Mgfap cre line 77

The following wildtype or transgenic mouse lines were obtained from the Jackson Laboratory: C57BL/6J (stock #000664), Aldh1l1-CreER^T2 (stock #029655) (Srinivasan et al., 2016 (link)), mGfap-Cre line 77.6 (stock #024098) (Gregorian et al., 2009 (link)), R26R-YFP (stock #006148) (Srinivas et al., 2001), and R26R-tdTomato (stock #007914) (Madisen et al., 2010). Hemizygous Aldh1l1-CreER^T2 males or females were used for breeding, whereas only hemizygous females of mGfap-Cre were used for breeding. After breeding to the reporter mouse lines, cell type-specificity of the traced cells was extensively examined by immunohistochemistry in different brain regions. Of note, except for the core striatum the mGfap-Cre line shows variable leaky expression in neurons of different brain regions including the cortex and midbrain. Both adult male and female mice at 8 weeks or older were used for most of the experiments, whereas young mice at postnatal day (P) 35 to 40 days were used for the shRNA experiments as described (Qian et al., 2020 (link)). All mice were housed under controlled temperature and a 12-h light/dark cycle with free access to water and food in an animal barrier facility at UT Southwestern. Animal procedures were approved by the Institutional Animal Care and Use Committee at UT Southwestern.