Equivalent amounts of whole cell lysate were separated by 10% SDS-PAGE, and then transferred to a 0.45 μm nitrocellulose membrane. Membranes were blocked with 5% skim milk, and incubated overnight at 4 °C with the following antibodies: E-cadherin (BD Biosciences, San Jose, CA), cytokeratin-18 and cellular fibronectin (both from Sigma-Aldrich, Oakville, ON, Canada), vimentin, phospho-ERK1/2 mitogen activated protein kinase (MAPK), total-ERK1/2 MAPK, phospho-p38 MAPK, total-p38 MAPK, SLUG, SNAIL, or phospho-SMAD 2/3 (all from Cell Signaling, Danvers, MA). Membranes were then stripped and re-probed for the housekeeping protein, β-tubulin (Sigma-Aldrich, Oakville, ON, Canada).
Phospho erk1 2 mitogen activated protein kinase
Manufactured by Cell Signaling Technology
Sourced in Canada, United States
Phospho-ERK1/2 mitogen activated protein kinase (MAPK) is a lab equipment product that detects and measures the phosphorylation levels of ERK1/2, which are members of the MAPK family. The product is used to analyze cellular signaling pathways and can be utilized in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Cellular fibronectin, by Merck Group (1 mentions)
Phospho smad2 3, by Cell Signaling Technology (1 mentions)
Total p38 mapk, by Cell Signaling Technology (1 mentions)
Cytokeratin 18, by Merck Group (1 mentions)
β tubulin, by Merck Group (1 mentions)
Snail, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
Phospho p38 mapk, by Cell Signaling Technology (1 mentions)
E cadherin, by BD (1 mentions)
P47phox, by Santa Cruz Biotechnology (1 mentions)
P22phox, by Santa Cruz Biotechnology (1 mentions)
Phospho pkcθ, by Cell Signaling Technology (1 mentions)
2 protocols using phospho erk1 2 mitogen activated protein kinase
1
Western Blot Analysis of Epithelial-Mesenchymal Markers
2
Assessing Oxidative Stress Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dihydroethidine (DHE), lucigenin, rottlerin (Rotln), PD98059, SB203580, myristoylated PKCθ pseudosubstrate (mPKCθ) and PKCβ inhibitor (3-(1-(3-imidazol-1-ylpropyl)-1H-indol-3-yl)-4-anilino-1H-pyrrole-2,5-dione) were purchased from Calbiochem (Billerica, MA, USA). Phospho-PKCθ, phospho-Erk1/2 mitogen-activated protein kinase (MAPK) and α-actin antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA), and p47phox, p22phox, NADPH oxidase 1 (Nox1), Nox2, Nox4 and NoxO1 antibodies and small interfering RNAs (siRNAs) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Cl− channel blocker, 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) was purchased from Calbiochem. All other reagents were purchased from Sigma (St Louis, MO, USA), unless otherwise stated.
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