Ki67 antibody ab1667

Manufactured by Abcam

The Ki67 antibody (ab1667) is a protein that is used to detect the presence and expression of the Ki67 protein, which is a marker of cellular proliferation. The Ki67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). The Ki67 antibody can be used in various applications, such as immunohistochemistry, to assess the proliferative state of cells in tissue samples.

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Ki67 antibody (174 citations) Ab1667 (18 citations)

3 protocols using ki67 antibody ab1667

TNBC Cell Lines Characterization

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Human TNBC cell lines: BT-549, HCC-1806, HCC-1937, MDA-MB-231, MDA-MB-453, MDA-MB-468 and SUM-159 were obtained from the American Type Culture Collection (ATCC) and were maintained using ATCC recommended media. All model cells utilized were free of mycoplasma contamination. Additionally, STR DNA profiling was used to confirm the identity of cell lines. The GAPDH, p-ERK (42/44), ERK (42/44), p-S6 (s235/236), S6, p-mTOR (S2448), mTOR, p-4EBP1, 4EBP1 and c-Myc antibodies were obtained from Cell Signaling Technology (Beverly, MA). LAS1L, TEX-10, SENP3 antibodies were purchased from Proteintech (Rosemont, IL). The β-Actin antibody (A-2066), WDR18 antibody (HPA050200) and Vinculin antibody (V9264) were purchased from Millipore Sigma (Burlington, MA). The Ki67 antibody (ab1667) was purchased from Abcam (Cambridge, MA). The PELP1 antibody (A300-180A) was purchased form Bethyl Laboratories Inc. (Montgomery, TX).

Altwegg K.A., Pratap U.P., Liu Z., Liu J., Sanchez J.R., Yang X., Ebrahimi B., Panneerdoss D.M., Li X., Sareddy G.R., Viswanadhapalli S., Rao M.K, & Vadlamudi R.K. (2023). Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34. Breast Cancer Research and Treatment, 200(1), 151-162.

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Characterization of Breast Cancer Cell Lines

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Human WT- ER⁺ BC cells MCF7, ZR75, and T47D, human mammary epithelial cells (HMEC), murine mammary epithelial cells (HC11), primary human endometrial epithelial cells (HEEC), primary human endometrial stromal cells (HESC), and primary human nontumorigenic immortalized ovarian surface epithelial cells (IOSE-80) were obtained from the American Type Culture Collection (ATCC) and were maintained using ATCC recommended media. MCF7 and ZR75 model cells stably overexpressing PELP1 cDNA or PELP1-shRNA have been described previously (18 (link)). MCF7-TamR (tamoxifen resistant) and MCF7-LTLT (letrozole resistant) cells were cultured in tamoxifen or letrozole (1 μM) containing media (19 ). MCF7^(Y537S) and MCF7^(D538G) MT- ER⁺ BC cell lines were described previously (20 (link)). ZR75^(Y537S) and ZR75^(D538G) MT- ER⁺ BC cell lines were generated in our lab (19 ). All model cells utilized were free of mycoplasma contamination. Additionally, STR DNA profiling was used to confirm the identity of cells. The GAPDH (8884) antibody was obtained from Cell Signaling Technology (Beverly, MA). The β-Actin (A-2066) and Vinculin antibodies (V9264) were purchased from Millipore Sigma (Burlington, MA). The Ki67 antibody (ab1667) was purchased from Abcam (Cambridge, MA). The PELP1 antibody (A300-180A) was purchased form Bethyl Laboratories Inc. (Montgomery, TX).

Altwegg K.A., Viswanadhapalli S., Mann M., Chakravarty D., Krishnan S., Liu Z., Liu J., Pratap U.P., Ebrahimi B., Sanchez J.R., Li X., Ma S., Park B.H., Santhamma B., Chen Y., Lai Z., Raj G.V., Yuan Y., Zhou D., Sareddy G.R., Tekmal R.R., McHardy S., Huang T.H., Rao M.K., Vankayalapati H, & Vadlamudi R.K. (2022). A first-in-class inhibitor of ER coregulator PELP1 targets ER+ breast cancer. Cancer research, 82(20), 3830-3844.

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Investigating Leflunomide and AICAR Effects

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Leflunomide was purchased from Enzo life sciences (ALX-4300–095-G001). AICAR was purchased from Toronto Research Chemicals Inc (North York, Canada). Uridine was purchased from Sigma (St. Louis, MO). Antibodies against LKB1 (3047s), Caspase-3 (9962s), Cleaved caspase-3 (9664s), PARP (9542s), Kras-G12D (14429), p53 (2524s) were purchased from Cell Signaling Technology (Beverly, MA). Ki67-antibody (ab1667) and TTF-1 antibody (ab76013) were purchased from Abcam (Cambridge, UK). DAB substrate kit was purchased from Vector Laboratories (SK-4100, Burlingame, CA). Mouse monoclonal anti-β-actin antibody (a1978) was purchased from Sigma (St. Louis, MO). Matrigel was purchased from Corning (Cat#356255, Corning, NY).

Jin R., Liu B., Liu X., Fan Y., Peng W., Huang C., Marcus A.I., Sica G.L., Gilbert-Ross M., Liu Y, & Zhou W. (2020). Leflunomide Suppresses the Growth of LKB1-inactivated Tumors in the Immune-Competent Host and Attenuates Distant Cancer Metastasis. Molecular cancer therapeutics, 20(2), 274-283.

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