A. castellanii (ATTC® 30234, derived from ATCC® 30011) and A. comandoni (Pb30/40, group 1, genotype T7 [7 ]) were cultured at room temperature in tissue culture bottles (75 ml, Sarstedt AG & Co., Nümbrecht, Germany). A Peptone Yeast Glucose (PYG) Medium 712 consisting of 20 g proteose peptone (BD, Sparks, USA), 1 g yeast extract (BD, Sparks, USA), 950 ml distilled water, 8 ml 0.05 M CaCl2 (AppliChem GmbH, Darmstadt, Germany), 10 ml 0.4 M MgSO4 × 7H2O (AppliChem GmbH, Darmstadt, Germany), 10 ml 0.25 M Na2HPO4 × 7H2O (Carl Roth GmbH & Co. KG, Karlsruhe, Germany), 10 ml 0.25 M KH2PO4 (Carl Roth GmbH & Co. KG, Karlsruhe, Germany), 34 ml 0.1 M Na citrate × 2H2O (Merck KGaA, Darmstadt, Germany), 10 ml 0.005 M Fe(NH4)2(SO4)2 × 6H2O (AppliChem GmbH, Darmstadt, Germany), and 50 ml 2 M glucose (Sigma-Aldrich Chemie GmbH, Munich, Germany)) was used. The medium was renewed once a week by shaking the bottles, removing the old medium with swimming acanthamoebae, and adding fresh medium to the remaining cells.
Fe nh4 2 so4 2 6h2o
Manufactured by AppliChem
Sourced in Germany
Fe(NH4)2(SO4)2 × 6H2O is a chemical compound that can be used as a laboratory reagent. It is a crystalline solid with a light green color. The compound is soluble in water and is commonly used in various chemical applications.
Automatically generated - may contain errors
Lab products found in correlation
Proteose peptone, by BD (2 mentions)
0.05 m cacl2, by AppliChem (2 mentions)
0.25 m na2hpo4 7h2o, by Carl Roth (2 mentions)
0.25 m kh2po4, by Carl Roth (2 mentions)
Yeast extract, by BD (2 mentions)
Tissue culture bottles, by Sarstedt (1 mentions)
0.4 m mgso4 7h2o, by AppliChem (1 mentions)
2 m glucose, by Merck Group (1 mentions)
2 protocols using fe nh4 2 so4 2 6h2o
1
Cultivation of Acanthamoeba spp. in PYG Medium
2
Culturing Acanthamoeba castellanii Trophozoites
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Example 1
Acanthamoeba were cultured according to Gutekunst et al. (Beilstein J Nanotechnol. 2014, 5, 1393-1398). In brief, trophozoites of Acanthamoeba castellanii (A. castellanii, ATTC 30234) were cultured at room temperature in Peptone Yeast Glucose (PYG) 712 medium (20.0 g proteose peptone (BD, Sparks, USA), 1.00 g yeast extract (BD, Sparks, USA), 950 mL dist. H2O, 10.0 mL 0.40 M MgSO4.7H2O (AppliChem, Darmstadt, Germany), 8.00 mL 0.05 M CaCl2 (AppliChem, Darmstadt, Germany), 34.0 mL 0.10 M sodium citrate.2H2O (Merck, Darmstadt, Germany), 10.0 mL 5.00 mM Fe(NH4)2(SO4)2.6H2O (AppliChem, Darmstadt, Germany), 10.0 mL 0.25 M Na2HPO4.7H2O (Roth, Karlsruhe, Germany), 10.0 mL 0.25 M KH2PO4 (Roth, Karlsruhe, Germany), 50.0 mL 2.00 M glucose (Sigma-Aldrich Chemie GmbH, Steinheim, Germany)). In this axenic culture, the PYG 712 medium was regularly exchanged in the cell culture flasks in order to avoid encystment of A. castellanii trophozoites.
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