Phospho pkcα β 2 thr638 641

Manufactured by Cell Signaling Technology

Sourced in United States

Phospho-PKCα/β II (Thr638/641) is a lab equipment product that detects phosphorylation of protein kinase C alpha (PKCα) and protein kinase C beta II (PKCβII) at threonine residues 638 and 641, respectively. This product is used to measure the activation state of these important signaling proteins.

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Lab products found in correlation

Propidium iodide, by Thermo Fisher Scientific (3 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Trypan blue solution, by Merck Group (1 mentions) Cep 1347, by Bio-Techne (1 mentions) Phospho pkcδ thr505, by Cell Signaling Technology (1 mentions) Marcks, by Cell Signaling Technology (1 mentions) Phospho pkc pan βii ser660, by Cell Signaling Technology (1 mentions) G 6976, by Merck Group (1 mentions) P53 antibody sc 126, by Santa Cruz Biotechnology (1 mentions) Af1244, by R&D Systems (1 mentions) Crystal violet, by Merck Group (1 mentions) Phospho akt thr450, by Cell Signaling Technology (1 mentions) Histone h3, by Active Motif (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Krt10, by Fortrea (1 mentions)

2 protocols using phospho pkcα β 2 thr638 641

Molecular Signaling Pathway Inhibitors

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CEP-1347 was purchased from TOCRIS Bioscience (Bristol, UK). Gö6976, Crystal Violet (C.I. 42555), and Trypan Blue solution (0.4%) were obtained from Merck KGaA (Darmstadt, Germany). Propidium iodide and Hoechst33342 were supplied by Thermo Fisher Scientific (Waltham, MA, USA). An antibody against MDM4 (A700-000-T) was purchased from BETHYL (FORTIS LIFE SCIENCES, Waltham, MA, USA). An antibody against MDM2 (AF1244) was purchased from R&D Systems (Minneapolis, MN, USA). A p53 antibody (sc-126) was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Antibodies against GAPDH (#5174), p21 Waf1/Cip1 (cyclin-dependent kinase inhibitor 1A, CDKN1A) (#2947), Myristoylated alanine-rich protein kinase C (PKC) substrate (MARCKS) (#5607), Phospho-MARCKS (Ser152/156) (#2741), Phospho-PKC (pan) (βII Ser660) (#9371), Phospho-PKCα/β II (Thr638/641) (#9375), Phospho-PKCδ (Thr505) (#9374), and Phospho-PKCθ (Thr538) (#9377) were from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Togashi K., Suzuki S., Mitobe Y., Nakagawa-Saito Y., Sugai A., Takenouchi S., Sugimoto M., Kitanaka C, & Okada M. (2023). CEP-1347 Dually Targets MDM4 and PKC to Activate p53 and Inhibit the Growth of Uveal Melanoma Cells. Cancers, 16(1), 118.

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Comprehensive Antibody Panel for Protein Kinase Analysis

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The antibodies used in this study included PKN1 (BD Biosciences, San Jose, CA, #610686, Santa Cruz, Dallas, TX, sc-1842), custom anti-PKN1 antibody (residues 105–115; cATHDGPQSPGA), PKN2 (BD Biosciences #610794, Epitomics, Cambridge, MA, #1498-1), phospho-PKN1(Thr774)/PKN2(Thr816) (Cell Signaling Technology, Danvers, MA, #2611), phospho-PKN1(Ser916) (custom-made against peptide CEAPTLpSPPRD), M2 (Sigma F3165), Histone H3 (Active Motif, Carlsbad, CA, #39163, Cell Signaling Technology #2650 and Abcam, Cambridge, MA, ab1791), phospho-H3(Thr6) (Abcam ab14102), phospho-H3(Ser10) (Active Motif #39253), phospho-H3(Thr11) (Cell Signaling Technology #9764 and Abcam ab5168), Phospho-Akt(Thr450) (Cell Signaling Technology #12178), Phospho-Akt(Ser473) (Cell Signaling Technology #4058), Akt (Cell Signaling Technology #9272), Phospho-PKCα/β II(Thr638/641) (Cell Signaling Technology #9375), PKC (Cell Signaling Technology #2056), E-Cadherin (Cell Signaling Technology #3195), Krt5 (Covance, Princeton, NJ, SIG-3475), Krt8 (Covance MMS-162P), Krt10 (Covance PRB-159P), and Ki67 (Abcam ab16667).

Yang C.S., Melhuish T.A., Spencer A., Ni L., Hao Y., Jividen K., Harris T., Snow C., Frierson H., Wotton D, & Paschal B.M. (2017). The Protein Kinase C Super-family Member PKN is Regulated by mTOR and Influences Differentiation During Prostate Cancer Progression. The Prostate, 77(15), 1452-1467.

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