400 mhz instrument

Manufactured by JEOL

Sourced in Japan

The 400 MHz instrument is a high-performance laboratory equipment designed for advanced analytical applications. It provides a core function of nuclear magnetic resonance (NMR) spectroscopy at a frequency of 400 MHz, allowing for the detailed analysis and characterization of various chemical compounds and materials.

Automatically generated - may contain errors

Lab products found in correlation

Model v630 uv vis spectrophotometer, by Jasco (2 mentions) Etc 717, by Jasco (1 mentions) Gemcitabine, by Selleck Chemicals (1 mentions) Q exactive plus, by Thermo Fisher Scientific (1 mentions) Lc 2030c 3d plus, by Shimadzu (1 mentions) Deuterium oxide, by Merck Group (1 mentions) 2400 series 2 elemental chns analyser, by PerkinElmer (1 mentions)

5 protocols using 400 mhz instrument

Characterization of Dendrimer Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proton nuclear magnetic resonance (¹H-NMR) spectra were recorded using a 400 MHz instrument (JEOL Ltd., Tokyo, Japan). Fourier transform-infrared (FT-IR) spectra were recorded by using JASCO FTIR 4600 spectrometer (Jasco Inc., Tokyo, Japan). A powder dendrimer sample was sandwiched between KBr plates for the FT-IR measurement.
Dendrimer-containing solutions were prepared using 100 mM buffers (dendrimer 1 mg/mL, buffer 20 mM) at different pH values. Phosphate (pH 6 and higher) and acetate (pH 3.5–6) buffer solutions were prepared. Phosphoric acid was used to adjust the pH to <3.5. The temperature-dependent transmittance at 500 nm was measured with a 1.0 °C/min heating speed using a Jasco Model V-630 UV/Vis spectrophotometer equipped with an ETC-717 (Jasco Inc.). The dendrimer solutions were prepared using heavy water and the temperature-dependent transmittance curves were also measured.

Kojima C., Fu Y, & Tamaki M. (2022). Control of Stimuli Sensitivity in pH-Switchable LCST/UCST-Type Thermosensitive Dendrimers by Changing the Dendrimer Structure. Polymers, 14(12), 2426.

+ Open protocol

+ Expand

Quantifying Dendrimer-Ligand Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proton nuclear magnetic resonance (¹H-NMR) spectra were recorded using a 400 MHz instrument (JEOL Ltd., Tokyo, Japan) to estimate the bound number of Phe and CHex to the dendrimer. The UV-Vis spectra of the FITC-labeled dendrimers were measured by using Jasco Model V630 UV/Vis spectrophotometer (JASCO Inc., Tokyo, Japan). The bound number of FITC was estimated from the calibration curve and the absorbance at 513 nm in the spectra.

Shiba H., Hirose T., Fu Y., Michigami M., Fujii I., Nakase I., Matsumoto A, & Kojima C. (2023). T Cell-Association of Carboxy-Terminal Dendrimers with Different Bound Numbers of Phenylalanine and Their Application to Drug Delivery. Pharmaceutics, 15(3), 888.

+ Open protocol

+ Expand

Synthesis and Cytotoxicity Analysis of Novel Gemcitabine Derivatives

Check if the same lab product or an alternative is used in the 5 most similar protocols

All the chemicals and reagents
were purchased from Sigma-Aldrich, SRL, and Sd fine and were used
without further purification. The hLDHA enzyme was
purchased from Sigma-Aldrich. Pancreatic cancer cells (02.03, 04.03,
03.27) and liver cancer cells (HepG2) were purchased from ATCC and
NCCS, respectively. Gemcitabine was procured from Selleckchem. Stock
solution of 1 mM was prepared in DMSO and used for in vitro cytotoxicity analysis in pancreatic cancer cells. Thin-layer chromatography
(TLC) was performed on silica gel 60 F254 Merck KGaA Germany, and
spots were visualized by iodine vapor or by irradiation with ultraviolet
light (254 nm). Silica gel of 100–200 mesh was obtained for
column chromatography. Melting points (mp) of all 7a–d,
7j, 7l, and 7m and 8a–d, 8j, 8l, and 8m were calculated on a JSGW apparatus and are
uncorrected. Solvent DMSO was used for NMR purchased from Sigma. NMR
spectra were recorded on a Bruker WH-400 spectrometer or JEOL 400
MHz instrument at a ca. 5–15% (w/v) solution
in DMSO-d₆. Mass spectra were recorded
on a Q EXACTIVE PLUS, Thermo Scientific spectrometer. Elemental analysis
was carried out on a Vario ELIII elementor. HPLC analysis was performed
on Shimadzu LC-2030C 3D plus using column XTIMATE C18 and flow rate
1.0 mL/min.

Sharma D., Singh M., Joshi J., Garg M., Chaudhary V., Blankenberg D., Chandna S., Kumar V, & Rani R. (2023). Design and Synthesis of Thiazole Scaffold-Based Small Molecules as Anticancer Agents Targeting the Human Lactate Dehydrogenase A Enzyme. ACS Omega, 8(20), 17552-17562.

+ Open protocol

+ Expand

Characterizing AuNP Payload Release

Check if the same lab product or an alternative is used in the 5 most similar protocols

NMR tubes were washed thoroughly with acetone and dried overnight at 80°C. Samples were dissolved in deuterium oxide (Sigma, 99.9%). Iodine was added to release the payload from the AuNPs. ¹HNMR was run using a JEOL 400 MHz instrument. Data was plotted using Origin program. Peaks were integrated utilizing Delta software.

Talamantez-Lyburn S., Brown P., Hondrogiannis N., Ratliff J., Wicks S.L., Nana N., Zheng Z., Rosenzweig Z., Hondrogiannis E., Devadas M.S, & Ehrlich E.S. (2019). Gold nanoparticles loaded with Cullin-5 DNA increase sensitivity to 17-AAG in Cullin-5 Deficient Breast Cancer Cells. International journal of pharmaceutics, 564, 281-292.

+ Open protocol

+ Expand

Characterization of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The melting points were measured using open capillary tubes and are uncorrected. ¹H, ¹³C and 2D NMR spectra were recorded on a JEOL 400 MHz instrument. Elemental analyses were carried out on a Perkin Elmer 2400 Series II Elemental CHNS analyser. Mass spectra were performed on JEOL-DX303 HF mass spectrometer.

Arumugam N., Almansour A.I., Kumar R.S., Al-thamili D.M., Periyasami G., Periasamy V.S., Athinarayanan J., Alshatwi A.A., Mahalingam S.M, & Menéndez J.C. (2018). Regio and stereoselective synthesis of anticancer spirooxindolopyrrolidine embedded piperidone heterocyclic hybrids derived from one-pot cascade protocol. Chemistry Central Journal, 12, 95.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!