Phospholipase A1 (PLA1) from Thermomyces lanuginosus expressed in Aspergillus oryzae was purchased from Sigma-Aldrich Co. (Tokyo, Japan). Phospholipase A1 from Aspergillus oryzae (10,000–13,000 U/g) was purchased from Mitsubishi Kagaku Foods Co. (Tokyo, Japan). Glycerophospholipid specific phospholipase D (GPL-PLD) from Streptmyces sp. (41.1 u/mg) was purchased from Asahi Kasei Pharma Co. (Tokyo, Japan), tyramine oxidase from Arthrobacter sp. (4.6 u/mg) and choline oxidase from Arthrobactor glob. (20 u/mg) were purchased from Asahi Kasei Pharma Co. (Tokyo, Japan). Horseradish peroxidase (460 u/mg) was obtained from Oriental Yeast Co. (Tokyo, Japan). Amplex Red reagent was obtained from Molecular Probes Inc. (Eugene, Oregon, USA). Phosphatidylethanolamine from egg yolk and phosphatidylcholine from bovine liver were obtained from Doosan Serdary Laboratory (Toronto, Canada). Scallop and chicken breast muscle were obtained at a market place.
Horseradish peroxidase
Manufactured by Oriental Yeast
Sourced in Japan
Horseradish peroxidase is an enzyme commonly used in various laboratory applications. It catalyzes the oxidation of substrates in the presence of hydrogen peroxide. The core function of horseradish peroxidase is to serve as a detection agent in various biochemical assays and immunoassays.
Automatically generated - may contain errors
2 protocols using horseradish peroxidase
1
Enzymatic Lipid Quantification Protocol
2
Enzymatic Lipid Quantification Techniques
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Choline oxidase and lipoprotein lipase were obtained from Wako Pure Chemical Industries (Osaka, Japan). Phospholipase D and glycerophospholipid-specific phospholipase D were purchased from Biomol International (Plymouth meeting, PA). Amine oxidase was provided by Asahi Kasei Pharma (Tokyo, Japan). l -Amino acid oxidase, l -glycerol-3-phosphate oxidase, and sphingomyelinase were obtained from Worthington (Lakewood, NJ), Roche Diagnostics (Mannheim, Germany), and Sigma-Aldrich (St. Louis, MO), respectively. Calf intestine alkaline phosphatase and horseradish peroxidase were obtained from Oriental Yeast (Osaka, Japan). Purified phosphatidylcholine (PC), phosphatidic acid (PA), L-α-palmitoyl-oleoyl phosphatidylethanolamine (PE), phosphatidylserine (PS), and sphingomyelin (SM) were purchased from Avanti Polar lipids (Alabaster, AL). The fluorescent probes Amplex Red (N-acetyl-3,7-dihydroxyphenoxazine) and Stop Reagent were purchased from Thermo Fisher Scientific (Waltham, MA).
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