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Ek0392

Manufactured by Boster Bio
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Sourced in China

The EK0392 is a piece of lab equipment designed for performing enzyme-linked immunosorbent assay (ELISA) testing. It is a microplate reader that can measure the absorbance of samples in a 96-well microplate format, which is a common method used in ELISA protocols.

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Lab products found in correlation

Epoch 2, by Agilent Technologies (1 mentions) Ek0410, by Boster Bio (1 mentions) Ek0525, by Boster Bio (1 mentions) Ek0416, by Boster Bio (1 mentions) Seb472hu, by Cloud-Clone (1 mentions) Sea079hu, by Cloud-Clone (1 mentions) Sea133hu, by Cloud-Clone (1 mentions)

4 protocols using ek0392

1

Quantification of IL-1β and IL-18 by ELISA

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Supernatant was collected for the measurement of IL-1β and IL-18 concentration using ELISA kits (EK0864 and EK0392; Boster, Wuhan, China) according to manufacturer's instructions. Briefly, the supernatant of cell cultures was collected and IL-1β and IL-18 were determined by ELISA. The optical density at 450 nm was determined using the microplate reader Epoch 2 (BioTek).
Tong L., Xie C., Wei Y., Qu Y., Liang H., Zhang Y., Xu T., Qian X., Qiu H, & Deng H. (2019). Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release. Frontiers in Oncology, 9, 364.
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2

Cytokine Quantification by ELISA

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The concentration levels of IL-1β, IL-6, IL-10 and TNF-α in the cell culture supernatants were measured using ELISA kits (EK0392, EK0410, EK0416, EK0525, Boster Biological Technology, China) according to the manufacturers’ protocols.
Li S., Li A., Zhai L., Sun Y., Yu L., Fang Z., Zhang L., Peng Y., Zhang M, & Wang X. (2022). Suppression of FPR2 expression inhibits inflammation in preeclampsia by improving the biological functions of trophoblast via NF-κB pathway. Journal of Assisted Reproduction and Genetics, 39(1), 239-250.
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3

Quantifying Bone Marker Profiles

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Proinflammatory cytokines and protein analysis of main bone markers produced by cells within the 3D model were measured by ELISA colorimetric tests for the quantification of Interleukin 6 (IL6, SEA079Hu Cloud-Clone Corp, USA), Interleukin 1ß (IL1ß, EK0392, Boster Bio, Pleasanton, CA, USA), Tumor Necrosis Factor α (TNFα, SEA133Hu, Cloud-Clone Corp., Katy, TX, USA), Collagen alpha-1(I)chain (COLL1a1, SEA350Hu Cloud-Clone Corp., Katy, TX, USA) and Alkaline Phosphatase (ALP, SEB472Hu Cloud-Clone Corp., Katy, TX, USA). Supernatants were collected from all wells at all experiment times and maintained at −20 °C until used.
Tschon M., Boanini E., Sartori M., Salamanna F., Panzavolta S., Bigi A, & Fini M. (2022). Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model. International Journal of Molecular Sciences, 23(11), 5988.
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4

Cytokine Measurement via ELISA

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An enzyme-linked immunosorbent assay (ELISA) kit for human IL-1β (Boster, EK0392, Beijing, China) and IL-18 (Boster, EK0864, Beijing, China) was purchased from Boster. Supernatants (500 μL) were collected from each group, and ELISAs were performed according to the instructions provided with the ELISA kit. Cytokine concentrations in individual samples were quantified by measuring absorbance at 450 nm with a microplate reader.
Li M., Liu Y., Nie X., Ma B., Ma Y., Hou Y., Yang Y., Xu J, & Wang Y. (2023). S100A4 Promotes BCG-Induced Pyroptosis of Macrophages by Activating the NF-κB/NLRP3 Inflammasome Signaling Pathway. International Journal of Molecular Sciences, 24(16), 12709.
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