Cultured keratinocytes or epidermis sheets were lysed in radioimmunoprecipitation assay buffer supplemented with protease and phosphatase inhibitor cocktail (Thermo Scientific, #78440). Mouse anti-actin Ab (1:3,000; Cell Signaling Technology, #3700S), rabbit anti-ppp6c Ab (1:1,000; Abcam, #EPR8764), rat anti-p65 Ab (Cell Signaling Technology, #8242S) and rabbit anti-mouse Ki67 Ab (eBioscience, #42–5698) were used. Data was analysed using Image Lab software (Bio-Rad). Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 13 .
Mouse anti actin ab
Manufactured by Cell Signaling Technology
Mouse anti-actin Ab is a primary antibody that specifically recognizes actin, a ubiquitous cytoskeletal protein found in all eukaryotic cells. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and quantify actin levels in biological samples.
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Lab products found in correlation
2 protocols using mouse anti actin ab
1
Western Blot Analysis of Cultured Keratinocytes
2
Immunoblotting of Cultured T Cells and Mouse CNS
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Cultured T cells and the CNS of mice were lysed in radio immunoprecipitation assay buffer supplemented with protease and phosphatase inhibitor cocktail (Thermo Scientific). Mouse anti-actin Ab (1:3,000) (Cell Signaling Technology), rabbit anti-Gprc5a Ab (1:1,000) (Dr Jiong Deng provided) were used. The signal was detected with Pierce ECL Western Blotting Substrate (Thermo Scientific) and GE ImageQuant LAS 4000 (GE Healthcare). Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 8 .
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