Anti bax mab

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-Bax mAb is a mouse monoclonal antibody that recognizes Bax, a pro-apoptotic protein belonging to the Bcl-2 family. It can be used to detect and study the expression and localization of Bax in various cell and tissue samples.

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Lab products found in correlation

Ripa buffer, by Beyotime (1 mentions) Anti cleaved parp mab, by Cell Signaling Technology (1 mentions) Anti β actin, by ZSGB-BIO (1 mentions) Hrp conjugated secondary antibody, by Southern Biotech (1 mentions) Goat anti mouse mfg e8 antibody, by R&D Systems (1 mentions) Anti β actin mab, by Cell Signaling Technology (1 mentions) Anti bcl2 mab, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-Bax (500 citations) Anti-Bax (D2E11) rabbit mAb (2 citations)

2 protocols using anti bax mab

Apoptosis Signaling Pathway Analysis

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Cells were lysed in RIPA buffer (Beyotime, Shanghai, China) supplemented with protease inhibitors in ice for 30 min followed by centrifugation at 12,000 × g for 15 min at 4°C. Immunoblotting was performed using the anti-cleaved PARP mAb (1:1,000), anti-Bax mAb (1:1,000; Cell Signaling Technology, Danvers, MA, USA), anti-cytochrome c mAb (1:500), anti-caspase-3 mAb (1:1,000; Abcam, Cambridge, MA, USA), anti-HBc mAb (1:500; Milipore, Billerica, MA, USA), anti-β-actin and anti-secondary antibodies were purchased from ZSGB-BIO (Beijing, China).

Gao W.Y., Li D., Cai D.E., Huang X.Y., Zheng B.Y., Huang Y.H., Chen Z.X, & Wang X.Z. (2016). Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore. Oncology Reports, 37(1), 48-56.

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Western Blot Analysis of Apoptotic Proteins

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Proteins from the lung tissues were separated by SDS-PAGE, and the MFG-E8 protein was detected with a goat anti-mouse MFG-E8 antibody (R&D Systems). The lung proteins were incubated with rabbit anti-Bcl-2 mAb, anti-Bcl-XL mAb, anti-Bax mAb and anti-β-actin mAb (Cell Signaling Technology, Danvers, MA, USA) and then visualized with an HRP-conjugated secondary antibody (Southern Biotech, UAB, Birmingham, AL, USA) and chemilumiescence (Thermo Pierce, Rockford, IL, USA) to further detect apoptosis.

Huang W., Wu J., Yang H., Xiong Y., Jiang R., Cui T, & Ye D. (2016). Milk fat globule-EGF factor 8 suppresses the aberrant immune response of systemic lupus erythematosus-derived neutrophils and associated tissue damage. Cell Death and Differentiation, 24(2), 263-275.

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