Beckman cx4

Manufactured by Beckman Coulter

Sourced in United States, China, Germany

The Beckman CX4 is a chemical analyzer used in clinical laboratories for the automated processing and analysis of various biochemical assays. It is designed to provide accurate and reliable results for a wide range of clinical chemistry tests.

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Close spelling variants of this product

Beckman CX4 Chemistry Analyzer Beckman CX4 chemistry analyser

28 protocols using beckman cx4

Comprehensive Metabolic Profile Analysis

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Serum glucose (GLU), total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and phospholipid (PL) were measured using commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and an automatic biochemical instrument (Biochemical Analytical Instrument, Beckman CX4; Beckman Coulter Inc., Brea, CA, USA), and the content of very low density lipoprotein (VLDL) was calculated based on the levels of TC, HDL and LDL (VLDL = TC−HDL−LDL). Furthermore, Serum insulin, glucagon, and leptin levels were assayed using commercially available ELISA kits (Beijing Chenglin Biotechnology Co., Ltd., Beijing, China) and an automatic biochemical instrument (Biochemical Analytical Instrument, Beckman CX4; Beckman Coulter Inc., USA). All measurements were conducted in triplicate at minimum according to the manufacturer’s instructions.

Lv G., Zeng Q., Ding X., Bai S, & Zhang K. (2021). Effects of age and diet forms on growth-development patterns, serum metabolism indicators, and parameters of body fat deposition in Cherry Valley ducks. Animal Bioscience, 35(2), 247-259.

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Immunoglobulins and Biochemical Profiling

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Immunoglobulins (IgG and IgM), as well as biochemical indicators (total protein, albumin, etc.) were measured using an instrument (Biochemical Analytical Instrument, Beckman CX4, Beckman Coulter Inc., Brea, CA) and commercial kits (Sino-German Beijing Leadman Biotech Ltd., Beijing, China).

Qi M., Tan B., Wang J., Liao S., Li J., Liu Y, & Yin Y. (2019). Post-natal Growth Retardation Associated With Impaired Gut Hormone Profiles, Immune and Antioxidant Function in Pigs. Frontiers in Endocrinology, 10, 660.

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Serum Biochemical and Hormone Analysis

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Serum biochemical parameters, including total protein (TP), albumin (ALB), alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), glucose (GLU), and lactic dehydrogenase (LDH), were measured using Biochemical Analytical Instrument (Beckman CX4) and commercial kits (Sino-German Beijing Leadman Biotech Ltd, Beijing, China).
Serum samples were treated with sulfosalicylic acid, centrifuged, and filtered through a 0.45 μm filtration membrane. Then the amino acid concentrations were determined using an automatic amino acid analyzer (Model L-8900, Hitachi Ltd. Tokyo, Japan).
The serum concentrations of cholecystokinin (CCK), peptide YY (PYY), ghrelin, glucagon, and leptin were determined using the corresponding pig ELISA Kit (CUSABIO, Wuhan, China) in accordance with the manufacturer's instructions.

Duanmu Q., Tan B., Wang J., Huang B., Li J., Kang M., Huang K., Deng Q, & Yin Y. (2022). The Amino Acids Sensing and Utilization in Response to Dietary Aromatic Amino Acid Supplementation in LPS-Induced Inflammation Piglet Model. Frontiers in Nutrition, 8, 819835.

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Serum Biochemical Indicators in Pigs

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After slaughter, blood samples were collected from the pigs. Serum was separated and used to determine the concentration of serum biochemical indicators, as mentioned before (24 (link)). The concentrations of total protein (TP), albumin (ALB), alkaline phosphatase (ALP), blood urea nitrogen (BUN), glucose (GLU), triglycerides (TG), cholesterol (CHOL), low-density lipoprotein (LDL)-cholesterol, high-density lipoprotein (HDL)-cholesterol, and blood ammonia (NH₃) were analyzed. These indicators were all tested by commercial kits (Leadman Biotech Limited, Beijing, China) and biochemical analytical instruments (Beckman CX4; Beckman Coulter, Germany).

Zhang L., Guo Q., Duan Y., Lin X., Ni H., Zhou C, & Li F. (2022). Comparison of the Effects of Inorganic or Amino Acid-Chelated Zinc on Mouse Myoblast Growth in vitro and Growth Performance and Carcass Traits in Growing-Finishing Pigs. Frontiers in Nutrition, 9, 857393.

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Serum Cytokine and IgG Profiling

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Serum concentrations of interleukin-1β (IL-1β), IL-4, IL-6, IL-8, tumor necrosis alpha (TNF-α) and interferon-gamma (IFN-γ) were measured using commercial pig-specific ELISA kits (Shanghai Kexin Biotech Co., Ltd, Shanghai, China), following the kit instruction. And the serum IgG level was measured using biochemical automatic analyzer (Biochemical Analytical Instrument, Beckman CX4, Beckman Coulter Inc., Brea, CA).

Cheng B., Huang M., Zhou T., Deng Q., Teketay W.a.s.s.i.e., Wu T, & Wu X. (2023). Garlic essential oil supplementation modulates colonic microbiota compositions and regulates immune response in weaned piglets. Heliyon, 9(8), e18729.

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Goat Blood Biomarker Analysis

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After overnight fasting, blood was collected via the jugular vein at 6 am on weeks 0, 4, and 8. Blood samples obtained from each goat were placed into a vacuette tube with lithium heparin (Greiner Bio-One GmbH, Kremsmünster, Austria) and then centrifuged at 3500× g for 5 min at 4 °C to collect serum, which was stored at −80 °C for further analysis. The levels of blood urea nitrogen (BUN), total cholesterol (TC), total triglyceride (TAG), and total protein (TP) were determined using a Biochemical Analytical Instrument (Beckman CX4). The following growth hormone (GH) and SIF commercial ELISA kits were used for hormone determination according to the manufacturer’s instructions: growth hormone (MB-4795A, Jiangsu Meibiao Biotechnology Co., Ltd., Nanjing, China) and SIFs (FT-P9S1826X, Jiangsu Meibiao Biotechnology Co., Ltd., Nanjing, China). All analyses were conducted on the same day and included a standard curve.

Shao Y., Xu J., Wang M., Ren Y., Wei M., Tian B., Luo J., Loor J.J, & Shi H. (2024). Preliminary Results on the Effects of Soybean Isoflavones on Growth Performance and Ruminal Microbiota in Fattening Goats. Animals : an Open Access Journal from MDPI, 14(8), 1188.

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Biochemical Indicator Measurement Protocol

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Biochemical indicators (D-LACT: mmol/L, DAO: mmol/L) were measured using an instrument (Biochemical Analytical Instrument, Beckman CX4, Beckman Coulter Inc., Brea, CA, USA) and commercial kits (Sino-German Beijing Leadman Biotech Ltd., Beijing, China).

Cui Z., Wang X., Hou Z., Liao S., Qi M., Zha A., Yang Z., Zuo G., Liao P., Chen Y, & Tan B. (2020). Low-Protein Diet Supplemented with Medium-Chain Fatty Acid Glycerides Improves the Growth Performance and Intestinal Function in Post-Weaning Piglets. Animals : an Open Access Journal from MDPI, 10(10), 1852.

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Serum Enzyme Analysis Protocol

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The concentrations of serum aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), alkaline phosphatase (AKP), and glutamyl transpeptidase (GGT) were analyzed using the Biochemical Analytical Instrument (Beckman CX4) and commercial kits (Sino-German Beijing Leadman Biotech Ltd., Beijing, China).

Duan Y., Song B., Zheng C., Zhong Y., Guo Q., Zheng J., Yin Y., Li J, & Li F. (2021). Dietary Beta-Hydroxy Beta-Methyl Butyrate Supplementation Alleviates Liver Injury in Lipopolysaccharide-Challenged Piglets. Oxidative Medicine and Cellular Longevity, 2021, 5546843.

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Serum Biochemical Analysis

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Serum biochemical parameters were determined by the Biochemical Analytical Instrument (Beckman CX4) according to the instructions of manufacturer. And corresponding kits were bought from Roche (Shanghai, China).

Zhao Y., Wang J., Wang H., Huang Y., Qi M., Liao S., Bin P, & Yin Y. (2020). Effects of GABA Supplementation on Intestinal SIgA Secretion and Gut Microbiota in the Healthy and ETEC-Infected Weanling Piglets. Mediators of Inflammation, 2020, 7368483.

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Serum Lipid Profile Analysis

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Serum samples were separated after centrifugation at 3000 rpm for 10 min under 4 °C. Serum glucose, high density lipoprotein (HDL), low density lipoprotein (LDL), triacylglycerol (TG), and total cholesterol (CHOL) were measured using the Biochemical Analytical Instrument (Beckman CX4, Beckman Coulter, Germany) and commercially available kits from Roche (Shanghai, China).

Zhong Y., Song B., Zheng C., Zhang S., Yan Z., Tang Z., Kong X., Duan Y, & Li F. (2020). Flavonoids from Mulberry Leaves Alleviate Lipid Dysmetabolism in High Fat Diet-Fed Mice: Involvement of Gut Microbiota. Microorganisms, 8(6), 860.

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