Lysis buffer (#AS1004, Aspen, South Africa) containing 1% protease inhibitor (#AS1008, Aspen) was used to lyse cells and tissue samples. Proteins were separated via SDS-PAGE, transferred to NC membranes (#IPVH00010, Millipore, USA), blocked with 5% nonfat milk, and stained overnight at 4°C with antibodies specific for ALP (1:1000, Sigma, USA,#ab95462), Osteocalcin (1:500, Sigma, USA, #ab93876), RunX2 (1:500, Sigma, USA, #ab23981), p53 (1:1,000, Sigma, USA, #SAB1302059), and GAPDH (1:10,000, Sigma, USA, #ab37168). Blots were then stained with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (#AS1058, Aspen) and proteins were detected with a chemiluminescence detection system. Each experiment was independently repeated three times.
Ab95462
Manufactured by Merck Group
Sourced in United States
Ab95462 is a multi-channel pipette used for accurate and precise liquid handling. It features adjustable volume settings and ergonomic design to support efficient laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
Ab93876, by Merck Group (4 mentions)
Ab23981, by Merck Group (4 mentions)
Ab37168, by Merck Group (4 mentions)
As1004, by Aspen (4 mentions)
As1008, by Aspen (4 mentions)
Sab1302059, by Merck Group (4 mentions)
As1058, by Aspen (4 mentions)
Ipvh00010, by Merck Group (4 mentions)
Ab34710, by Merck Group (3 mentions)
Sab3500695, by Merck Group (3 mentions)
4 protocols using ab95462
1
Western Blot Analysis of Osteogenic Markers
2
Quantitative Protein Analysis of Osteogenic Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lysis buffer (#AS1004, Aspen, South Africa) containing 1% protease inhibitor (#AS1008, Aspen) was used to lyse cells or callus samples, after which protein was separated via SDS-PAGE and transferred to NC membranes (#IPVH00010, Millipore, USA) that were blocked with 5% nonfat milk and stained overnight at 4 °C overnight with antibodies specific for collagen I (1:500, Sigma, USA,#ab34710), ALP (1:1000, Sigma, USA,#ab95462), Osteocalcin (1:500, Sigma, USA, #ab93876), RunX2 (1:500, Sigma, USA, #ab23981), SIK2 (1:1000, Sigma, USA, #SAB1302059), SIK3 (1:1000, Sigma, USA, #SAB3500695), and GAPDH (1:10,000, Sigma, USA, #ab37168). Blots were then stained with appropriate secondary antibodies conjugated to horseradish peroxidase (HRP) (#AS1058, Aspen), and proteins were detected with a chemiluminescence detection system. Each experiment was repeated three times.
3
Protein Expression Analysis in Cell/Callus Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lysis buffer (#AS1004, Aspen, South Africa) containing 1% protease inhibitor (#AS1008, Aspen) was used to lyse cells or callus samples, after which protein was separated via SDS-PAGE and transferred to NC membranes (#IPVH00010, Millipore, USA) that were blocked with 5% nonfat milk and stained overnight at 4℃ overnight with antibodies speci c for collagen I (1:500, Sigma, USA, #ab34710), ALP (1:1000, Sigma, USA,#ab95462), Osteocalcin (1:500, Sigma, USA, #ab93876), RunX2 (1:500, Sigma, USA, #ab23981), SIK2 (1:1,000, Sigma, USA, #SAB1302059 ), SIK3 (1:1,000, Sigma, USA, #SAB3500695), and GAPDH (1:10,000, Sigma, USA, #ab37168). Blots were then stained with appropriate secondary antibodies conjugated to horseradish peroxidase (HRP) (#AS1058, Aspen), and proteins were detected with a chemiluminescence detection system. Each experiment was repeated three times.
4
Protein Expression Analysis in Cell/Callus Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lysis buffer (#AS1004, Aspen, South Africa) containing 1% protease inhibitor (#AS1008, Aspen) was used to lyse cells or callus samples, after which protein was separated via SDS-PAGE and transferred to NC membranes (#IPVH00010, Millipore, USA) that were blocked with 5% nonfat milk and stained overnight at 4℃ overnight with antibodies speci c for collagen I (1:500, Sigma, USA, #ab34710), ALP (1:1000, Sigma, USA,#ab95462), Osteocalcin (1:500, Sigma, USA, #ab93876), RunX2 (1:500, Sigma, USA, #ab23981), SIK2 (1:1,000, Sigma, USA, #SAB1302059 ), SIK3 (1:1,000, Sigma, USA, #SAB3500695), and GAPDH (1:10,000, Sigma, USA, #ab37168). Blots were then stained with appropriate secondary antibodies conjugated to horseradish peroxidase (HRP) (#AS1058, Aspen), and proteins were detected with a chemiluminescence detection system. Each experiment was repeated three times.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!