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400 ft nmr spectrometer

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The Agilent -400 FT-NMR spectrometer is a laboratory instrument designed for nuclear magnetic resonance (NMR) spectroscopy. It is used to analyze the structure and composition of chemical samples by detecting the magnetic properties of atomic nuclei within the sample.

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Lab products found in correlation

2414 refractive index detector, by Waters Corporation (6 mentions) 717 plus autosampler, by Waters Corporation (4 mentions) 515 hplc pump, by Waters Corporation (4 mentions) 1525 binary hplc pump, by Waters Corporation (1 mentions) Inveon preclinical imaging station, by Siemens (1 mentions) Zeta nanosizer, by Malvern Panalytical (1 mentions) Fts 185 spectrometer, by Bio-Rad (1 mentions)

10 protocols using 400 ft nmr spectrometer

1

Polymer Characterization by NMR and GPC

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1H NMR spectrum was examined on a Varian-400 FT-NMR spectrometer at 400.0 MHz with d6-DMSO as the solvent. Molecular weight (Mn and Mw) and distribution (Mw/Mn) of the synthesized polymers were measured by gel permeation chromatography (GPC) performed on a Waters 515 HPLC pump and a Waters 717 Plus Autosampler equipped with a Waters 2414 refractive index detector. Tetrahydrofuran (THF) was used as the eluent with a flowing rate of 1.0 mL/min at 35°C. A series of commercial polystyrene standards with narrow molecular weight distribution were applied to calibrate the GPC elution traces.
Luo Z., Xu J., Sun J., Huang H., Zhang Z., Ma W., Wan Z., Liu Y., Pardeshi A, & Li S. (2020). Co-delivery of 2-Deoxyglucose and a Glutamine Metabolism Inhibitor V9302 via a Prodrug Micellar Formulation for Synergistic Targeting of Metabolism in Cancer. Acta biomaterialia, 105, 239-252.
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2

Synthesis of PMet-P(cdmPEG2K) Polymer

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PMet-P(cdmPEG2K) was synthesized by a ring opening reaction of PEG2K-cdm and pMet polymers. Boc-deprotected PMet (compound 5, 100 mg) and PEG2K-cdm (compound 1, 303 mg) were dissolved in 4 mL of DMSO and stirred at 37 °C for 24 h. The mixture was dialyzed against DMSO and then distilled water for 2 days. The final product of PMet-P(cdmPEG2K) polymer was then lyophilized.
1H NMR spectrum was analyzed on a Varian-400 FT-NMR spectrometer at 400 MHz with DMSO-d6 and CDCl3 as the solvent.
Liu Y., Sun J., Huang Y., Chen Y., Li J., Liang L., Xu J., Wan Z., Zhang B., Li Z, & Li S. (2021). Metformin-conjugated micellar system with intratumoral pH responsive de-shielding for co-delivery of doxorubicin and nucleic acid. Biochemical pharmacology, 189, 114453.
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3

1H NMR and GPC Characterization of Polymers

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1H NMR spectrum was conducted on a Varian 400 FT-NMR spectrometer at 400.0 MHz with CDCl3 as the solvent. Molecular weight (Mn and Mw) and polydispersity index (Mw/Mn) of the synthesized polymers were measured by gel permeation chromatography (GPC) equipped with a Waters 2414 refractive index detector, a Waters 515 HPLC pump and a Waters 717 Plus Autosampler. THF was used as the eluent with a flowing rate of 1.0 mL/min at 35°C. A series of commercial polystyrene standards with narrow molecular weight distribution were applied to calibrate the GPC elution traces.
Sun J., Chen Y., Li K., Huang Y., Fu X., Zhang X., Zhao W., Wei Y., Xu L., Zhang P., Venkataramanan R, & Li S. (2016). A Prodrug Micellar Carrier Assembled from Polymers with Pendant Farnesyl Thiosalicylic Acid Moieties for Improved Delivery of Paclitaxel. Acta biomaterialia, 43, 282-291.
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4

Characterization of Synthesized Polymers

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1H NMR spectrum was conducted on a Varian 400 FT-NMR spectrometer at 400.0 MHz with CDCl3 as the solvent. Molecular weight (Mn and Mw) and polydispersity index (Mw/Mn) of the synthesized polymers were determined by gel permeation chromatography (GPC) equipped with a Waters 2414 refractive index detector, a Waters 515 HPLC pump, and a Waters 717 Plus Autosampler. THF was used as the eluent with a flowing rate of 1.0 mL/min at 35°C. A series of polystyrene standards with narrow molecular weight distribution were used for calibration.
Li Z., Sun J., Huang Y., Liu Y., Xu J., Chen Y., Liang L., Li J., Liao Q., Li S, & Zhou K. (2018). A Nanomicellar Prodrug Carrier Based on Ibuprofen-Conjugated Polymer for Co-delivery of Doxorubicin. Frontiers in Pharmacology, 9, 781.
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5

Characterization of Synthesized Polymers

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1H NMR spectrum (400.0 MHz) was recorded on a Varian 400 FT-NMR spectrometer with CDCI3 as the solvent. Molecular weights (Mn and Mw) and molecular weight distributions (Mw/Mn) of the synthesized polymers were determined by gel permeation chromatography (GPC) equipped with a Waters 2414 refractive index detector. THF was used as the eluent with a flowing rate of 1.0 mL/min at 35°C. A series of polystyrene standards with narrow molecular weight distribution were applied for calibration. HPLC and FPLC were performed on a Waters 1525 Binary HPLC pump (Milford, MA) with a Waters 2489 UV/visible detector and a model 106 Bioscan radioactivity detector for the analysis of either 64Cu or 89Zr labeled conjugates using either a two-components buffer (0.1 v% TFA in de-ionized water + 0.1 v% TFA in acetonitrile) or PBS as the eluting phase for HPLC and FPLC respectively. PET/CT data were acquired using an Inveon Preclinical Imaging Station (Siemens Medical Solutions).
Sun J., Sun L., Li J., Xu J., Wan Z., Ouyang Z., Liang L., Li S, & Zeng D. (2018). A Multi-Functional Polymeric Carrier for Simultaneous Positron Emission Tomography Imaging and Combination Therapy. Acta biomaterialia, 75, 312-322.
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6

Characterization of Polymer Micelles

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1H NMR spectroscopy was performed on a Varian-400 FT-NMR spectrometer (400.0 MHz) with CDCl3 and DMSO-d6 as the solvent. The molecular weight (Mn and Mw) and distribution (Mw/Mn) of the synthesized polymers were measured by gel permeation chromatography (GPC) with a Waters 2414 refractive index detector. A series of polystyrene standards with a narrow molecular weight distribution were applied to calibrate the GPC elution traces. Tetrahydrofuran (THF) was used as the eluent at a flow rate of 1.0 mL/min at 35 °C, and a series of commercial polystyrene standards were used for the calibration. The size distribution and morphology of blank and drug-loaded micelles were examined by dynamic light scattering (DLS, Malvern Zeta Nanosizer) and transmission electron microscopy (TEM, negative staining method), respectively.
Sun J.J., Chen Y.C., Huang Y.X., Zhao W.C., Liu Y.H., Venkataramanan R., Lu B.F, & Li S. (2017). Programmable co-delivery of the immune checkpoint inhibitor NLG919 and chemotherapeutic doxorubicin via a redox-responsive immunostimulatory polymeric prodrug carrier. Acta Pharmacologica Sinica, 38(6), 823-834.
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7

Polymer Characterization by Advanced Techniques

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The structures of monomer and polymers were characterized by 1H NMR spectrum on a Varian 400 FT-NMR spectrometer (400.0 MHz). FTIR spectra were recorded on a Bio-Rad FTS-185 spectrometer at room temperature. The molecular weight (Mn and Mw) and polydispersity index (Mw/Mn) of polymers were determined by gel permeation chromatography (GPC) with a Waters 2414 refractive index detector. A series of commercial polystyrene standards were used for calibration curves. The average particle size, size distribution and morphology of micelles were measured by dynamic light scattering (DLS, Malvern Zeta Sizer) and transmission electron microscopy (TEM).
Sun J., Chen Y., Xu J., Song X., Wan Z., Du Y., Ma W., Li X., Zhang L, & Li S. (2020). High Loading of Hydrophobic and Hydrophilic Agents via Small Immunostimulatory Carrier for Enhanced Tumor Penetration and Combinational Therapy. Theranostics, 10(3), 1136-1150.
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8

Polymer Micelle Characterization

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1H NMR spectra were examined on a Varian-400 FT-NMR spectrometer at 500 MHz by using D2O or DMSO-d6 as the solvent depending on polymer solubility. The size distribution and zeta potential of all micelles were investigated by dynamic light scattering (DLS) (Zs90; Malvern Instruments, Malvern, UK). The morphology was observed by transmission electronic microscopy (TEM) (Hitachi Ltd., Tokyo, Japan).
Li J., Xu R., Lu X., He J, & Jin S. (2017). A simple reduction-sensitive micelles co-delivery of paclitaxel and dasatinib to overcome tumor multidrug resistance. International Journal of Nanomedicine, 12, 8043-8056.
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9

Polymer Characterization by NMR and GPC

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1H NMR spectrum was examined on a Varian-400 FT-NMR spectrometer at 400.0 MHz with CDCl3 and DMSO-d6 as the solvent. Molecular weight (Mn and Mw) and distribution (Mw/Mn) of the synthesized polymers were measured by gel permeation chromatography (GPC) performed on a Waters 515 HPLC pump and a Waters 717 Plus Autosampler equipped with a Waters 2414 refractive index detector. Tetrahydrofuran (THF) was used as the eluent with a flowing rate of 1.0 mL/min at 35°C. A series of commercial polystyrene standards with narrow molecular weight distribution were applied to calibrate the GPC elution traces.
Sun J., Liu Y., Chen Y., Zhao W., Zhai Q., Rathod S., Huang Y., Tang S., Kwon Y.T., Fernandez C., Venkataramanan R, & Li S. (2017). Doxorubicin Delivered by A Redox-responsive Dasatinib-containing Polymeric Prodrug Carrier for Combination Therapy. Journal of controlled release : official journal of the Controlled Release Society, 258, 43-55.
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10

Polymer Characterization via 1H-NMR

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1H-NMR spectrum of synthesized polymer was examined on a Varian-400 FT-NMR spectrometer at 400.0 MHz with DMSO-d6 as the solvent.
Huang H., Huang Y., Chen Y., Luo Z., Zhang Z., Sun R., Wan Z., Sun J., Lu B., Zhang L., Hu J, & Li S. (2021). A novel immunochemotherapy based on targeting of cyclooxygenase and induction of immunogenic cell death. Biomaterials, 270, 120708.
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