Marine organism-derived EVs were visualized using TEM (H-7500; Hitachi, Tokyo, Japan) and SEM (MIRA 3 LMH; TESCAN, Brno, Czech Republic). Electron microscopy analysis was performed according to a previously described protocol. The mEV suspension (100 μg) was fixed with 4% paraformaldehyde for 30 min and washed with PBS. Subsequently, fixed EVs were dehydrated with an ascending sequence of ethanol (80%, 90%, 95%, and 100%). The mEV suspension was dropped on a cover glass or carbon-coated grid (TED PELLA, Redding, CA, USA) and left in fume hoods for 24 h.
Mira 3 lmh
Manufactured by TESCAN
Sourced in Czechia, Japan
The MIRA 3 LMH is a high-performance scanning electron microscope (SEM) designed for a wide range of applications. It features a compact and ergonomic design, advanced electron optics, and user-friendly software to provide high-quality imaging and analysis capabilities.
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Lab products found in correlation
Nicolet is50, by Thermo Fisher Scientific (3 mentions)
H 7500, by Hitachi (2 mentions)
Jem 2100f, by JEOL (2 mentions)
Autolab pgstat302n, by Metrohm (2 mentions)
Carbon coated grid, by Ted Pella (1 mentions)
Cpa 52z, by Ametek (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
Lext 4100, by Olympus (1 mentions)
Oca 15 plus, by Dataphysics (1 mentions)
Ag x plus, by Shimadzu (1 mentions)
D8 advance, by Bruker (1 mentions)
Empyrean series 2 x ray diffractometer, by Malvern Panalytical (1 mentions)
Spectrum 65 spectrometer, by PerkinElmer (1 mentions)
Ultima 4, by Rigaku (1 mentions)
Tga dsc1, by Mettler Toledo (1 mentions)
Quantax 200, by Bruker (1 mentions)
Thermo scientific k alpha, by Thermo Fisher Scientific (1 mentions)
Agn 250, by Shimadzu (1 mentions)
X pert powder, by Malvern Panalytical (1 mentions)
D max 2500, by Rigaku (1 mentions)
31 protocols using mira 3 lmh
1
Visualizing Marine Organism-Derived EVs
2
Characterizing Rubbery Composite Thermal and Electrical Properties
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After the degassed mixed paste was poured onto a fluorine release film, the viscosity of the residual degassed mixed paste without curing was measured using a viscometer (Brookfield, DV Next HB series, Middleborough, MA, USA) with a cone-and-plate-type spindle (CPA-52Z) at 1 rpm and 25 °C. Thermal conductivity was measured at room temperature using a TCi thermal conductivity analyzer (C-THERM, Fredericton, NB, Canada). The rubbery composite pads needed to be at least 40 mm × 40 mm × 3 mm for the thermal conductivity measurements. A 500 g weight was placed on the sample, which was then placed on the sensor, to ensure conformal contact between the sensor and the sample. Thermal conductivity measurements were repeated 9 times for each sample. The instrument for thermal conductivity measurements was displayed to the third decimal place. After curing, the rubbery composite pads were sliced using a razor blade and coated with a thin layer of Pt. These rubbery composite pads were imaged using a high-resolution scanning electron microscope (HR FE-SEM, Tescan, MIRA3-LMH, Brno, Czech Republic) equipped with an energy dispersive X-ray analyzer (EDS). Elemental mappings of Si, Al, Zn, and Cu were performed using the EDS. The electrical insulating performance of the rubbery composite pads was tested using a surface resistance checker (Surpa, model 385, Shenzhen, China).
3
Characterization of Micellar Nanoparticles
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The size distributions of the DAN/DAB-PPS-mPEG micelles and Nile Red loaded micelles were measured using a Malvern Instruments Nano ZS90 equipped with a 633 nm He-Ne gas laser. The micelles were imaged using a Tescan MIRA 3 LMH scanning electron microscope (SEM). The micellar dispersion was suspended in water, deposited on silicon wafers and left to dry overnight and imaged. Additionally, the fluorescence intensities of the micelles dispersions prepared with DAN-PPS-mPEG mixed with different amount of NH2-PPS-mPEG (1 mg/mL) were recorded to test the possible self-quenching effect of the dansyl fluorophore.
4
Wettability Analysis of Surface Samples
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The morphology of all the samples was examined by the field-emission scanning electron microscopy (SEM, TESCAN MIRA 3 LMH) equipped with an energy-dispersive spectroscope (EDS, Oxford). The 3D topography measurement was characterized using a 3D digital optical microscope (OLYMPUS LEXT 4100).
Contact angle and sliding angle measurements were carried out to evaluate the wettability of various surfaces by a video-based optical contact angle measuring device (OCA 15 Plus from Data Physics Instruments). The droplets in this work are deionized and purified water with a volume of 5 μL. The contact and sliding angles of every sample were examined at different randomly selected locations at least three times.
Contact angle and sliding angle measurements were carried out to evaluate the wettability of various surfaces by a video-based optical contact angle measuring device (OCA 15 Plus from Data Physics Instruments). The droplets in this work are deionized and purified water with a volume of 5 μL. The contact and sliding angles of every sample were examined at different randomly selected locations at least three times.
5
Characterization of Material Tensile Properties
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The morphology and composition of the aforementioned materials were characterized by scanning electron microscopy (SEM, Tescan MIRA3 LMH, Brno, Czech Republic) and X-ray diffractometer (XRD, Bruker D8 Advance, Karlsruhe, Germany). The tensile properties were measured on a universal material testing machine (AG-X plus, Shimadzu Corporation, Shimane, Japan) at a deformation rate of 50 mm min−1 at room temperature.
6
Granule Particle Size and Tablet Properties
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We investigated the effect of the tablet properties on the granule particle size of the SR layer [17 (link)]. A stereo binocular microscope (SMZ18 Contact Scope, Nikon, Tokyo, Japan) and scanning electron microscope (SEM, MIRA3LMH, Tescan, Brno-Kohoutovice, Czech Republic) were used to confirm the differences in particle size of the granules used for each formulation. The flowability of the mixture of each formulation prepared using the three manufacturing methods was calculated using Carr’s index (CI) [18 (link)]. The bulk and tap densities were measured using a graduated measuring cylinder. The bulk density is defined as the mass of many particles in the powder sample divided by their total occupied volume. The tap density was obtained after mechanically tapping a graduated measuring cylinder containing the powder sample. The flowability formula is shown below. A CI value of ≥25 indicates poor flowability, whereas a CI value of ≤15 indicates good flowability.
An in-process control was performed for each prepared tablet. Hardness was measured using a Screw Test Stand (ALX-J, Wenzhou Tripod Instrument Manufacturing Co., Ltd., Wenzhou, China) in units of kilopond (kp). The tableting pressure was confirmed by measuring the tableting pressure of the tableting machine in units of kilonewtons.
An in-process control was performed for each prepared tablet. Hardness was measured using a Screw Test Stand (ALX-J, Wenzhou Tripod Instrument Manufacturing Co., Ltd., Wenzhou, China) in units of kilopond (kp). The tableting pressure was confirmed by measuring the tableting pressure of the tableting machine in units of kilonewtons.
7
Comprehensive Characterization of Silver Nanoparticles
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The morphology and elemental composition of the samples were studied using a MIRA 3 LMH (Tescan, Brno, Czech Republic) scanning electron microscope (SEM) with an Aztec Energy Standard/X-max 20 (standard) system for determining the elemental composition by energy-dispersive X-ray spectroscopy (EDX).The average hydrodynamic radius of silver nanoparticles was measured by photon-correlation spectroscopy (PCS) using a Photocor complex device (Photocor, Moscow, Russia).The phase composition of the samples was investigated by X-ray diffraction analysis on an Empyrean series 2 X-ray diffractometer (PANalytical, Almedo, The Netherlands). Particle size in the samples was measured by the electroacoustic spectroscopy method using a DT-1202 analyzer (Dispersion Technology Inc., New York, NY, USA) [17 (link)]. The particle size distribution of the CeO2-Ag samples was measured by LDA on a Shimadzu SALD-7500 nano-laser particle size analyzer (Shimadzu Corp., Kyoto, Japan).
8
Comprehensive Material Characterization Techniques
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X-Ray diffraction (XRD) was performed on a Rigaku Ultima IV equipped with a copper target. Fourier-transform infrared spectroscopy (FTIR) spectra were recorded on a PerkinElmer Spectrum 65 spectrometer between 4000 and 450 cm−1 with a resolution of 8 cm−1, averaging 5 scans per sample. Raman spectroscopy was carried out at room temperature on a custom-built setup using an excitation wavelength of 633 nm at a power of 60 mW (LuxX633, Omicron), and acquisition times of 0.5 s. Scanning electron microscope (SEM) images were acquired on a Tescan Mira 3 LMH scanning electron microscope at accelerating voltages of 10 to 20 kV. Thermogravimetric analysis (TGA) was performed on a Mettler Toledo TGA/DSC 1 instrument in a temperature range of 25 to 600 °C with a heating rate of 10 °C min−1 under N2 flow of 30 mL min−1. The specific surface area and the pore size distribution of the samples were determined with a Micromeritics Gemini V surface area and pore size analyzer.
9
Carbon Coating for Electron Microscopy
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An 8–10 nm-thick carbon layer, for prevention of charging during electron microanalysis, covered all the samples (carbon coater Leica EM ACE600, Leica Microsysteme, Germany). A Schottky-cathode (3 kV of accelerating voltage) scanning electron microscope MIRA 3 LMH (Tescan company, Czech Republic) was used for morphology investigation of the prepared samples.
10
SEM and EDX Analysis of Sample
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MIRA 3 LMH (Tescan, Czech Republic) using 10 kV of accelerating voltage was employed for SEM measurement. Magnification was 30 000×. Energy dispersive X-ray spectroscopy (EDX) EDX measurement were performed by Bruker Quantax 200 with 6|10 XFlash detector using 15 kV of accelerating voltage.
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