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4 protocols using ethanol

1

Development of Stable Sunflower Oil Emulsions

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Sunflower oil (Solesta, Aldi, Fermoy, Ireland), purchased in a local supermarket, was used as oil phase for preparation of double emulsions (DEs). The lipophilic emulsifier used to stabilize the colloidal emulsions, polyglycerol polyricinoleate (PGPR), was supplied by Danisco (Copenhagen, Denmark), while whey protein isolates (WPI), used as hydrophilic emulsifiers in outer aqueous phase and consisted of 95% β-lactoglobulin, were from Davisco Foods International (Le Sueur, MN, USA). Maltodextrins with average dextrose equivalent values of 10 and 21 (MDE 10 and MDE 21) were obtained from Grain Processing Corporation (Muscatine, IA, USA). Deionised water was used for preparation of solutions. Folin-Ciocalteu reagent, sodium carbonate and formic acid were supplied by Kemika (Zagreb, Croatia), while ethanol was from Carlo-Erba (Peypin, France); all chemicals were of analytical grade. Gallic acid and HPLC standards, ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt], DPPH (2,2-diphenyl-1-picrylhydrazyl), Trolox (6-hydroxy--2,5,7,8-tetramethylchromane-2-carboxylic acid), and Nile blue were obtained from Sigma-Aldrich (Steinheim, Germany). Acetonitrile and methanol (HPLC grade) were purchased from J.T.Baker (Deventer, the Netherlands).
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2

Synthesis of Chalcogenide Nanocrystals

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Antimony acetate (Sb(OAc)3, 99.99% metal basis, Aldrich), bismuth acetate (Bi(OAc)3, 99.999% metal basis, Aldrich), selenium powder (99.99% Strem Chemicals), tellurium powder (99.997% (trace metals basis), Alfa Aesar), Na2S nonahydrate (99,99%, Aldrich), Oleic acid (90% technical grade, Aldrich), Trioctylphosphine (TOP 380, 98%, Cytec), octadecene (ODE, 90% technical grade, Aldrich), hexane (95% RPE-ACS, Carlo Erba), ethanol (anhydrous 99.9%, Carlo Erba), N-methyl formamide (NMFA, 99%, Alfa Aesar.)
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3

Lycopene Extraction from Tomato Peels

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Acetone (CAS No. 67-64-1), methanol (CAS No. 67-56-1), ethanol (CAS 64-17-5), ethyl acetate (CAS No. 141-78-6), ethyl lactate (CAS No. 687-47-8) and n-hexane (CAS No. 110-54-3) were obtained from Carlo Erba (Milano, Italy). Butylated hydroxytoluene (BHT, CAS No. 128-37-0), potassium persulfate (CAS No. 7727-21-1), Trolox [6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid] (CAS No. 53188-07-1) and ABTS [2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt] (CAS No. 30931-67-0) were provided by Sigma–Aldrich (St. Louis, Mo, USA). All chemicals were of analytical grade and used as received.
Fresh ripe tomatoes of the variety Piccadilly were purchased from a local market and stored in the dark at 4 °C. Before each set of experiments, a few tomato fruits were immersed in boiling water for 1–2 min, cooled in tap water and hand peeled. Tomato peels were left to dry in air for about 2 h and then characterized for total lycopene and moisture content.
Natural lycopene standard (10% by weight) was obtained from LycoRed Natural Products Industries Ltd. (Beer-Sheva, Israel).
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4

Synthesis of Na-BA Lipid Compound

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Na-BA was purchased from Nu-Chek Prep, Inc. (Elysian, U.S.A.). PVA 9000, acetonitrile, DMF, dioxane, DMSO, lauric acid, THF, trifluoroacetic acid and dichloromethane from Sigma (Dorset, UK); hydrochloric acid, sodium hydroxide and monobasic sodium phosphate from Merck (Darmstadt, Germany); methanol, thionyl chloride, diisopropyl ether, diethyl ether and ethanol from Carlo Erba (Val De Reuil, France); deionized water was obtained by a MilliQ system (Millipore, Bedford, MO).
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