The vector for CHC2-YFP was constructed as follows: Arabidopsis genomic DNA was used as a template with primers CHC2-Pentr-F and CHC2-Pentr-R to amplify the whole sequence of CHC2. The final PCR product was cloned into pENTR/DTOPO (Life Technologies), and then into the destination vector pEarleyGate 101 by LR reactions (Life Technologies). For the YFP-FLOT1 construct, the full-length CDS of FLOT1 was cloned into the pENTR vector (Life Technologies), then into the destination vector pEARLYGATE 104 for YFP tagging (Life Technologies). For the TET8-CFP construct, the full-length CDS of TET8 was cloned into the pENTR vector (Life Technologies), then into the destination vector pEARLYGATE 102 for CFP tagging (Life Technologies). For the construct to express YFP, the YFP sequence was amplified from pEARLYGATE 101 and then cloned into pENTR vector and finally into pEARLYGATE 100 (Life Technologies). Primer sequences are listed in Supplementary Data 1 .
Pearlygate 104
Manufactured by Thermo Fisher Scientific
The PEARLYGATE 104 is a laboratory equipment device designed for scientific research and analysis. It is a high-performance instrument that serves as a core function for specific laboratory applications. The product details and technical specifications are available upon request.
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Lab products found in correlation
Pearleygate101, by Thermo Fisher Scientific (2 mentions)
Pearlygate 102, by Thermo Fisher Scientific (2 mentions)
Pearlygate 101, by Thermo Fisher Scientific (2 mentions)
Pearlygate 100, by Thermo Fisher Scientific (2 mentions)
Pentr d topo, by Thermo Fisher Scientific (1 mentions)
Pentr vector, by Thermo Fisher Scientific (1 mentions)
Pentr sd d topo, by Thermo Fisher Scientific (1 mentions)
Pentr sd d topo vector, by Thermo Fisher Scientific (1 mentions)
2 protocols using pearlygate 104
1
Construction of Fluorescently-Tagged Protein Vectors
2
Cloning Fluorescently Tagged Proteins
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The vector for CHC2-YFP was constructed as follows: Arabidopsis genomic DNA was used as a template with primers CHC2-Pentr-F and CHC2-Pentr-R to amplify the whole sequence of CHC2. The final PCR product was cloned into pENTR/SD/D-TOPO (Life Technologies), and then into the destination vector pEarleyGate 101 by LR reactions (Life Technologies). For the YFP-FLOT1 construct, the full-length CDS of FLOT1 was cloned into the pENTR/SD/D-TOPO vector (Life Technologies), then into the destination vector pEARLYGATE 104 for YFP tagging (Life Technologies). For the TET8-CFP construct, the full-length CDS of TET8 was cloned into the pENTR/SD/D-TOPO vector (Life Technologies), then into the destination vector pEARLYGATE 102 for CFP tagging (Life Technologies). For the construct to express YFP, the YFP sequence was amplified from pEARLYGATE 101 and then cloned into pENTR/SD/D-TOPO vector and finally into pEARLYGATE 100 (Life Technologies). Primer sequences are listed in Supplementary Data 1 .
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