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3 protocols using anhydrous sodium acetate naac

1

Quantitative Proteomic Sample Preparation

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N-Ethylmaleimide, 6-aminocaproic acid, benzamidine hydrochloride hydrate, dithiothreitol (DTT), iodoacetamide, ammonium bicarbonate (AMBIC), formic acid, and HPLC grade acetonitrile and Solvents A (0.1% formic acid) and B (0.1% formic acid in acetonitrile) for liquid chromatography-mass spectrometry (LC-MS) were from Sigma–Aldrich (St. Louis MO, USA). Guanidine hydrochloride (GdnHCl) and anhydrous sodium acetate (NaAc) were from Merck (Darmstadt, Germany). Trypsin Gold (MS grade) was purchased from Promega (Madison WI, USA). The Pierce Quantitative Colorimetric Peptide Assay and SOLAμ™ Solid Phase Extraction (SPE) HRP (horse radish peroxidase) 2mg/1 ml 96-well plates were from Thermo Fisher Scientific (Rockford IL, USA), and Nanosep® 30K Omega Centrifugal Devices were from Pall Life Sciences (Ann Arbor MI, USA).
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2

Protein Identification and Quantification

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N-Ethylmaleimide, 6-aminocaproic acid, benzamidine hydrochloride hydrate, dithiothreitol (DTT), iodoacetamide, ammonium bicarbonate (AMBIC), formic acid, HPLC grade acetonitrile and A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile) solutions for LC-MS were purchased from Sigma-Aldrich (St. Louis, USA). Guanidine hydrochloride (GdnHCl) and anhydrous sodium acetate (NaAc) were purchased from Merck (Darmstadt, Germany). Trypsin gold MS grade was purchased from Promega (Madison, WI). The water used in this study was purified using a MilliQ apparatus (Millipore, Billerica, MA).
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3

Quantitative Proteomic Sample Preparation

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N-Ethylmaleimide, 6-aminocaproic acid, benzamidine hydrochloride hydrate, dithiothreitol (DTT), iodoacetamide, ammonium bicarbonate (AMBIC), formic acid, and high-performance liquid chromatography (HPLC) grade acetonitrile and Solvents A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile) for LC-MS were purchased from Sigma-Aldrich (St. Louis). Guanidine hydrochloride (GdnHCl) and anhydrous sodium acetate (NaAc) were purchased from Merck (Darmstadt, Germany). Trypsin Gold (MS grade) was purchased from Promega (Madison, WI). The water used in this study was purified using a MilliQ apparatus (Millipore, Billerica, MA). The Pierce Quantitative Colorimetric Peptide Assay was purchased from Thermo Fisher Scientific (Rockford) and Nanosep 30K Omega Centrifugal Devices were purchased from Pall Life Sciences (Ann Arbor). Reversed-phase C18 columns were purchased from Nest Group (Southborough, MA).
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