β glycerophosphate

The test implants (Lynx, Equinox, Netherlands) of size 2 mm diameter X 5 mm length were coated with osteogenic nanofibres composed of composite blend of polycaprolactone 5.0% (w/v) (Sigma Aldrich, USA), gelatin type A 0.5% (w/v) (Sigma Aldrich, USA), dexamethasone 0.032% (w/v) (HiMedia, India), β-glycerophosphate 0.5% (w/v) (HiMedia, India), ascorbic acid 0.04% (w/v) (HiMedia, India) and hydroxyapatite 0.04% (w/v) (Budenheim, Germany) in 2,2,2-Trifluoroethanol (Sigma Aldrich, USA). Uncoated titanium screw type implants were used as controls. The fabrication of osteogenic nanofibrous coating on the surface of the implants by electrospinning technique was performed by placing the titanium screw (attached to the rotating shaft of DC motor) in between the syringe tip and collector plate (Fig. 2). The modification of electrospinning apparatus for fabricating nanofibrous coating and its subsequent physico-chemical characterization has been described in detail in our previous study^{24 (link)}.