Rabbit anti ncl

Manufactured by Abcam

Rabbit anti-NCL is a primary antibody that recognizes the nucleolin (NCL) protein. Nucleolin is a multifunctional protein involved in various cellular processes, including ribosome biogenesis, chromatin remodeling, and regulation of gene expression. This antibody can be used to detect and study the NCL protein in a variety of applications.

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Lab products found in correlation

Hrp conjugated goat anti mouse or goat anti rabbit secondary antibodies, by Southern Biotech (2 mentions) Mouse anti ncl, by Santa Cruz Biotechnology (1 mentions) Mouse anti hnrnpc1 c2, by Abcam (1 mentions) Rabbit anti h3, by Cell Signaling Technology (1 mentions) Mouse anti strep, by Qiagen (1 mentions) Rabbit anti flag, by Merck Group (1 mentions) Rabbit anti eif4h, by Cell Signaling Technology (1 mentions) Rabbit anti actin, by Abcam (1 mentions) Rabbit anti ythdc2, by Abcam (1 mentions) Rabbit anti est1a smg6, by Abcam (1 mentions) Rabbit anti hur, by Merck Group (1 mentions) Mouse anti gapdh, by Abcam (1 mentions)

Close spelling variants of this product

Anti-NCL Rabbit anti-

2 protocols using rabbit anti ncl

Western Blot Analysis of Nuclear Proteins

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Cell lysates were prepared in lysis buffer and quantified by Bradford assay. Equivalent amounts of each sample were resolved by SDS-PAGE and Western blotted with the following antibodies: Rabbit anti-NCL (Abcam), Mouse anti-NCL (Santa Cruz), Rabbit anti-eIF4H (Cell signaling). Rabbit anti-Flag (Sigma), Mouse anti hnRNPC1/C2 (Abcam), Rabbit anti-H3 (Cell Signaling), Rabbit anti-Xrn1 (Sigma), Mouse anti-Strep (Qiagen). Primary antibodies were followed by HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (Southern Biotechnology, 1:5000).

Muller M., Hutin S., Marigold O., Li K.H., Burlingame A, & Glaunsinger B.A. (2015). A Ribonucleoprotein Complex Protects the Interleukin-6 mRNA from Degradation by Distinct Herpesviral Endonucleases. PLoS Pathogens, 11(5), e1004899.

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Comprehensive Western Blot Analysis of RBP Interactome

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Lysates were resolved by SDS-PAGE and western blotted with the following antibodies at 1:1000 in TBST (Tris-buffered saline, 0.1% Tween 20): rabbit anti-EST1A/SMG6 (Abcam), rabbit anti-NCL (Abcam), rabbit anti-HuR (Millipore), rabbit anti-actin (Abcam), rabbit anti-IGF2BP1 (Abcam), rabbit anti-YTHDC2 (Abcam), rabbit anti-hnRNPU (Abcam), rabbit anti-ZC3HAV1 (Abcam), rabbit anti-hnRNPD (Abcam), rabbit anti-STAU1 (Pierce), mouse anti-NPM1 (Abcam), rabbit anti-GADD45 (Abcam), mouse anti-GAPDH (Abcam), mouse anti-ORF59 (Adv biotechnologies), or rabbit anti-K8.1 (PRF&L, inc). Primary antibody incubations were followed by HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (Southern Biotechnology, 1:5000).

Muller M, & Glaunsinger B.A. (2017). Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases. PLoS Pathogens, 13(8), e1006593.

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