Adult (4-month-old) WT and Neil1−/−Neil2−/− male mice were sacrificed with Suprane (Baxter) and the brains removed. Transverse slices (400 μm) were cut from the middle and dorsal portion of each hippocampus with a vibroslicer (Leica VT 1200) in artificial cerebrospinal fluid (ACSF, 4 °C, bubbled with 95% O2–5% CO2) containing (in mM): 124 NaCl, 2 KCl, 1.25 KH2PO4, 2 MgSO4, 1 CaCl2, 26 NaHCO3 and 12 glucose. Slices were placed in an interface chamber exposed to humidified gas at 28–32 °C and perfused with ACSF (pH 7.3) containing 2 mM CaCl2 for at least 1 h prior to the experiments. In some of the experiments, DL-2-amino-5-phosphopentanoic acid (AP5, 50uM; Sigma-Aldrich, Oslo, Norway) was added to the ACSF in order to block NMDA-receptor-mediated synaptic plasticity.
Suprane
Manufactured by Baxter
Sourced in Germany
Suprane is a general anesthetic medication used in surgical procedures. It is a volatile liquid that is vaporized and administered through inhalation. Suprane provides anesthesia and pain relief during medical operations.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using suprane
1
Hippocampal Slicing and Electrophysiology
2
In Vivo Imaging of Subcutaneous Xenografts
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All animal experiments were carried out at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) according to the guidelines of German Regulations for Animal Welfare and have been approved by the Landesdirektion Dresden (24-9165.40-4, 24.9168.21-4/2004-1). Four weeks old female NMRI-Foxn1nu/Foxn1nu mice were purchased from JANVIER LABS (St. Berthevin, France). General anesthesia was induced with 10% (v/v) and maintained with inhalation of 8% (v/v) desflurane (Suprane, Baxter, Germany) in 30/10% (v/v) oxygen/air. Luminescence imaging of the subcutaneous injected cells (exposure times 1 s, 10 s, and 60 s) was performed using a dedicated small animal multimodal imaging system (In-Vivo-Xtreme, Bruker, Germany) 10 min after i.p. injection of 200 µl of D-luciferin (15 mg/ml) (ThermoFisher Scientific). In parallel, an X-ray photograph was taken from the same animals at the same position [39 (link)–41 (link)].
3
Biodistribution of Radiolabeled Compound in Rats and Mice
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Wistar rats with a mean body weight 124 ± 25 g of 55 animals and female NMRI nu/nu tumor mice with a body weight 32 ± 2.5 g of 8 animals were used. Animals were anesthetized with desfluran (Suprane, Baxter, Unterschleiβheim, Germany) initially with 10% in a 30% oxygen air mixture. The radiotracer (333 ± 66 kBq in 0.5 mL) was injected into a lateral tail vein and the animals were recovered. The injected dose was calculated from the activities of the syringes before and after the injection. After 5 and 60 min for rats and 24 h for tumor mice, animals were sacrificed under anesthesia. Blood was obtained by heart puncture and the organs and tissues of interest were removed and weighed and the radioactivity was determined using an automated NaI (Tl) well counter Wallac 1470 Wizard (Perkin Elmer Lifescience). The percentage of injected dose of organ (%ID) was calculated for organs that could be completely extracted and the standardized uptake value (SUV) was calculated according
The activity amounts in the urine were calculated as difference between the injected dose and the recovery from all individual organs, tissues, blood and carcass.
The activity amounts in the urine were calculated as difference between the injected dose and the recovery from all individual organs, tissues, blood and carcass.
4
Bioluminescence Imaging of Tumor-Bearing Mice
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All animal experiments were carried out at the Helmholtz Zentrum Dresden Rossendorf (HZDR) according to the guidelines of German Regulations for Animal Welfare and have been approved by the Landesdirektion Dresden (24-9165.40-4, 24.9168.21-4/2004-1). Four weeks old female NMRI-Foxn1nu/Foxn1nu mice were purchased from JANVIER LABS (St. Berthevin, France). General anesthesia was induced with 10% (v/v) and maintained with inhalation of 8% (v/v) desflurane (Suprane, Baxter, Germany) in 30/10% (v/v) oxygen/air. Luminescence imaging (exposure times 1 s, 10 s, and 60 s) was performed using a dedicated small animal multimodal imaging system (Xtreme, Bruker, Germany) 10 min after i.p. injection of 200 μl of luciferin (15 mg/ml) (Thermofisher, Dreieich, Germany). In parallel an X-RAY photograph was taken from the same animals at the same position.
5
General Anesthesia Induction and Monitoring
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Two intravenous accesses with an 18-gauge peripheral intravenous catheter were established in all patients. General anaesthesia was induced with propofol (Propofol Ampul, Fresenius Kabi, Copenhagen, Denmark), fentanyl (Fentanyl Ampul, Johnson & Johnson, NJ, ABD), and rocuronium (Esmeron Ampul, Merck Sharp & Dohme, NJ, ABD) and maintained using 4%-6% desflurane (Suprane, Baxter, Ill, ABD) + 60% air + 40% O2 mixture and a continuous infusion of remifentanil (Ultiva flakon, GlaxoSmithKline, Brentford, England). Patients were monitored using the BIS index to titrate the desflurane concentration and the level of anaesthesia; we aimed to keep a BIS index between 40 and 60. If the BIS index increased above 60, the concentration of desflurane was increased. Patients were intubated with a double-lumen tube (Covidien, Dublin, OH, ABD), and the position of the tube was confirmed using a fiberoptic bronchoscope (Olympus, Hamburg, Germany). After induction of anaesthesia, the radial artery was catheterised to monitor continuous arterial pressure and analyse blood gas samples, and a central venous catheter was placed in the right internal jugular vein to evaluate central venous pressure.
6
Hippocampal Slice Preparation from Rat Strains
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Experiments were performed on hippocampal slices prepared either from WKY (n=7) or SHR (n=8). The animals were killed with inhalation anaesthetic desflurane (Suprane, Baxter), the brains were removed and transverse slices (400 m) were cut from the middle portion of each hippocampus with a vibroslicer in artificial cerebrospinal fluid (ACSF, 4°C, bubbled with 95% O2 -5% CO2, pH 7.4) containing (in mM): 124 NaCl, 2 KCl, 1.25 KH2PO4, 2 MgSO4, 1 CaCl2, 26 NaHCO3 and 12 glucose. Slices were placed in a humidified interface chamber where the temperature was kept constant at 30°C and they were perfused with ACSF now containing 2 mM CaCl2.
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