Rabbit monoclonal anti ki67

Manufactured by Abcam

Rabbit monoclonal anti-Ki67 is a primary antibody that detects the Ki-67 protein, a nuclear marker of cellular proliferation. It is commonly used in immunohistochemistry and flow cytometry applications to identify and quantify proliferating cells.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Agilent Technologies (1 mentions) Fv1000 confocal microscope, by Olympus (1 mentions) Lsm710 system, by Zeiss (1 mentions) 6 diamidino 2 phenylindole dapi, by Thermo Fisher Scientific (1 mentions) Rabbit monoclonal anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Fv10 asw 2, by Olympus (1 mentions) Fluoview fv1000 confocal microscope, by Olympus (1 mentions) Vectashield antifade solution, by Vector Laboratories (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Anti-Ki67 (637 citations) Rabbit anti-Ki67 (202 citations) Rabbit anti- (5 citations) Anti-Ki67 rabbit monoclonal antibody (5 citations) Rabbit anti-mouse Ki67 monoclonal antibody (2 citations)

3 protocols using rabbit monoclonal anti ki67

Immunofluorescent Staining of Paraffin-Embedded Tissues

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For deparaffinized tissue sections (5 μm thick), antigen retrieval was performed in Tris-EDTA Buffer (10 mM Tris Base, 1 mM EDTA Solution, 0.05% Tween 20, pH 9.0). Subsequently, the tissue sections were blocked with goat serum for 1h and incubated with primary antibodies (mouse monoclonal anti-MelanA, Abcam; rabbit monoclonal anti-Ki67, Abcam; 1:200) overnight at 4°C. After washed with PBS, they were incubated with secondary antibodies (FITC or Cy3-tagged goat anti-rabbit, 1:200) for 1h, and then washed with PBS and further incubated with DAPI (1:1000, Dako, Glostrup, Denmark) for 15 min. Fluorescent images were obtained by an FV-1000 confocal microscope (Olympus, Tokyo, Japan).

Ma J., Shi Q., Guo S., Xu P., Yi X., Yang Y., Zhang W., Liu Y., Liu L., Yue Q., Zhao T., Gao T., Guo W, & Li C. (2022). Long Non-Coding RNA CD27-AS1-208 Facilitates Melanoma Progression by Activating STAT3 Pathway. Frontiers in Oncology, 11, 818178.

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Immunofluorescence Staining for Cell Marker Analysis

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For immunofluorescence staining, sections were blocked with PBS 2% goat serum 0.3% Triton X-100 for one hour at RT, and then incubated with rabbit monoclonal anti-Ki-67 (Abcam, 1:200), rabbit monoclonal anti-cleaved caspase 3 (Cell Signaling Technology, 1:200), or mouse monoclonal anti-EBP50 (BD Biosciences, 1:50) for one hour at 37°C. After washing in PBS, slides were incubated for one hour at RT with goat anti-mouse or anti-rabbit Alexa Fluor 488- or 546-conjugated antibodies (Invitrogen, 1:800). During the last ten minutes of incubation 4', 6-diamidino-2-phenylindole (DAPI, Invitrogen, 1:100) was added. Sections were analyzed by confocal laser-scanning microscope (Zeiss LSM-710 system, Carl Zeiss Microimaging GmbH) or by fluorescence microscope (Olympus BX61, Olympus Inc.).

Däster S., Amatruda N., Calabrese D., Ivanek R., Turrini E., Droeser R.A., Zajac P., Fimognari C., Spagnoli G.C., Iezzi G., Mele V, & Muraro M.G. (2016). Induction of hypoxia and necrosis in multicellular tumor spheroids is associated with resistance to chemotherapy treatment. Oncotarget, 8(1), 1725-1736.

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Immunofluorescence Analysis of Primary Mouse Endothelial Cells

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Primary mouse ECs grown on collagen-coated coverslips in 24-well culture dishes were fixed in 4% paraformaldehyde and then blocked with donkey serum in 0.3 M glycine in PBS for 1 h at room temperature. The cells were then incubated with rabbit monoclonal anti-Ki67 (1:200, Abcam) or rabbit polyclonal anti-VE-cadherin primary antibodies (1:200, Abcam) overnight at 4°C, followed by incubation with donkey anti-rabbit secondary antibody conjugated with Cy3 (1:1000, Jackson ImmunoResearch Laboratories, Inc.). The cells were then counterstained with DAPI for 2 min at room temperature. After washing in PBS, the coverslips were mounted on glass slides with antifade Vectashield solution (Vector Laboratories). Fluorescence images were captured with an Olympus Fluoview FV1000 confocal microscope with FV10-ASW 2.0 software (Olympus America).

Wang J., Shi Y., Zhang L., Zhang F., Hu X., Zhang W., Leak R.K., Gao Y., Chen L, & Chen J. (2014). Omega-3 polyunsaturated fatty acids enhance cerebral angiogenesis and provide long-term protection after stroke. Neurobiology of disease, 68, 91-103.

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