Mouse anti-beta-Actin (Clone AC-15; Sigma-Aldrich), goat anti-TRPP2 (G-20; Santa Cruz Biotechnology), mouse anti-Polycystin-1 (7E12; Santa Cruz Biotechnology), chicken anti-GFP (ab13970; Abcam), mouse anti-Flag M2 (clone M2; Sigma-Aldrich), rabbit anti-V5 epitope tag (Merck Millipore), and mouse anti-V5-Tag (Clone SV5-Pk1; Bio-Rad) antibodies were obtained commercially. Mouse anti-TRPP2698–799 antibodies have been described previously [12 , 13 (link)]. Western blot detection was performed using an anti-mouse (Dako) horse-radish peroxidase-coupled secondary antibody. Antigens were visualized by immunofluorescence using secondary goat anti-chicken Alexa Fluor 488 (Thermo Fisher Scientific).
Rabbit anti v5 epitope tag
Manufactured by Merck Group
The Rabbit anti-V5 epitope tag is a laboratory reagent used to detect and identify proteins that have been engineered to contain the V5 epitope tag. The V5 tag is a short amino acid sequence that can be fused to recombinant proteins, allowing them to be recognized by this antibody. This antibody can be used in various immunoassays, such as Western blotting and immunoprecipitation, to study the expression and localization of V5-tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti trpp2 g 20, by Santa Cruz Biotechnology (2 mentions)
Mouse anti v5 tag, by Bio-Rad (2 mentions)
Alexa fluor 488 goat anti chicken, by Thermo Fisher Scientific (1 mentions)
Donkey anti rabbit alexa 488, by Thermo Fisher Scientific (1 mentions)
Goat anti chicken alexa 488, by Thermo Fisher Scientific (1 mentions)
Donkey anti mouse alexa 488, by Thermo Fisher Scientific (1 mentions)
Donkey anti mouse cy3, by Jackson ImmunoResearch (1 mentions)
Ab13970, by Abcam (1 mentions)
2 protocols using rabbit anti v5 epitope tag
1
Antibody Detection Techniques for Proteins
2
Immunoblotting and Immunofluorescence Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Rabbit anti-acetyl-α-tubulin (Lys40) (D20G3) (#5335; Cell Signaling), chicken anti-GFP (ab13970; Abcam), mouse anti-Pyruvate dehydrogenase E2/E3bp (ab110333; Abcam), goat anti-TRPP2 (G-20; Santa Cruz Biotechnology), rabbit anti-V5 epitope tag (Merck Millipore), and mouse anti-V5-Tag (Clone SV5-Pk1; Bio-Rad) antibodies were obtained commercially. Monoclonal anti-TRPP2698–799 antibodies and the anti-Amo antiserum have been described previously [12 (link),58 (link)]. Western blot detection was performed using an anti-mouse (Dako) or anti-rabbit (GE Healthcare) horseradish peroxidase–coupled secondary antibody. Antigens were visualized by immunofluorescence using secondary goat anti-chicken Alexa 488 (Invitrogen), donkey anti-mouse Alexa 488 (Invitrogen), donkey anti-rabbit Alexa 488 (Invitrogen), and donkey anti-mouse Cy3 (Jackson Immunoresearch) antibodies.
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