Salmonella typhimurium

Manufactured by China General Microbiological Culture Collection Center

Sourced in China

Salmonella typhimurium is a bacterial strain used for laboratory research and testing. It is a Gram-negative, rod-shaped bacterium that is classified under the genus Salmonella. The strain is commonly used in microbiology and biotechnology applications to study bacterial pathogenesis, host-pathogen interactions, and the development of diagnostic and therapeutic methods.

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4 protocols using salmonella typhimurium

Microbial Culture and Plasmid Acquisition

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EcN, E. faecalis, S. aureus, and Salmonella typhimurium were purchased from China general microbiological culture collection center (GMCC, China), cell lines (CT26 and 4T1) were obtained from American Type Culture Collections (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (Sigma, USA) supplemented with 10% (v/v) inactivated FBS (Sigma, USA) and 1% (v/v) antibiotic/anti-mycotic solution (Sigma, USA) at 37 °C incubator with 5% CO₂. Regular mycoplasma evaluations were performed of the cell culture environment to ensure the absence of mycoplasma contamination. Plasmids pBBR1MCS2-Tac-mCherry, pBBR1MCS2-Tac-GFP, pMD18-luxCDABE and all other reagents were purchased from domestic suppliers and used as received.

Cao Z., Cheng S., Wang X., Pang Y, & Liu J. (2019). Camouflaging bacteria by wrapping with cell membranes. Nature Communications, 10, 3452.

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Characterizing Microbiome-Host Interactions

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EcN and Salmonella typhimurium were purchased from China General Microbiological Culture Collection Center. Caco-2 and Raji cell lines were obtained from the American Type Culture Collection. Baker’s yeast was obtained from Lesaffre. Minimum Eagle’s medium (MEM), RPMI 1640, fetal bovine serum, and antibiotic/antimycotic solution were purchased from Thermo Fisher Scientific. Calcofluor-white stain was purchased from Sigma-Aldrich. The β-Glucan assay kit was purchased from Megazyme. Fluorescence-activated cell sorting antibodies including anti-mouse CD3-PerCP Cy5.5, CD4–fluorescein isothiocyanate (FITC), CD8–allophycocyanin (APC), CD11c-FITC, CD80–phycoerythrin (PE), CD86-APC, CD45R/B220-PE-Cy7, and CD138-APC were purchased from eBioscience. IgA-PE was purchased from Thermo Fisher Scientific. Plasmids pBBR1MCS2-Tac-mCherry (kanamycin resistance) and all other reagents were purchased from domestic suppliers and used as received.

Lin S., Mukherjee S., Li J., Hou W., Pan C, & Liu J. (2021). Mucosal immunity–mediated modulation of the gut microbiome by oral delivery of probiotics into Peyer’s patches. Science Advances, 7(20), eabf0677.

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Bacterial Culture Acquisition Protocol

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Escherichia coli JM109 strain was obtained from the School of Life Science, Huzhou Univesity. S. aureus (1.282), Bacillus subtilis (1.15792) and Salmonella typhimurium (1.1190) were purchased from China General Microbiological Culture Collection Center (CGMCC). The sterile defibrinated sheep blood was obtained from Pingrui Biotechnology (Beijing) Co., Ltd.

Jiang X., Qian K., Liu G., Sun L., Zhou G., Li J., Fang X., Ge H, & Lv Z. (2019). Design and activity study of a melittin–thanatin hybrid peptide. AMB Express, 9, 14.

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Antimicrobial Susceptibility of Clinical Isolates

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Escherichia coli (E. coli) CCTCC AB 2012883, E. coli CCTCC AB 2018675, Shigella dysenteriae (S. dysenteriae) CGMCC 1.1869, Proteus vulgaris (P. vulgaris) CGMCC 1.1651, Salmonella typhimurium (S. typhimurium) CGMCC 1.1174, and Staphylococcus albus (S. albus) CGMCC 1.3374 were obtained from the China General Microbiological Culture Collection Center. Staphylococcus aureus (S. aureus) CMCC 26003 was acquired from National Center for Medical Culture Collections. Staphylococcus citreus (S. citreus) CCTCC AB 91115 was acquired from China Center for Type Culture Collection.
All clinical isolates tested in this study were collected from the Affiliated Nanhua Hospital, University of South China, during the period between September in 2021 and May in 2022. E. faecium, S. aureus, K. pneumoniae, A. baumannii, P. aeruginosa, and E. species clinical multidrug-resistant (MDR) strains isolates were collected from patients’ secretions, urine, sputum, or blood. All bacteria were incubated in Luria-Bertani (LB) medium and Mueller–Hinton broth (MHB) at 37 °C at 200 rpm to logarithmic growth phase and diluted to the desired inoculum concentration, based on the optical density at 600 nm. Azithromycin (AZI), erythromycin (ERY), and doxycycline (DOX) were purchased from APExBIO. All other reagents were obtained from commercial sources and were of analytical grade.

Tan H., Wang J., Song Y., Liu S., Lu Z., Luo H, & Tang X. (2022). Antibacterial Potential Analysis of Novel α-Helix Peptides in the Chinese Wolf Spider Lycosa sinensis. Pharmaceutics, 14(11), 2540.

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