO 5 S, l ex = 495 nm, l em = 525 nm) (90% HPLC) was purchased from Fluka. The water used was double de-ionized water produced by an Elix Millipore purification system.

van Wijk J ., Salari JWO ., Meuldijk J , & Klumperman B . (2015). Determination of the shell growth direction during the formation of silica microcapsules by confocal fluorescence microscopy. Journal of materials chemistry. B, 3(39).

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