After treated with the indicated concentrations of KA for 48 h, 8505C, TPC-1, K1, C643 cells were harvested, washed with PBS, and subjected to sequential staining with Annexin V-FITC/PI Detection Kit (Roche Applied Science, Penzberg, Germany) by flow cytometer according to the manufacturer’s protocol. Early apoptotic cells show Annexin V-FITC+/PI− staining patterns, whereas late apoptotic cells exhibit Annexin V-FITC+/PI+ staining patterns. They were collectively called apoptotic cells. Each experiment was performed in triplicate.
Annexin 5 fitc pi detection kit
Manufactured by Roche
Sourced in Germany
The Annexin V-FITC/PI Detection Kit is a laboratory product that allows for the detection and quantification of apoptotic cells. It combines Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a DNA-binding dye. This combination enables the identification of viable, early apoptotic, and late apoptotic/necrotic cells through flow cytometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using annexin 5 fitc pi detection kit
1
Quantifying Apoptosis in Cancer Cells
2
Annexin V FITC PI Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The transfected cells were adhered to the wall and washed two times with pre-cooled 1X DPBS, and the cells were mixed up in 1X binding buffer (100 μL). Then, Annexin V FITC PI detection kit (Roche Applied Science, Germany) was used for cell staining according to the instructions. Analysis of apoptosis was applied with a flow cytometer (BD Biosciences, USA).
3
Apoptosis Detection in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
8505C, TPC1, K1 and C643 cells were treated with the indicated concentrations of KA for 48 h. Cells were then harvested, washed with PBS, and subjected to sequential staining with Annexin V-FITC/PI Detection Kit (Roche Applied Science, Penzberg, Germany) by flow cytometer according to the manufacturer's protocol.
Early apoptotic cells show Annexin V-FITC+/PI-staining patterns, whereas late apoptotic cells exhibit Annexin V-FITC+/PI+ staining patterns. They were collectively called apoptotic cells. Each experiment was performed in triplicate.
Early apoptotic cells show Annexin V-FITC+/PI-staining patterns, whereas late apoptotic cells exhibit Annexin V-FITC+/PI+ staining patterns. They were collectively called apoptotic cells. Each experiment was performed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!