HT-22 cells (ZQ0476, Shanghai Zhongqiao Xinzhou Biotechnology Co., Ltd) were used for the in vitro study. The cells were placed in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 100 μ/mL penicillin and 100 ng/mL streptomycin (HY-B0472, MCE) and maintained at 37°C under 5% CO2. The cells were seeded in 6-well plates and incubated with different drugs treatment: 100 µM perampanel [21 (link)], 100 μM SQ22536 (HY-100396, MedChemExpress), a adenylate cyclase (AC) inhibitor; 10 µM forskolin (HY-15371, MedChemExpress), a AC agonist; 10 µM H-89 (HY-15979, MedChemExpress), a protein kinase A (PKA) inhibitor; 0.1 mM 8-bromo-cAMP sodium salt (HY-12306, MedChemExpress), a PKA agonist and 25 µM KG501 (HY-103299, MedChemExpress), and a cAMP-responsive-element-binding protein (CREB) inhibitor for 24 hours. Cell lysates were collected to do western blot analysis 24 hours later.
Hy 15371
Manufactured by MedChemExpress
HY-15371 is a laboratory instrument used for analytical measurements. It is designed to perform precise and reliable measurements for various applications in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hy 15371
1
Drug Treatments Modulate HT-22 Cell Signaling
2
Evaluating PDE4D Inhibitor GEBR32a on cAMP
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To evaluate the effect of PDE4D inhibition by GEBR32a on global intracellular cAMP levels, N2a and HT22 cells were seeded in 12-well plates at 106 cells per well. Cells were treated with DMSO or GEBR32a 0.01–3 µM (n ≥ 4/condition) and 1 µM of the adenylyl cyclase activator forskolin (MedChemExpress, HY-15371) for 1 h and subsequently lysated in 0.1 M HCl. Concentrations of cAMP were measured using the Cyclic AMP ELISA Kit (Cayman Chemical Company, 581,001) according to manufacturer’s instructions. Results are depicted as fold change over DMSO control.
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