Cobas enzymatic assay

Baseline information on education, lifestyle and medical history was obtained from self-administered questionnaires. Weight and height and hip and waist circumference were measured during a visit to a study centre. Body mass index, (BMI, weight (kg) divided by height squared (m²)) and waist-hip ratio (WHR, waist circumference/hip circumference) were calculated, adjusted for clothing by subtracting 1.5 kg for weight and 2.0 cm for circumferences for people that were normally dressed without shoes. Physical activity was classified as inactive, moderately inactive, moderately active, and active, according to the Cambridge Physical Activity Index (27 (link)).
The lipids HDL-cholesterol, triglycerides, and lipoprotein (a) were measured in serum using a Cobas enzymatic assay (Roche Diagnostics, Mannheim, Germany) on a Roche Hitachi Modular P analyser. LDL-cholesterol was calculated by Friedewald equation.
Erythrocyte HbA1c was measured using Tosoh (HLC-723G8) ion exchange high-performance liquid chromatography on a Tosoh G8.

Non-fasting blood samples were taken at baseline. Laboratory measures were carried out by the Stichting Huisartsen Laboratorium Groep (Etten-Leur, the Netherlands) on serum (except for participants in the Umea center (Sweden), where only plasma samples were available) or erythrocyte samples that had been previously frozen at either in ultra-low temperature freezers at −80°C or in liquid nitrogen. Serum uric acid, triglycerides, glucose and HDL were measured using a Cobas® enzymatic assay (Roche Diagnostics, Mannheim, Germany) on a Roche Hitachi Modular P analyser. Erythrocyte HbA1c was measured using Tosoh (HLC-723G8) ion exchange high-performance liquid chromatography on a Tosoh G8.