Log In Sign up
The largest database of trusted experimental protocols

Dmem 1g l glucose

Manufactured by Dutscher
Visit Supplier
Sourced in France

DMEM 1g/L glucose is a cell culture medium formulation that provides essential nutrients for the growth and maintenance of various cell types. It contains 1 gram per liter of glucose as the primary energy source. This medium is suitable for a range of applications in cell biology research and cell culture-related experiments.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin streptomycin, by Dutscher (1 mentions) Fetal bovine serum (fbs), by Corning (1 mentions)

2 protocols using dmem 1g l glucose

1

Breast Cancer Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
MDA-MB-436 and MCF-7 cells were obtained from ATCC (HTB-130 and HTB-22). Cells were cultured at 37°C with 5% CO2 atmosphere in DMEM 1g/L glucose (Dominique Dutscher, L0066) supplemented with 5% SVF (Dominique Dutscher, S1810), 1% penicillin/streptomycin (Dominique Dutscher, L0018) and 0.1% Fungizone (PAA, P11-001). MCF-7 control and MCF-7 overexpressing FLAG-GABARAPL1-6His were available in the laboratory and cultured as described above with the exception of 10% SVF.[16 (link)]
Gauthier T., Claude-Taupin A., Delage-Mourroux R., Boyer-Guittaut M, & Hervouet E. (2015). Proximity Ligation In situ Assay is a Powerful Tool to Monitor Specific ATG Protein Interactions following Autophagy Induction. PLoS ONE, 10(6), e0128701.
+ Open protocol
+ Expand
2

Inducing EMT in Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
A549 (lung adenocarcinoma, ATCC CCL-185) and ACHN (kidney adenocarcinoma, ATCC CRL-1611) cancer cells were cultured in DMEM 1g/l glucose (L0066, Dominique Dutscher, Bernolsheim, France) supplemented with 10% FBS (702774C, Corning, Amsterdam, The Netherlands) and 100 µg/mL penicillin/streptomycin (PAA, P11-010) in a 5% CO2 incubator at 37 °C. To inhibit autophagy, cells were treated with 50 mM NH4Cl for 2 h. To induce EMT, cells were co-treated with 5 ng/mL TGF-β and 20 ng/mL TNF-α from 10 to 24 h.
Jacquet M., Hervouet E., Baudu T., Herfs M., Parratte C., Feugeas J.P., Perez V., Reynders C., Ancion M., Vigneron M., Baguet A., Guittaut M., Fraichard A, & Despouy G. (2021). GABARAPL1 Inhibits EMT Signaling through SMAD-Tageted Negative Feedback. Biology, 10(10), 956.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!