Dna finger printing

The human THP1 monocytic cell line was purchased from ATCC and authenticated by DNA finger printing (Eurofins). The murine lung cancer cell line LLC1 was a gift from Angelica Loskog (Uppsala University) and the mouse breast cancer cell line EO771 was obtained from Maria Ulvmar (Uppsala University). The mouse neuroblastoma cell line 9464D was initially established from a transgenic mouse model on the C57BL/6 background that overexpressed the TH-MYCN oncogene and was obtained from Malin Wickström (Karolinska Institute, Stockholm, Sweden). The Ret melanoma cell line was established from a transgenic mouse model developing spontaneous melanoma (16 (link)) and was a gift from Viktor Umansky from DKFZ in Heidelberg, Germany. All cell lines were maintained in the IMDM medium (Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin (Thermo Fisher Scientific) and tested for mycoplasma contamination (MycoAlert, Lonza).

Human breast cancer cell line, MDA-MB-231 (92020424, Sigma Aldrich), and HEK293T cells (CRL-3216, American Type Culture Collection, ATCC) were purchased. HCC1937 and BT549 cell lines were a gift from Dr. Óscar Fernández-Capetillo (Karolinska Institutet, Sweden). Mouse breast cancer cell line EO771 was kindly provided by Dr. Maria Ulvmar (Uppsala University, Sweden). Unless otherwise stated, all cell lines were maintained in IMDM medium (Thermo Fisher Scientific) containing 10% heat-inactivated Fetal Bovin Serum (FBS) and 1% penicillin-streptomycin solution (Thermo Fisher Scientific) at 37 °C with 5% carbon dioxide. Cell lines were authenticated using DNA fingerprinting (Eurofins) and checked for mycoplasma infection routinely (MycoAlert, Lonza).